Rockland Immunochemicals Inc, Buffers, Chemicals
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Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml), 1Each,…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye™800,…
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10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Dilute only prior to immediate use. 10X Phosphate Buffered Saline (PBS) consists of 0.2 M Potassium Phosphate, 1.5 M Sodium…
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20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC…
Rockland Immunochemicals Inc20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0) DEPC Treated is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 20X SSC pH 7.0 (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH…
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10X HDAC Assay Buffer, 20mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl and 1.0 mM MgCl2 at pH 8.0.
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Fluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) Staining…
Rockland Immunochemicals IncFluorescent Activated Cell Sorting (FACS) and Immunofluorescence (IF) staining buffer is adequate for cell staining, washing and dilution in flow cytometry and immunofluorescence experiments.
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1X RIPA Lysis Buffer, 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncRIPA Lysis Buffer is ready-to-use as a working 1X solution and requires no further dilution. RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…
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1.0 M Tris HCl pH 7.6, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncTris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…
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TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.
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Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml), 1Each,…
Rockland Immunochemicals IncFluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…
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1X RIPA Lysis Buffer 5-PACK (5 x 50 ml), 1Each, Liquid (sterile filtered)
Rockland Immunochemicals IncThis product is ready-to-use as a working 1X solution and requires no further dilution. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein…
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X-GAL BETA-GALACTOSIDASE SUBSTRATE, 100mg, Solid
Rockland Immunochemicals IncX-Gal (5-Bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) produces a dimethylformamide (DMF)-soluble, stable blue precipitate at the reaction site. X-Gal is the classical substrate used with ß-galactosidase in current methods of molecular biology, microbiology and immunology. X-Gal is used to…
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10X RIPA Lysis Buffer
Rockland Immunochemicals IncThis product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and…
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Revitablot™ Western Blot Stripping Buffer
Rockland Immunochemicals IncRevitablot™ Western Blot Stripping Buffer is ready-to-use as a working 1X solution and requires no further dilution.
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1.0 M Tris HCl pH 7.2, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncSuitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 1.0 M…
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10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% …
Rockland Immunochemicals IncPhosphate Buffered Saline 10X pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X PBS pH 7.2 with…
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DAB MEMBRANE PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncDAB Membrane Peroxidase Substrate (50x) is designed to be diluted with part number DAB-B50
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BlockOut® (2X), 50mL, Liquid (sterile filtered)
Rockland Immunochemicals IncBlockOut® is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This product allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and…
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Tris-Glycine
Rockland Immunochemicals IncTris-Glycine running buffer is suitable for laboratory involved in protein biochemistry. This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required. 10X…
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Molecular Biology Grade UltraPure Water
Rockland Immunochemicals IncMolecular Biology Grade Ultrapure water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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20% (w/v) Sodium Dodecyl Sulfate (SDS)
Rockland Immunochemicals IncThis product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 20% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly…
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FETAL BOVINE SERUM Origin: United States triple 0.1 micron filtered
Rockland Immunochemicals IncFetal Bovine Sera sourced from the United States is collected exclusively from USDA licensed abattoirs from within the United States. Raw material is processed through 0.1 µm (100nm) filters in compliance with regulatory guidelines. Animals are inspected pre-mortem and post-mortem by USDA…
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10X Tris-Phosphate-EDTA (TPE) (0.9 M Tris-Phosphate 0.01 M EDTA pH 8.0), 1L,…
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X Tris-Phosphate EDTA (TPE) consists of 0.9 M Tris-Phosphate, 0.02 M EDTA at a pH of 8.0. Meticulously prepared using ultra…
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NORMAL SHEEP SERUM (NSS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Sheep Serum (NMS) is a useful reagent for blocking Western Blots and ELISA wells.
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1X TBS Buffer with 1% Casei
Rockland Immunochemicals IncThis product is a ready-to-use 1X solution and no further dilution is required.
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1X PBS Buffer with 1% Casein
Rockland Immunochemicals IncThis product is a ready-to-use 1X solution and no further dilution is required.
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Blocking Buffer for Immunohistochemistry, 100mL, Liquid (sterile filtered)
Rockland Immunochemicals IncThis immunohistochemistry protein blocker is specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HRP) with diaminobenzidine (DAB). This product specifically inhibits non-specific staining of IHC detection…
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10X TBS Fish Gel Concentrate (Azide and Mercury free)
Rockland Immunochemicals IncThis product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5. A…
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TMB ELISA PEROXIDASE SUBSTRATE
Rockland Immunochemicals IncTMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.
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UltraPure Sterile Water, 1L, Liquid (sterile filtered)
Rockland Immunochemicals IncUltra Sterile Water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.
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NORMAL RAT SERUM (NRS), 10mL, Lyophilized
Rockland Immunochemicals IncNormal Rat Serum (NRS) is a useful reagent for blocking Western Blots and ELISA wells.
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1X SDS-PAGE Destain Solution (Ready-to Use), 1L, Liquid
Rockland Immunochemicals Inc1X SDS-PAGE Destain Solution is ready-to-use as a working solution and requires no further dilution.
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1.0 M HEPES pH 7.2
Rockland Immunochemicals Inc1.0 M HEPES pH 7.2 is a concentrated stock solution and should be diluted appropriately with molecular biology grade water or equivalent to its final working concentration. This buffer consists of 1.0 M HEPES at a pH of 7.2 and was prepared using ultra pure reagents dissolved in DEPC treated…