The method involves alkylation of the parent nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6- carboxymethyl derivative with ubsequent condensation using 1,6 diaminohexane to give N6-[(6-aminohexyl)carbamoylmethyl)].
Specifications:
- Lyophilized powder
- 1g yields 3 -5 ml of gel
- Matrix: Highly cross-linked agarose beads, 4%
- Matrix attachment: N6
- Spacer arm: 1, 6 diaminohexane
- Ligand density: 2umol AMP/ml drained medium
- pH stability: 4-10
- Max Flow Rate: 75cm/h.
- Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents Physical stability: Negligible volume variation due to changes in pH or ionic strength