DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We offer a variety of DMEM modifications…

L-glutamine is a cell culture supplement, and is required in cell culture for cellular growth. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, gluthione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production.…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate Without HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

EMEM (Eagle’s Minimal Essential Medium) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without L-Glutamine Applications EMEM has a higher amino acid concentration and 2 to 5 times greater…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial…

Dr. Williams and his coworkers’ studies on hepatocytes in culture led to the development of Williams’ Medium E. Without L-glutamine Applications This rich medium was used to culture the hepatocytes derived from an enrichment procedure also developed by Dr. Williams and his colleagues in…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations Without Phenol Red With Glucose and L-glutamine Applications EMEM (Eagle’s Minimum Essential Medium) has…

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction…

Molecular Biology Water is prepared by reverse osmosis, passed through fine carbon, deionized through two resin beds, and serially filtered through 0.1 µm positively charged membranes, to yield a product with an endotoxin level of less than 0.03 EU/mL. DNase, RNase and protease tested Endotoxin…

RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L Sodium Bicarbonate Without HEPES buffer Applications E199 has broad species applicability, particularly for cultivation of…

Hanks' Balanced Salt Solution (HBSS) provides a buffering system to maintain the physiological pH range and osmotic balance of the culture media. HBSS provides cells with a source of water and essential inorganic ions, and carbohydrate as an energy source. The HBSS without Ca and Mg…

LB Agar 1.5% Lennox provides all nutritional requirements of these organisms. LB Agar 1.5% Lennox contains half the sodium chloride level of the Miller formulation of LB Agar. Just autoclave to melt and pour. Applications LB Agar 1.5% Lennox is nutritionally rich. It was developed by…

SOC medium is a ready-to-use solution, useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. Without magnesium chloride, needs to be added before use. Autoclave sterilized Applications SOC is a microbial growth medium used for…

Applications Standard, unstabilized solutions of L-glutamine spontaneously break down over time to release cytotoxic amines. Glutamine XL is a stabilized solution that prevents degradation and cytotoxicity, with the benefit of a long shelf life. The incorporated L-alanine protects the unstable…

Potassium Chloride 1 M is used to establish a constant ionic strength in a sample solution. KCl 74.56 g/L Suitable for PCR, molecular biology, protein chemistry and biochemical applications Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications Potassium…

Fischer’s medium supports clonal growth of cells – particularly lymphoblasts – from primary explants or cells already in culture. With L-glutamine and Phenol Red Applications Fischer’s medium also supports mammalian cell culture and murine hematopoietic cells. Recommendation …

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With: High Glucose Without: HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells. We…

DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With High Glucose Without L-Glutamine Without Phenol red Without HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,…

Super Broth is used for the mass cultivation of Escherichia coli for high yield protein and plasmid DNA production. Has 220% more peptone and 300% more yeast extract than LB Autoclave sterilized Applications Super Broth is the best commonly used E. Coli growth medium for plasmid…

Sodium pyruvate is commonly utilized as an additive in culture media and broth as a carbon source, similar to glucose, as pyruvate is the end product of the glycolysis pathway. Since cells make sodium pyruvate as an intermediate metabolite in the glycolysis pathway, it is not a required supplement…

Designed for cell culture without CO2. Without Sodium Bicarbonate With Phenol Red Without L-glutamine Applications L-15 has been used for the culture of HEp-2 monkey kidney and primary explants of embryonic and adult human tissue, and a variety of other cells. Many viruses have also been…

Sodium Chloride, 5M is suitable for molecular biology, protein chemistry and biochemical applications. 5 M concentration Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications Sodium Chloride, 5 M is free of detectable endonucleases, ribonucleases and nicking…

RPMI was developed to support lymphoblastoid cells in culture, RPMI-1640 has now been successfully used for the culture of a wide range of anchorage independent cells. Although intended for use with serum supplementation, RPMI-1640 has been shown to support the serum-free growth of a number of…

Applications The FBS in the Cryopreservation Medium improves the post-freezing recovery and survival after cryopreservation, while DMSO preserves the cells during freeze. Cryopreservation Medium with Fetal Bovine Serum (FBS) and Calf Serum is a complete cryopreservation medium with 10% DMSO.

Cell Freezing Medium A protects cells from damage after freezing and thawing. Applications Cell Freezing Medium A is frequently used in cell biology research for the cryopreservation of cells. Cell Freezing Medium A (2X) works with Cell Freezing Medium B (2X). Recommendation Suspend…

Sodium acetate is a widely used reagent in molecular biology applications. It is used as a buffer in conjunction with acetic acid, in the buffering range of pH 3.6 – 5.6. Applications Sodium acetate is used in the purification and precipitation of nucleic acids, protein crystallization,…

Alpha MEM is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Recommendation This product is made with Earle’s salts. MEM a contains no proteins, lipids, or…

Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase. The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield. Autoclave sterilized Ready to use Applications …

Sodium Acetate, 3M pH 5.2 is a common molecular biology reagent. All production lots are tested to ensure that they are free of Dnase, Rnase & Proteases. It is often used in buffer systems to maintain the pH of a given solution. Filtered through a 0.22 µm membrane Dnase, Rnase, and Protease…

E199 or Medium 199 with Earle's Salt is one of the best known media in the history of cell culture. Morgan, Morton and Parker's Medium 199 was the first really successful attempt to develop a totally defined medium. Medium 199 was originally developed for nutritional studies of chick embryo…

Eagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…