bioWORLD, 1 L, Chemicals
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DMSO
bioWORLDACS Grade Freezing point: <18.30°C Residue after evaporation: <0.01% Water: <0.1% A275 (undiluted): <0.20 Titratable acid: <0.001meq/g Substances darkened by potassium hydroxide Caution: Readily absorbed through skin Appearance: Liquid …
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Bradford Protein Assay Kit, 1 L
bioWORLDBradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…
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bioPLUS™ Tris Buffer
bioWORLDThe bioWORLD bioPLUS™ tris buffer is suitable for use in biological and biochemical experiments. It is mainly used in gel electrophoresis. Application: For Research & Laboratory Use Only
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TBS + 1% Tween (10X)
bioWORLDBlot blocking buffer used to block non-specific binding site for antibodies on the membrane. 1% Tween-20 in 10X TBS
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Ethylene glycol
bioWORLDIt is a toxic organic compound used as an automotive antifreeze and a precursor to polymers. It is used as plasticizers in adhesives, lacquers and enamels. It also used as coolant and heat transfer agent. High Purity Grade Acidity: <=0.001% Iron: <=0.0005% Moisture (KF):…
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TBE urea Buffers
bioWORLDThomas No. C995V37-V38 TBE Urea Running Buffer, 10X Running buffer for protein analysis. Technical Information: 10X concentration Tris base (0.89M) Boric Acid (0.89M) EDTA(20mM) Thomas No. C995V39-V40 TBE Urea Sample Buffer (2X) Sample buffer for nucleic…
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Terrific Broth (TB)
bioWORLDHighly enriched medium developed to improve yield in plasmid-bearing E. coli Does not contain Glycerol pH: 7.2 Dehydrated Appearance: light beige, homogeneous powder Prepared Appearance: light to medium amber broth Soluble in water Contains: Bacto…
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All bioWORLD running buffers are prepared with high quality reagents.
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BCIP
bioWORLDAlso known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue,…
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TBE Buffers
bioWORLDThomas No. C995V29-V30 Tris-Borate-EDTA (TBE) Buffer, 10X Liquid Concentrate A buffer widely used for nucleic acid electrophoresis Technical Information: 1X buffer contains 0.089M Tris, 0.089M Borate and 0.002M EDTA pH (1X) @ 25ºC : 8.2-8.4 1L of concentrate makes 10L of 1X…
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MOPS-SDS running Buffer (20X)
bioWORLDMOPS-SDS Running Buffer for Protein Gel electrophoresis SDS running buffer with MOPS and Tris (pH 7.7) 20X concentration All bioWORLD running buffers are prepared with high quality reagents
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Potassium Phosphate Buffer
bioWORLDPotassium phosphate buffer has a concentration of 0.1M and a pH of 7.2. It contains 28.3% of KH2PO4 (monobasic) and 71.7% of K2HPO4 (dibasic). Phosphates have a high buffering capacity and are soluble in water. They inhibit many enzymatic reactions and procedures of molecular cloning. Phosphates…
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Tween 80
bioWORLDA non-ionic nondenaturing detergent used in buffers to solubilize membrane proteins. Commonly used in gels for PAGE isoelectric focusing. Reagent Grade Heavy metals (such as Pb): Also known as Sorbitan Monooleate
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PBS 10X with 0.5% Tween 20, pH 7.5
bioWORLDPBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter…
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bioPLUS™ Phosphate Buffer
bioWORLDPhosphate buffer contains a mixture of monobasic dihydrogen sodium phosphate and a dibasic monohydrogen sodium phosphate. Phosphates have a high buffering capacity and are soluble in water. They inhibit many enzymatic reactions and procedures of molecular cloning. Phosphates also precipitate in…
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Acrylamide and bis-acrylamide are major ingredients in polyacrylamide gels, a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Fragments of DNA produced by various molecular biology techniques (eg. restriction endonuclease digestion, dideoxy sequencing, or the…
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Ham's F-12 (Kaighn's modification)
bioWORLDHam's Nutrient Mixtures, F-10 and F-12, were both originally developed to support the growth of a variety of cells, particularly the Chinese Hamster Ovary (CHO) cells, with or without serum supplementation. Ham's F-10 has since been used in the culture of a wide variety of cell of human and animal…
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DMF (N',N-Dimethylformamide)
bioWORLDReagent grade A strongly polar organic solvent Refractive index: 1.423- 1.431 Hazardous Materials Description: N,N-Dimethylformamide Hazard Class or Division: 3 Identification Numbers: UN2265 Packaging Group: III *Applies additional hazardous materials handling…
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Tween 20
bioWORLDSynonyms: Polysorbate 20; Sorbitan Monolaurate Non-ionic nondenaturing detergent used in buffers to solubilize membrane proteins Commonly used in gels for PAGE isoelectric focusing Reagent Grade Heavy metals (such as Pb):
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MES Buffered Saline, pH 6.1
bioWORLDCommon buffer for biological sciences. 0.1M MES (2-[morpholino]ethanesulfonic acid) and 0.9% NaCl Appearance: Liquid Melting Point: >300°C (lit.)(dec.) PH Value: 6.1 RTECS Number: QE3479500
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Coomassie blue G-250 (2x)
bioWORLDA commonly used analytical biochemistry dye and P2X7 antagonist Stain for location and detection of proteins. Protocol: After SDS-PAGE, transfer the gels in fixing solution (30% methanol and 7% acetic acid) for 30 min. under constant agitation. Replace the fixing solution, submerge the…
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TG (Tris-Glycine) Buffers
bioWORLDTris Glycine (TG) Buffer The TG Buffers are blended using pure reagents to provide the highest quality buffers for protein research. Ideal for protein separation, this buffer is formulated using Pro-Pure Tris powder to ensure exceptional purity and the absence of nuclease and protease…
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bioPLUS™ Water, DEPC-Treated
bioWORLDIt contains one oxygen and two hydrogen atoms connected by covalent bonds. It is commonly used in chemical reactions as a solvent or reactant and less commonly as a solute or catalyst. Application: For Research & Laboratory Use Only
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bioPLUS™ Sodium Phosphate 0.1M
bioWORLDThe bioWORLD bioPlus™ 0.1 M sodium phosphate is composed of sodium phosphate monobasic, sodium phosphate dibasic heptahydrate and water. It is mainly used as a saline laxative. It is also used in manufacturing detergents, soaps and fertilizers.
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IBS
bioWORLDIBS/Casein (Thomas Nos. C994C53 and C994C54) All blocks and diluents are supplied in a 10X concentration of physiological saline buffer. Sold as 2 x 100mL or 500mL. Technical Information: pH range: 7.0 (+/- 0.1) based upon an Imidazole physiologic buffer …
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STE (saline-tris-EDTA) Buffer 10X
bioWORLDSTE Reagent Common reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols. DNAse and RNAse free, 10X solution Technical Information: 1X concentration solution contains NaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)
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Acetate Buffer, 1M
bioWORLDCommon buffer for biological sciences. Ultra Pure Grade 1M Concentration Appearance: Liquid Melting Point: 323-329 °C PH Value: 4.0 RTECS Number: AJ4375000
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Gelatine-Blocking Buffer
bioWORLDBlot blocking buffer used to block non-specific binding site for antibodies on the membrane. 1% Gelatin in PBS
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bioPLUS™ Triton X-114
bioWORLDA non-ionic nondenaturing detergent used to solubilize membrane proteins Commonly used detergent in isoelectric focusing. Application: For Research & Laboratory Use Only, For in Vitro Lab Use Only. Not For Human Use or Consumption
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TAE Buffer, 50X Liquid Concentrate
bioWORLDTris-Acetate-EDTA (TAE) buffer, 50X A common buffer for electrophoresis A 50X solution of tris, acetate, and EDTA in distilled water
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Phosphate Buffered Saline (PBS)
bioWORLD10X PBS solution/li> Sterile Ultra Pure Grade pH (25°C): 7.4 +/- 0.1 DNase Activity: None
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Glycerol
bioWORLDGlycerol (or glycerine, glycerin) is a polyol compound. It is colorless, odorless,viscous liquid that is widely used in biological applications. Synonym: glycerin Common reagent used in tissue culture media for bacteria and enzyme storage. Useful for mammalian cell transfection and…