Cayman Chemical, Chemicals Assay Kits
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Cayman Practice ELISA Kit
Cayman ChemicalThis assay has been developed for researchers that do not have experience performing ELISAs. It can be used as a practice tool allowing you to become comfortable with running Cayman ELISAs. This kit contains enough reagents to run at least four complete standard curves.
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Catalase Assay Kit
Cayman ChemicalCatalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of…
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AR (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of full-length, unmodified human androgen receptor (NR3C4), a ligand-dependent transcription factor commonly referred to as AR. INDIGO’s reporter cells…
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Sampling Tubes with BHT
Cayman ChemicalBHT is a phenolic antioxidant and free radical scavenger. It has been shown to inhibit lipid peroxidation.
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Protein Synthesis Assay Kit
Cayman ChemicalCayman’s Protein Synthesis Assay Kit is a non-radioactive method for measuring protein synthesis that employs the cell-permeable, alkyne-containing, puromycin analog O-Propargyl-puromycin (OPP). Upon application to cells, the OPP probe incorporates into the C-terminus of translating…
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Glutathione Assay Kit
Cayman ChemicalCayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative…
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Anti-Citrullinated Human Fibrinogen Assay Kit (mouse)
Cayman ChemicalImmunization of mice with citrullinated human fibrinogen, especially mice expressing the human HLA-DR4 transgene, induces an arthritic response driven by the production of antibodies that recognize citrullinated epitopes. The polyclonal antibody response produces antibodies reactive with both…
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Acylated Ghrelin (mouse, rat) Easy Sampling EIA Kit
Cayman ChemicalFor the measurement of acylated ghrelin.
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ERß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the full-length human estrogen receptor 2 (NR3A2), a ligand-dependent transcription factor commonly referred to as ERβ. INDIGO’s reporter cells include the…
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RARß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, beta (NR1B2), a ligand-dependent transcription factor commonly referred to as RARB or RARβ. INDIGO’s reporter cells…
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Aconitase Assay Kit
Cayman ChemicalAconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated…
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Aldehyde Dehydrogenase Activity Assay Kit
Cayman ChemicalAldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological functions. Pharmacological inhibitors or activators of ALDH activity have been…
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Myeloperoxidase Peroxidation Fluorometric Assay Kit
Cayman ChemicalCayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and…
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RARy (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor gamma (NR1B3), a ligand-dependent transcription factor commonly referred to as RARG or RARγ. INDIGO’s reporter cells…
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Oxytocin ELISA Kit
Cayman ChemicalOxytocin is a hypothalamic peptide hormone that is stored in the posterior pituitary gland. It is released into the bloodstream during parturition and lactation and is also involved in other social and sexual behaviors, neuropsyciatric disorders, and the maintenance of water and sodium homeostasis.…
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Alanine Transaminase Colorimetric Activity Assay Kit
Cayman ChemicalALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and…
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RXRß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoid X receptor beta (NR2B2), a ligand-dependent transcription factor commonly referred to as RXRB or RXRβ. INDIGO’s reporter cells include…
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Mouse IgG ELISA Kit
Cayman ChemicalImmunoglobulin IgG provides the majority of antibody-based immunity against invading pathogens and plays a significant role in the pathology of auto-immune diseases. Most mouse monoclonal antibodies are of the IgG isotype. Because mice are widely used as a research model for studies of the immune…
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MitoCheck® Complex II/III Activity Assay Kit
Cayman ChemicalComplex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration, and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form…
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Creatinine (serum) Colorimetric Assay Kit
Cayman ChemicalCreatinine is commonly measured in urine and is a key benchmark for the normalization of a variety of urinary biomarkers. Serum creatinine levels, however, are a useful indicator of renal function and abnormal levels have been implicated in diabetes, cardiovascular, and circulatory diseases.…
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TRß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human thyroid hormone receptor beta (NR1A2), a ligand-dependent transcription factor commonly referred to as TRβ. INDIGO’s reporter cells include the…
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WST-1 Cell Proliferation Assay Kit
Cayman ChemicalCayman’s WST-1 Proliferation Assay is based on the reduction of tetrazolium salt WST-1 to a soluble formazan dye by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation.
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LXRß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of human liver X receptor beta (NR1H2), a ligand-dependent transcription factor commonly referred to as LXRβ. INDIGO’s reporter cells include the…
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VDR (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO’s reporter cells include the luciferase reporter…
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Glucose Colorimetric Assay Kit
Cayman ChemicalCayman’s Glucose Assay provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to d-gluconolactone…
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TBARS Assay Kit
Cayman ChemicalOxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically…
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TBARS (TCA Method) Assay Kit
Cayman ChemicalLipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds, which…
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Phagocytosis Assay Kit (IgG PE)
Cayman ChemicalCayman’s Phagocytosis Assay Kit (IgG PE) employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluorescent beads can be detected using a fluorescence microscope, allowing kinetic studies of phagocytosis…
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Nitrate/Nitrite Colorimetric Assay Kit (LDH method)
Cayman ChemicalNADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a…
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Sampling Tubes with Indomethacin
Cayman ChemicalIndomethacin is a COX inhibitor that prevents ex vivo TXB2 formation and other PGs that can not be prevented using anticoagulant alone. The samples have to be transferred in indomethacin sampling tubes as soon as possible after blood collection.
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Progesterone ELISA Kit
Cayman ChemicalProgesterone, along with pregnenolone, is the biosynthetic precursor of all other steroid hormones. The main function of progesterone is to prepare the uterine lining for implantation of a fertilized ovum and to maintain pregnancy. Measurement of serum or plasma progesterone levels are used as an…