A commonly used analytical biochemistry dye and P2X7 antagonist
Stain for location and detection of proteins.
Protocol: After SDS-PAGE, transfer the gels in fixing solution (30% methanol and 7% acetic acid) for 30 min. under constant agitation. Replace the fixing solution, submerge the gel in stain and shake the gel gently gor 2-4 hrs. Pour off the stain, rinse the gel briefly with fixing solution and cover the gel with fresh fixing solution for 30 min. Repeat and continue destaining until distinct blue bands against a clear background are obtained. Store the gel in 7% acetic acid solution. Photograph or dry the gel according to your standard protocol.
Synonym: Acid blue 90, Coomassie Brilliant Blue G
Appearance: Liquid
HS Code: 32041200
Melting Point: >100°C