Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye™800,…

Phosphate Buffered Saline 10X pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 10X PBS pH 7.2 with…

The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

Borate Saline Buffered (BSB) is commonly used in coating procedures for ELISA. BBS can also be used as a component in blocking solutions for Western blotting. The buffer can be used as a diluent in biological samples and for various applications within biology and biochemistry to maintain the pH.

NPP (1X) ELISA ALKALINE PHOSPHATASE SUBSTRATE, 100mL, Liquid - clear to pale yellow, Expiration date is one (1) year from date of opening.

This product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline, and proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A…

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS).  Meticulously prepared using ultra pure reagents dissolved in highly…

BlockOut® is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This product allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and…

ABTS ELISA PEROXIDASE SUBSTRATE is a ready to use substrate - No dilution required. Read absorbance of blue green color at 405 nm. The color formation as a function of time can be recorded at intervals or the reaction may be stopped by addition of acid after 30 mins. A 0.625 Mol L-1 Oxalic Acid…

BCIP/NBT Alkaline Phosphatase Membrane Substrate is a useful detection reagent for immunoblotting. The substrate is ready to use. To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color…

TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB ELISA Peroxidase Substrate comes ready to use.

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of  50 mM Tris HCl, 10 mM Magnesium Sulfate at pH 7.5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionized water.

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5.  Meticulously prepared using ultra pure reagents dissolved in…

5.0 Ammonium Acetate is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 5.0 M Ammonium Acetate.  Meticulously prepared using ultra pure reagents dissolved in highly…

TMB Membrane Peroxidase Substrate comes ready to use. No dilutions are required. TMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation…

Suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of  1.0 M…

This product is a 10X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X TBS Fish Gel Concentrate consists of 1.0 M Tris hydrochloride, 1.5 M sodium chloride and Fish Gelatin at pH 7.5.  A…

BBS is isotonic and has a strong bactericidal effect. It can be used to dilute solutions for coating procedures. Additives such as Tween 20 and Blotto can be used to add functionality as a washing buffer or blocking buffer.

Must be diluted

Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk.   Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available.  BLOTTO A is a…

This product is 10X concentrated stock solution. Dilute to 1X prior to use. 1X RIPA Lysis Buffer is intended for the extraction of cellular proteins for the efficient lysis of cells and solubilization of protein, while minimizing protein degradation and maintaining protein immunoreactivity and…

This product is a ready-to-use 1X solution and no further dilution is required.

Utilized as the diluent buffer in ELISA experiments

TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.

Tris HCl solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This buffer consists of 1.0 M Tris HCl at pH 8.5. Meticulously prepared using ultra pure reagents dissolved in highly polished pharmaceutical grade (WFI) water. This product…

1.0 M HEPES pH 7.2 is a concentrated stock solution and should be diluted appropriately with molecular biology grade water or equivalent to its final working concentration. This buffer consists of 1.0 M HEPES at a pH of 7.2 and was prepared using ultra pure reagents dissolved in DEPC treated…

Ultra Sterile Water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable.

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 20% (w/v) Sodium Dodecyl Sulfate (SDS).  Meticulously prepared using ultra pure reagents dissolved in highly…

This product is a 10X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl and 1.0 mM MgCl2 at pH 8.0.

Fluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in western blotting and other applications.…

TE buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 8.0.  Meticulously prepared using ultra pure reagents dissolved in DEPC…

Dilute your primary or secondary alkaline phosphatase conjugated antibody or other protein or compound with ALP Conjugate Stabilizer to the desired 1X working concentration determined for your conjugate. ALP Conjugate Stabilizer may be used to dilute conjugates when determining titer. In one step…