Cayman Chemical, Chemicals Assay Kits
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MitoCheck® Complex II Activity Assay Kit
Cayman ChemicalComplex II (succinate dehydrogenase/co-enzyme Q reductase) is one of the major sites of electron entry into the mitochondrial electron transport chain (ETC). Complex II catalyzes the oxidation of succinate to fumarate, and in the process reduces ubiquinone (Q) to ubiquinol (QH2). Ultimately,…
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Aldehyde Dehydrogenase Activity Assay Kit
Cayman ChemicalAldehyde dehydrogenases (ALDHs) represent a group of enzymes that oxidize a wide range of endogenous and exogenous aldehydes to their corresponding carboxylic acids, which affect both developmental and toxicological functions. Pharmacological inhibitors or activators of ALDH activity have been…
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ß-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
Cayman Chemicalβ-HB is a "ketone body" which is produced in the liver, mainly from the oxidation of fatty acids, and is exported to peripheral tissues for use as an energy source. Normally ketosis can indicate that lipid metabolism has been activated and the pathway of lipid degradation is intact.…
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Catalase Assay Kit
Cayman ChemicalCatalase is an ubiquitous antioxidant enzyme that is present in most aerobic cells. It is involved in the detoxification of H2O2, a ROS that is a toxic product of both normal aerobic metabolism and pathogenic ROS production. Cayman’s Catalase Assay utilizes the peroxidatic function of…
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RARß (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, beta (NR1B2), a ligand-dependent transcription factor commonly referred to as RARB or RARβ. INDIGO’s reporter cells…
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WST-1 Cell Proliferation Assay Kit
Cayman ChemicalCayman’s WST-1 Proliferation Assay is based on the reduction of tetrazolium salt WST-1 to a soluble formazan dye by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation.
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Oxytocin ELISA Kit
Cayman ChemicalOxytocin is a hypothalamic peptide hormone that is stored in the posterior pituitary gland. It is released into the bloodstream during parturition and lactation and is also involved in other social and sexual behaviors, neuropsyciatric disorders, and the maintenance of water and sodium homeostasis.…
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CGRP (rat) EIA Kit
Cayman ChemicalCGRP is a 37 amino acid peptide synthesized in the central and peripheral nervous system from a calcitonin/CGRP gene complex. Two isoforms have been described which differ by three amino acids and display similar biological activities: CGRP-α, which is produced by alternative splicing of a…
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SIRT3 Direct Fluorescent Screening Assay Kit
Cayman ChemicalThe sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. Cayman’s Direct Fluorescent Screening Assay Kits provide a convenient…
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Cholesterol Uptake Cell-Based Assay Kit
Cayman ChemicalMaintaining body cholesterol homeostasis is critical for normal physiological functions and is achieved by a highly regulated balance of de novo synthesis, dietary cholesterol absorption, biliary clearance, and excretion. The mechanisms controlling cholesterol absorption are important to understand…
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Mouse IgG ELISA Kit
Cayman ChemicalImmunoglobulin IgG provides the majority of antibody-based immunity against invading pathogens and plays a significant role in the pathology of auto-immune diseases. Most mouse monoclonal antibodies are of the IgG isotype. Because mice are widely used as a research model for studies of the immune…
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RARa (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor, alpha (NR1B1), a ligand-dependent transcription factor commonly referred to as RARA or RARα. INDIGO’s reporter cells…
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Cyclic AMP ELISA Kit (without Acetic Anhydride)
Cayman ChemicalcAMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase…
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RARy (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary non-human cells engineered to provide constitutive, high-level expression of the human retinoic acid receptor gamma (NR1B3), a ligand-dependent transcription factor commonly referred to as RARG or RARγ. INDIGO’s reporter cells…
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Sampling Tubes with BHT
Cayman ChemicalBHT is a phenolic antioxidant and free radical scavenger. It has been shown to inhibit lipid peroxidation.
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Creatinine (urinary) Colorimetric Assay Kit
Cayman ChemicalUrinary creatinine levels are commonly used as an index of standardization for a variety of other tests. Measurement of creatinine clearance is also useful in detecting renal disease and estimating the extent of impairment of renal function. The Cayman Chemical Creatinine (urinary) Colorimetric…
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Pyruvate Assay Kit
Cayman ChemicalPyruvate (pyruvic acid) is a key intermediate in cellular metabolic pathways and is derived primarily from glucose via glycolysis. Abnormal blood pyruvate levels are reported in a number of disorders including shock, liver disease, congestive heart failure, diabetes mellitus, thiamine deficiency,…
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Nitrate/Nitrite Colorimetric Assay Kit (LDH method)
Cayman ChemicalNADPH is an essential cofactor for the function of the NOS enzyme. Unfortunately, NADPH interferes with the chemistry of the Griess reagents, which are the most commonly used reagents for nitrite detection. This kit uses Lactate Dehydrogenase (LDH) to oxidize the excess NADPH used in a…
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Alanine Transaminase Colorimetric Activity Assay Kit
Cayman ChemicalALT is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s Alanine Transaminase Assay provides a convenient method of detecting ALT activity in serum, plasma, tissue samples, and…
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MTT Cell Proliferation Assay Kit
Cayman ChemicalCayman’s MTT Proliferation Assay provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. This kit will also allow investigators to screen drug candidates involved in cell cycle regulation. In this assay, MTT is taken up by cells and…
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S-Nitrosylated Protein Detection Kit (Biotin Switch)
Cayman ChemicalCayman’s S-Nitrosylated Protein Detection Kit (Biotin Switch) employs a modification of the Jaffrey et al. 'Biotin-switch' method to allow for the direct visualization of S-nitrosylated proteins in whole cells or tissues, as well as by WB analysis. Using this method, free SH groups…
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MitoCheck® Complex II/III Activity Assay Kit
Cayman ChemicalComplex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration, and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form…
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ß-Hydroxybutyrate (Ketone Body) Fluorometric Assay Kit
Cayman Chemicalβ-Hydroxybutyrate (β-HB; 3-hydroxybutyric acid) is produced in the liver, mainly from the oxidation of fatty acids, and exported to peripheral tissues for use as an energy source. Measurement of β-HB can provide a reliable index of the level of normal ketosis as well as detect…
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Insulinase Inhibitor Cocktail
Cayman ChemicalFeatures Allows insulin measurement even in hemolyzed samples Contains insulinase inhibitors to protect sample integrity during collection The main analytical pitfalls in insulin measurement in serum are related to (a) hemolysis, (b) circulating anti-insulin autoantibodies, and, for…
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Myeloperoxidase Peroxidation Fluorometric Assay Kit
Cayman ChemicalCayman’s MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and…
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VDR (human) Reporter Assay Kit
Cayman ChemicalThis nuclear receptor assay system utilizes proprietary human cells engineered to provide constitutive, high-level expression of the human vitamin D receptor (NR1I1), a ligand-dependent transcription factor commonly referred to as VDR. INDIGO’s reporter cells include the luciferase reporter…
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Substance P ELISA Kit
Cayman ChemicalSubstance P is a bioactive 11-amino acid peptide first isolated in 1931 from brain and intestine. The peptide is involved in many physiological processes including pain modulation, smooth muscle contraction, blood pressure control, kidney function, and water homeostasis. Substance P is widely…
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XTT Cell Proliferation Assay Kit
Cayman ChemicalCayman’s XTT Cell Proliferation Assay provides an easy to use tool for studying induction and inhibition of cell proliferation in any in vitro model. The assay is based on the extracellular reduction of XTT by NADH produced in the mitochondria via trans-plasma membrane electron transport and…
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Glucose Colorimetric Assay Kit
Cayman ChemicalCayman’s Glucose Assay provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to d-gluconolactone…
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TMRE Mitochondrial Membrane Potential Assay Kit
Cayman ChemicalCayman’s TMRE Mitochondrial Membrane Potential Assay Kit utilizes tetramethylrhodamine ethyl ester (TMRE), a cell permeable, cationic dye which accumulates in the mitochondrial matrix based on mitochondrial membrane potential (ΔψM). This assay, which is suitable for high-throughput…
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Aconitase Assay Kit
Cayman ChemicalAconitase is an iron-sulfur protein containing a [Fe4S4]2+ cluster that catalyzes the stereospecific isomerization of citrate to isocitrate via cis-aconitate. Whereas exposure of aconitase to oxidants renders the enzyme inactive, loss of aconitase activity in cells or in biological samples treated…