Potassium phosphate buffer has a concentration of 0.1M and a pH of 7.2. It contains 28.3% of KH2PO4 (monobasic) and 71.7% of K2HPO4 (dibasic). Phosphates have a high buffering capacity and are soluble in water. They inhibit many enzymatic reactions and procedures of molecular cloning. Phosphates also precipitate in ethanol making it impossible to precipitate DNA and RNA from buffers that contain large amounts of phosphate ions. Phosphate Buffer is frequently used as a buffering agent in biology and biochemistry. It is prepared by mixing Potassium Phosphate Monobasic and Sodium Phosphate Dibasic Heptahydrate in a fixed ratio to obtain the desired pH and strength.
Application: For Laboratory Use Only. Not Fit For Agricultural, Household, Clinical Use or Human Consumption