Reagents, 41115700
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CDI-activated Separopore® Agarose6B
bioWORLDPre-activated medium for immobilization of ligands containing primary amines N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation No charge is introduced into the matrix after the…
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Protein-A Red Separopore®(Agarose)4B
bioWORLDAffinity matrix with immobilized Protein-A by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Supplied as 25% suspension inPBS with sodium…
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Benzamidine Separopore™ (Agarose)6B
bioWORLDBenzamidine Separopore® 6B is p-aminobenzamidine covalently attached to Separopore ® 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine Separopore ® 6B and…
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Epoxy-activated Separopore®(Agarose)
bioWORLDUsed in coupling of hydroxy, amino or thiol groups to Separopore® 6B via a 12-carbon hydrophilic spacer arm. Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group. Ideal for immobilization of alkali-resistant small ligands such…
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viewIP™ Anti-c-Myc Red Separopore®
bioWORLDRed-Colored Affinity matrix consists of affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to red beaded agarose (Separopore ® ). The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein. Convenient for quantitative capturing of…
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L-Phenylalanine Separopore®(Agarose)
bioWORLDApplication: L-Phenylalanine-Separopore® 4B-CL is used for the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase.
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Amylose Separopore® (Agarose)4B-CL
bioWORLDQuantitative Analysis: Protein extract from E. coli containing a plasmid that expresses a maltose-binding protein-β-galactosidase fusion protein (100,000 units) is passed over a 1 ml column at 4°C. The column is then washed with 10 column volumes of 20 mM Tris-HCl (pH 7.4), 0.2 M…
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Anti-Bovine Albumin (BSA)Red Separo
bioWORLDApplication: For the purification of Bovine Albumin (BSA).
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Red Separopore® 4B-CL
bioWORLDApplications: The immobilized red dye will bind to a wide variety of NADP + - and NAD + -dependent enzymes, including many kinases, dehydrogenases and transferases. Carboxypeptidase G, interferon, plasminogen and plasminogen activator are examples of other proteins that can be purified on…
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Avidin Separopore® (Agarose) 6B
bioWORLDApplications: For the purification of biotin containing proteins.
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Yellow Separopore® (Agarose) 4B-CL
bioWORLDA dye-ligand affinity matrix consists of Reactive yellow-3 bound to cross-linked 4% agarose beads (Separopore® 4B-CL).
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CH Activated Separopore® (Agarose)4B
bioWORLDActivated CH Separopore ® 4B is a pre-activated medieum for covalent immobilization of proteins and other ligands containing primary amino groups.
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Glucose Separopore® (Agarose) 4B-CL
bioWORLDApplications: β-D-Glucose-Separopore® 4B-CL is an agarose conjugate in saline suspension used in affinity chromatography, protein chromatography and carbohydrate matrices. b-D-Glucose-Separopore® 4B-CL has been used in studies to inform pathogenesis of protozoal infections.…
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IgG (Mouse) Separopore® (Agarose)4B
bioWORLDAn affinity chromatography gel matrix consiting of mouse IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic…
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Glycine Separopore® (Agarose) 4B
bioWORLDApplications: Used for the purification of synaptic membrane proteins and other proteins with affinity for glycine
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Blue Separopore® (Agarose) 4B-CL
bioWORLDA dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins. The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® 4B-CL via chlorotriazine ring. Due to a broad spectrum of the affinity, this…
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Goat Anti-Rabbit IgG(H+L)Separopore®
bioWORLDApplication: For use in immunoprecipitation and Co-immunoprecipitation studies.
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L-Aspartic Acid Separopore®(Agarose)
bioWORLDApplications : L-Aspartic Acid-Separopore® 4B-CL is used for the purification of retinol, or other L-Aspartic Acid binding proteins.
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L-Lysine Separopore® (Agarose)4B-CL
bioWORLDApplication: L-Lysine-Separopore® 4B-CL is used for purification of molecules with biospecific or charge dependent affinity for L-lysine. It is also used for the purification of lysine-binding proteins from serum and to purify plasminogen, plasminogen activator, ribosomal RNA, and…
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Brown Separopore® (Agarose) 4B-CL
bioWORLDA dye-ligand affinity matrix consisting of Reactive Brown 10 bound to Cross-linked 4% agarose beads (Separopore® 4B-CL).
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viewIP™ Separopore®
bioWORLDRed-colored agarose matrix contains immobilized Streptavidin for convenient affinity capture (pull-down) of biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands. viewIP TM Red Affinity Gels greatly reduce the risk of pellet loss.…
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Glutathione Separopore® Super Flow
bioWORLDUsed for protein purification by batch/column and scale-up procedures. Glutathione Separopore ® Super Flow (S F) has an exellent protein binding capacity. The good flow properties of the 92 μm (average size) highly cross-linked Separopore ® matrix makes an…
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Biotin Separopore® (Agarose) 6B-CL
bioWORLDApplication: For the purification of avidin and streptavidin containing proteins.
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1 grams make about 10ml gel Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag 1ml drained gel binds 5-10mg of recombinant GS-protein
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GTP Separopore®
bioWORLDApplications: GTP-Separopore® is used for purification of GTP-dependent enzymes and proteins that bind nucleotides and related molecules. The GTP-Separopore® 4B-CL resin comprises GTP attached to agarose beads via its γ-phosphate. A long hydrophilic spacer (14-atom) is used to…
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Poly-L-Lysine Separopore® Ag. 4B-CL
bioWORLDApplication: Poly-L-Lysine-Separopore® 4B-CL is used for the purification of negatively charged proteins and biomolecules.
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D-Alanine Separopore® (Agarose)4B-CL
bioWORLDApplications : Used for the purification of liposomal proteins and glycopeptide antibiotics such as teicoplanin and vancomycin group.
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Phenyl Separopore® (Agarose) 4B-CL
bioWORLDApplications: Phenyl-Separopore® 4B-CL is used for hydrophobic interaction chromatography to purify membrane proteins and other proteins with hydrophobic regions. This matrix separates proteins based on the basis of the different hydrophobicities of the proteins.
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Glutathione Separopore® High Res
bioWORLDGlutathione Separopore® HR is a high resolution matrix highly cross-linked 38 µm agarose bead (6%) matrix.Glutathione Separopore® HR ensures the lowest sample dilution and best high-resolution separation.
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The method involves alkylation of the parent nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N 6 - carboxymethyl derivative with ubsequent condensation using 1,6 diaminohexane to give N 6 -[(6-aminohexyl)carbamoylmethyl)]. Specifications: …