G-Biosciences, Buffers, Chemicals
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SDS-PAGE Running Buffer
G-BiosciencesFeatures SDS-PAGE Running Buffer (10X) Compatible with Tris-Glycine gels Separate proteins in the range 6-200 kDa under denaturing conditions 1 X concentration: 25mM Tris Base, 192 mM Glycine, 0.1% SDS Applications Tris-Glycine/SDS Running Buffer [10X] is compatible…
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TBST [10X]; Tris buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Tris Buffered Saline with Tween® 20. The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5. The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5. TBST…
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JAW™ Borate Buffer Packs
G-BiosciencesJAW™ Borate Buffer Packs are dry blend powder pouches used to make borate buffer which is used in protein modification procedures that require amine free and alkaline pH conditions. Features : Each Pack is dissolved in 500 ml deionized water to get 50 mM borate buffer, pH8.5 Come…
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RBC Lysis Buffer
G-BiosciencesOur RBC Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, when used as directed. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood…
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TE Buffer [10X]
G-BiosciencesSynonyms: TE Buffer, Tris-EDTA Buffer, T10E1 Buffer The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. The TE Buffer [10X] is offered in 0.5 and 1L…
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Sodium Bicarbonate
G-BiosciencesFeatures Synonym: Natron CAS#: 144-55-8 Molecular Formula: NaHCO3 Molecular Weight: 84.01
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Modified Dulbecco's PBS Packs (for ELISA and Western Blots) are dry-blend powder pouches used to make physiological Sodium- and Potassium- buffer saline (D-PBS) for ELISA and Western blot diluents and wash buffers. Features : Each pouch when dissolved in DI water and made to final…
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Sodium Phosphate (Dibasic)
G-BiosciencesFeatures Synonym: Disodium hydrogen phosphate CAS#: 7558-79-4 Molecular Formula: Na2HPO4 Molecular Weight: 231-448-7
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STM Buffer
G-BiosciencesFor growing cells and for competent cells preparation Features 270mM Sucrose, 10mM Tris-HCl, ph 7.5, 1mM MgCl2
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Tris
G-BiosciencesTris is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane, with the formula (HOCH2)3CNH2. Tris is extensively used in as a major component in buffer solutions. This 1M solution is has a pH of 9.0. Other 1M Tris solutions are…
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JAW™ Tris-HEPES-SDS Buffer Packs
G-BiosciencesJAW™ Tris-HEPES-SDS Buffer Packs are ready to use dry-blend powder pouches used to make running buffer for SDS-PAGE with Tris-HEPES gels Features : Each pack when dissolved in deionized water and made to final volume of 500 ml makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8 Come in…
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Features Synonym: 1,4-Piperazinediethanesulfonic acid sesquisodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) sesquisodium salt CAS#: 100037-69-2 Molecular Formula: …
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TBE-Urea Sample Buffer
G-BiosciencesLoading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands. For the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies,…
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Potassium Phosphate (Monobasic)
G-BiosciencesFeatures Synonym: Monopotassium phosphate CAS#: 7778-77-0 Molecular Formula: KH2PO4 Molecular Weight: 136.09
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STET Solution
G-BiosciencesBuffer for DNA/RNA extraction and purification. Features 8% w/v sucrose, 5% v/v Triton® X-100, 50mM EDTA, 50mM Tris-HCl, pH 8.0
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IgG Binding, Wash & Elution Buffers
G-BiosciencesG-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.The buffers are ideal for the purification of antibodies from Protein A , Protein G and Protein A/G resins. IgG Binding…
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Tris-EDTA-NaCl Buffer, pH 8.0
G-BiosciencesTris-EDTA-NaCl Buffer solution at pH 8.0. A customer enquired about this buffer to make as custom and we also added as a new product. Features Precision in concentration and pH Guaranteed reproducibility Applications As a buffering mix
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SSC [20X]
G-BiosciencesSaline-Sodium Citrate HybridizationBuffer [20X] controls stringency during the washing steps in Southern blotting, insitu hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features 0.3M trisodium…
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High Molecular Weight Transfer Buffer [5X]
G-BiosciencesBased on Efficient ™ Western Transfer Buffer, the High Molecular Weight Transfer buffer is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70kDa) during Western blotting. Supplied as 1 liter of a 5X concentrated solution.
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PBST [10X]; Phosphate buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4. PBST is commonly used as a wash solution for Western blot…
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RAB Buffer (Reassembly Buffer)
G-BiosciencesA high salt RAB (Reassembly) buffer for the lysis of mammalian cells, including CHO 1,2 , COS 3 , NT2N 4,5 and HEK293 10 ; C. elegans 6,10 and brain tissue 7-9 . Designed for the extraction of soluble proteins, does not extract detergent extractable insoluble proteins. Features …
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TE Buffer [1X]
G-BiosciencesTE Buffer [1X], pH 8.0, Low EDTA (Tris-EDTA; 10mM Tris base, 0.1mM EDTA) are available in different sizes. Other concentrations of TE Buffer are offered, including a 100X concentration and a 10X concentration. 10X and 100X regular TE Buffers are also available Features Low EDTA…
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ACD (Acid Citrate Dextrose) Solutions
G-BiosciencesACD (Acid Citrate Dextrose) Solution A andACD (Acid Citrate Dextrose)Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma. Features Solution A: 38mM citric acid, 107mM sodium citrate, 136mM dextrose Solution B: 23mM citric acid,…
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JAW Dry Powder Buffer Packs
G-BiosciencesPremixed buffer powders, just add water to generate a ready-to-use buffer. JAW™ Carbonate-Bicarbonate Buffer packs make 1L of 0.2M Carbonate-Bicarbonate buffer, pH 9.4. JAW™ Phosphate Buffered Saline (PBS) packs [1X] make 1L of 2.7mM potassium chloride, 137mM sodium chloride…
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Acetate Buffer
G-BiosciencesAcetate Buffer at pH 5.0 and 5.5 are supplied as ready to use solutions that eliminates the calculation, formulation and weighing errors. Features Precision in pH Guaranteed reproducibility
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SDS-PAGE Sample Loading Buffers
G-BiosciencesThe SDS-PAGE Sample Loading Buffers are suitable for loading protein samples on to the SDS-polyacrylamide gels. The buffers are provided in 2X and 6X concentrations containing Tris-HCl, glycerol, SDS and bromophenol blue (BPB) in recommended concentrations and is stable at room temperature. …
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Phosphate Buffers
G-BiosciencesPhosphate buffers are buffer solutions commonly used in biological research. They are water based salt solution containing sodium phosphate monobasic and dibasic at a fixed pH. Competitive remarks and statements Feature statements Application statements Features Useful pH…
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TE Buffer [100X]
G-BiosciencesTE Buffer (Tris-EDTA Buffer) is a common molecular biology buffer that is used to protect DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. Suitable for 10 liters of 1X TE Buffer Features Synonyms: TE Buffer,…
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PBS [10X] (Phosphate Buffered Saline)
G-BiosciencesPBS (Phosphate Buffered Saline) Ready-to-use solutions, available in 1X and 10X concentrated solutions. Features 1X: 8mM NaH2PO4, 150mM NaCl, 3mM KCl, 2mM KH2PO4 (pH 7.4) 10X: 80mM NaH2PO4 1.5M NaCl,…
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TBE [10X] (0.9M Tris-borate, 0.01M EDTA (pH8.3))
G-BiosciencesFeatures 0.9M Tris-borate, 0.01M EDTA (pH8.3)
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Efficient™ Western Transfer Buffer
G-BiosciencesG-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …
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Tris HCl
G-BiosciencesFeatures Synonym: Tris(hydroxymethyl)aminomethanehydrochloride CAS#: 1185-53-1 Molecular Formula: C4H11NO3 · HCl Molecular Weight: 157.6