10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.2 M Potassium…

TBST is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. May be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.

Borate Saline Buffered (BSB) is commonly used in coating procedures for ELISA. BBS can also be used as a component in blocking solutions for Western blotting. The buffer can be used as a diluent in biological samples and for various applications within biology and biochemistry to maintain the pH.

This product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline, and proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A…

This product is a ''ready-to-use'' 1X diluent for Horseradish Peroxidase (HRP) conjugated primary or secondary. This buffer contains buffered saline along with proprietary reagents to stabilize and preserve Horseradish Peroxidase (HRP) conjugated primary or secondary antibodies in solution. A…

BBS is isotonic and has a strong bactericidal effect. It can be used to dilute solutions for coating procedures. Additives such as Tween 20 and Blotto can be used to add functionality as a washing buffer or blocking buffer.

Tween-20 Ultra Pure, Liquid

UniGlow™ is ready-to-use and no mixing is required. UniGlow™- One Component Chemiluminescent Substrate is for use in microwell or membrane applications. Protect from light - UniGlow™ Substrate is a highly sensitive detection reagent. Equilibrate to room temperature before use and aliquot into a…

Ethylenediaminetetraacetic acid is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.5 M Trisodium EDTA at pH 8.0.  Meticulously prepared using ultra pure reagents dissolved…

This product is a 10X concentrated stock solution and should be diluted appropriately with distilled, deionized water (or equivalent) to its final working concentration. No pH adjustment is required.

This buffer is a 10X concentration. Optimum performance is achieved using this product diluted 1:10 in deionized water. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6.

This component is ready-to-use and requires no additional preparation.

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromophenol blue.  The pH of…

This product is a 20X concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 3.0 M Sodium Chloride, 0.2 M Sodium Hydrogen Phosphate, 0.02 M EDTA at a pH of 7.4.  Meticulously prepared…

This product is a 10X concentrated stock solution.  Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. 10X PBS Fish Gel Concentrate consists of 0.2M potassium phosphate, 1.5M sodium chloride and Fish Gelatin at pH 7.2.  A…

Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B).  Mix well.  Protect from light.  Larger or smaller volumes of the substrate can be prepared by…

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1.0 mM EDTA at a pH of 7.8.  Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionized water.

Tris solution is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer…

2X SDS-PAGE Sample Loading Buffer is suitable for laboratory assays involved in protein biochemistry. 2X SDS-PAGE Sample Buffer is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. 2X SDS-PAGE Sample…

Normal Mouse Serum (NMS) is a useful reagent for blocking Western Blots and ELISA wells.

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 100 mM Tris HCl and 10 mM EDTA at a pH of 7.5.  Meticulously prepared using ultra pure reagents dissolved in…

This product is a 1.0M concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer contains untitrated 1.0 M Magnesium Sulfate that was meticulously prepared using ultra pure reagents dissolved in…

10% SDS is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS).  Meticulously prepared using ultra pure reagents dissolved in highly polished…

Horse serum is suitable for blocking, western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

TMB Membrane Peroxidase Substrate will produce an insoluble blue-green end product. Positive signal deposits blue-green color on membrane or surface of sample material. TMB Membrane Peroxidase Substrate incubation time will vary depending on the assay conditions. No dilutions are required, TMB…

This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 10% (w/v) Sodium Dodecyl Sulfate (SDS).  Meticulously prepared using ultra pure reagents dissolved in highly…

Chill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.

This following method is suggested as a guideline for the use for Western Blot with nitrocellulose or PVDF membranes. The TMB Substrate in 100 mL bottle TMBM-101 and the TMB Precipitating Agent in 10 mL bottle TMBM-102 are used together. Mix 9 ml of the TMB Substrate and 1.0 ml of the TMB…

This product is a ''ready-to-use'' 1X solution for stabilizing ELISA plates for storage prior to ELISA. After coating with antigen or antibody, wash the contents of the ELISA microwells and add a sufficient volume of ELISA Microwell Blocking Buffer with Stabilizer to each well of the microplate. …

Tris-Acetate-EDTA (TAE) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Tris-Acetate-EDTA (TAE) consists of 2.0 M Tris-Acetate, 0.05 M EDTA at a pH of 8.3.  Meticulously prepared using ultra pure…

Optimum performance is achieved using this product undiluted.  However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance. This buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary…

ProXtract B™ Buffer is suitable for protein extraction and easily scalable. Specific conditions should be optimized by the end user. See protocol.