G-Biosciences Buffer Solutions
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IgG Binding, Wash & Elution Buffers
G-BiosciencesG-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins.The buffers are ideal for the purification of antibodies from Protein A , Protein G and Protein A/G resins. IgG Binding…
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Sodium Acetate
G-BiosciencesFeatures Synonym: Acetic acid sodium salt CAS#: 127-09-3 Molecular Formula: C2H3O2Na Molecular Weight: 82.03
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Molecular Biology Universal Kit
G-BiosciencesA selection of 12 x 25ml molecular biology relevant buffers. These buffers are routinely used in molecular biology and are all DNase and RNase free. The buffers supplied are listed below. Features Molecular Biology Universal Kit Ammonium acetate [5M] Calcium chloride [1M] …
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FOCUS™ Extraction Buffers
G-BiosciencesChaotropic extraction buffers that preserve the native charge of proteins making them s uitable for sample extraction for 2D gels One of the most important considerations before running 2D gel electrophoresis is the choice of protein solubilization buffers. A suitable buffer must…
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SSC [20X]
G-BiosciencesSaline-Sodium Citrate HybridizationBuffer [20X] controls stringency during the washing steps in Southern blotting, insitu hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features 0.3M trisodium…
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Tris-Acetate/SDS Running Buffer [20X]
G-BiosciencesTris-Acetate/SDS running buffer is used to resolve high molecular weight proteins (36-400 kDa) under denaturing conditions on Tris/Acetate gels Features Tris-Acetate/SDS running buffer is compatible with Tris/Acetate gels Separate high molecular weight proteins (36-400 kDa) under…
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RAB Buffer (Reassembly Buffer)
G-BiosciencesA high salt RAB (Reassembly) buffer for the lysis of mammalian cells, including CHO 1,2 , COS 3 , NT2N 4,5 and HEK293 10 ; C. elegans 6,10 and brain tissue 7-9 . Designed for the extraction of soluble proteins, does not extract detergent extractable insoluble proteins. Features …
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Modified Dulbecco's PBS Packs (for ELISA and Western Blots) are dry-blend powder pouches used to make physiological Sodium- and Potassium- buffer saline (D-PBS) for ELISA and Western blot diluents and wash buffers. Features : Each pouch when dissolved in DI water and made to final…
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TBS [10X] (Tris buffered saline)
G-BiosciencesTBS, Tris buffered saline [10X] 100mM Tris HCl, 1.5M NaCl, pH 7.5
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TBST [10X]; Tris buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Tris Buffered Saline with Tween® 20. The 10X Concentration is 100mM Tris.HCl, 150mM NaCl, 0.5% Tween® 20 at pH7.5. The 1X Concentration is 10mM Tris.HCl, 15mM NaCl, 0.05% Tween® 20 at pH7.5. TBST…
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SSC [20X] (Sodium Chloride-Sodium Citrate)
G-BiosciencesSaline-Sodium Citrate Hybridization Buffer [20X] controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting. 20X SSC may be used to prevent the drying of agarose gels during a vacuum transfer. Features …
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Zymogram Sample Buffer
G-BiosciencesZymography is an electrophoretic technique, based on SDS-PAGE, that includes an enzyme substrate copolymerized with the polyacrylamide gel. Samples are prepared in Zymogram Sample Buffer without boiling to preserve the structure and activity of the enzyme. Following electrophoresis, the SDS is…
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Features Synonym: 1,4-Piperazinediethanesulfonic acid sesquisodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) sesquisodium salt CAS#: 100037-69-2 Molecular Formula: …
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RBC Lysis Buffer
G-BiosciencesOur RBC Lysis Buffer is designed for the optimal lysis of erythrocytes, while having minimal effect of lymphocytes, when used as directed. The use of RBC Lysis Buffer allows for the preferential lysis of red blood cells from whole blood and as these are the majority of cells in whole blood…
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Streptavidin Elution Buffer
G-BiosciencesFeatures 8M Guanidine.HCl, pH 1.5 Streptavidin resin also available
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PBST [10X]; Phosphate buffered saline with Tween-20
G-BiosciencesA 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4. PBST is commonly used as a wash solution for Western blot…
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ACD (Acid Citrate Dextrose) Solutions
G-BiosciencesACD (Acid Citrate Dextrose) Solution A andACD (Acid Citrate Dextrose)Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma. Features Solution A: 38mM citric acid, 107mM sodium citrate, 136mM dextrose Solution B: 23mM citric acid,…
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Tris-EDTA-NaCl Buffer, pH 8.0
G-BiosciencesTris-EDTA-NaCl Buffer solution at pH 8.0. A customer enquired about this buffer to make as custom and we also added as a new product. Features Precision in concentration and pH Guaranteed reproducibility Applications As a buffering mix
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IEF Cathode Buffer (pH 3-7) [10X]
G-BiosciencesIsoelectric Focusing Cathode Buffer, pH3-7 Features pH 3-7
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SDS-PAGE Running Buffer
G-BiosciencesFeatures SDS-PAGE Running Buffer (10X) Compatible with Tris-Glycine gels Separate proteins in the range 6-200 kDa under denaturing conditions 1 X concentration: 25mM Tris Base, 192 mM Glycine, 0.1% SDS Applications Tris-Glycine/SDS Running Buffer [10X] is compatible…
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Acetate Buffer
G-BiosciencesAcetate Buffer at pH 5.0 and 5.5 are supplied as ready to use solutions that eliminates the calculation, formulation and weighing errors. Features Precision in pH Guaranteed reproducibility
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STET Solution
G-BiosciencesBuffer for DNA/RNA extraction and purification. Features 8% w/v sucrose, 5% v/v Triton® X-100, 50mM EDTA, 50mM Tris-HCl, pH 8.0
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TE Buffer [10X]
G-BiosciencesSynonyms: TE Buffer, Tris-EDTA Buffer, T10E1 Buffer The TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help protect DNA and RNA. The TE Buffer [10X] is offered in 0.5 and 1L…
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Potassium Phosphate (Monobasic)
G-BiosciencesFeatures Synonym: Monopotassium phosphate CAS#: 7778-77-0 Molecular Formula: KH2PO4 Molecular Weight: 136.09
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Efficient™ Western Transfer Buffer
G-BiosciencesG-Biosciences scientists have developed a high efficiency transfer buffer for Western blotting. The pre-made and ready-to-use [20X] transfer buffer is prepared for optimal conductivity and efficient protein transfer without generating excessive heat or transfer distortion. Efficient ™ …
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TNT Buffer
G-BiosciencesA general molecular biology and hybridization buffer Features 0.1M Tris·HCl (pH 7.5) 0.15M sodium chloride 0.05% Tween® 20
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JAW Dry Powder Buffer Packs
G-BiosciencesPremixed buffer powders, just add water to generate a ready-to-use buffer. JAW™ Carbonate-Bicarbonate Buffer packs make 1L of 0.2M Carbonate-Bicarbonate buffer, pH 9.4. JAW™ Phosphate Buffered Saline (PBS) packs [1X] make 1L of 2.7mM potassium chloride, 137mM sodium chloride…
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TBE [10X] (0.9M Tris-borate, 0.01M EDTA (pH8.3))
G-BiosciencesFeatures 0.9M Tris-borate, 0.01M EDTA (pH8.3)
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STET-E1 Solution
G-BiosciencesFor DNA/RNA Extraction and Purification Features 3M NaCl, 165mM Tris-HCl, pH 8.0, 45mM EDTA, pH 8.0, and 16.4% Tween® 20
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RIPA Lysis & Extraction Buffer
G-BiosciencesA complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification…
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Tris Base
G-BiosciencesFeatures Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol Linear Formula: NH2C(CH2OH)3 Molecular Weight: 121.14 CAS Number: 77-86-1