NEW! Protein Expression, 1 mL
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Protein-A Gold™
bioWORLDProtein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus . It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules,…
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Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides…
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Heparin Separopore® 4B is an affinity matrix widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, DNA binding proteins (transcription factors, virus coat proteins etc.) and steroid receptors. It can also be used as a high…
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To remove non-specific binding of proteins to magnetic bead surface and to achieve clean co-immunoprecipitation of your desired antibody or any other ligand binding. Use it with any magnetic beads experiment. Size: 2-5µm. Silica-coated magnetic beads v pH stability: 4-9 Supplied as…
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MagneZoom™A Protein-A Magnetic Beads
bioWORLDRecombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants. Particle size: 2-5µm Ligand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beads pH stability: 4-9 …
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MagneZoom™ CAM (Calmodulin)
bioWORLDCalmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …
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MagneZoom™-PNA (Peanut Agglutini)
bioWORLDMagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…
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bio-World provides immobilized E. coli proteins on a solid bio-affinity support for the removal of antibodies to E. coliproteins from antisera prepared against recombinant proteins expressed in E. coli. Our immobilized E. coli lysate consists of partially purified proteins from E. coli strains…
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Used for purification of anti-IgG antibodies or to purify Protein A. An affinity chromatography gel matrix consisting of rabbit IgG bound to 4% agarose beads (Separopore® 4B). This matrix is coupled through a stable, leak resistant linkage over a wide pH range. May also be used to immunoprecipitate…
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Protease Inhibitor Cocktail
bioWORLDAEBSF E-64 Aprotinin EDTA Leupeptine Store -20°C Lyophillzed blend of five protease inhibitors for applications that require animal free reagents. Reconstitute with 1 ml water to produce a 100X concentrated solution. * Contains: AEBSF Aprotinin (recombinant) E-64 EDTA Leupeptin …
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Protein G binds specifically to Fc regions of many mammalian immunoglobulins Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological…
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The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an…
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Streptavidin Separopore® (Agarose)
bioWORLDApplications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…
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Goat anti-Human IgG Nano-activeGold™
bioWORLDNano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™ reagents consist of affinity-purified IgG antibodies, goat anti-human IgG covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron…
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Anti-biotin Goat IgG Nano-activeGold
bioWORLDNano-activeGold™ can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. Smallest commercially available gold immunoprobes. Penetrates and reaches antigens inaccessible to other probes. Extremely uniform 1.5 nm diameter gold label. …
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Calmodulin-Separopore® 4B is used for affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag. Calmodulin binds proteins principally through their interactions with…
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MagneZoom™, G (Protein G)
bioWORLDRecombinant protein G covalently linked to MagneZoom (paramagnetic beads). Protein G binds Rc region of mammalian immunoglobulin. Ideal for purification and fractionation of IgG subclasses that poorly bind protein A. Suitable for high throughput screening. Particle size: 2-5µm Ligand:…
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Wheat germ -Triticum vulgaris- agglutinin (WGA) linked to MagneZoom (magnetic beads). For magnetic separation by binding specifically to cell particles, glycoconjugates and polysachharides containing N-acetylglucosamine residues. Size: 2-5µm. Ligand: Wheat germ agglutinin Ligand…
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Mixture of six protease inhibitors for use with mammalian cells and tissue extracts. Supplied in l ml DMSO. Will treat proteases extracted from 20g tissue. Contains: AEBSF, E-64, Aprotinin, Leupeptin, Bestatin, Pepstatin A Specificity: Serine Proteases, Cysteine Proteases, Trypsin like…
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Streptavidin- Nano-activeGold™
bioWORLDNano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™ reagent consist of Streptavidin covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. …
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Binding/Wash Buffer Elution Buffer Neutralization Buffer 1X SDS Sample Buffer This kit requires MagneZoom paramagnetic bead station (20160000-1) for separation of beads.
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The BIO-WORLD Abundant Protein Removal-Haptoglobin kit removes the high-abundant protein- Haptoglobin from human serum and plasma. The high affinity-based column contains separopore-matrix coupled with antibodies against the serum protein. The presence of abundant proteins in human serum and…
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The bio-World Abundant Protein Removal-HSA kit removes the high-abundant protein- HSA from human serum and plasma. The high affinity-based column contains Separopore®-matrix coupled with antibodies against the serum protein. The presence of abundant proteins in human serum and plasma often…
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MagneZoom Protein G (superparamagnetic beads) of average 5 µm in diameter, covalently coated with recombinant Protein G. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains…
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The bio-World Abundant Protein Removal a-1-antitrypsin kit removes the high-abundant protein a-1-antitrypsin from human serum and plasma. The high affinity-based column contains Separopore® matrix coupled with antibodies against a-1-antitrypsin. The presence of abundant proteins in human…
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Anti-Bovine Albumin BSA Separopore®
bioWORLDApplication: Anti-BSA-Separopore® 4B is used for immunoprecipitation and immunoaffinity purification of bovine serum albumin (BSA) from solutions (and co-immunoprecipitation of biomolecules that bind BSA). It is also used to remove BSA from solutions.
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Mixture of three protease inhibitors with specificity for inhibiting cysteine proteases. Supplied in lm DMSO. Contains: ALLN, Antipain, E-64 Specificity: Calpain l/ll, Cathepsin A/B, Cathepsin L/S Papain, and Cysteine Proteases This product is sold for laboratory research use only.
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Streptavidin covalently linked to MagneZoom™ (magnetic beads). For magnetic separation and isolation of biotinylated ligands for affinity separations. Used in high throughput screening. Surface silica coated magnetic beads Bead Size: 3-5 µm Supplied as suspension (25mg/ml) in phosphate…
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Mixture of four protease inhibitors for inhibiting serine proteases. Supplied in lml DMSO. Contains: AEBSF, Aprotinin, Elastatinal, GGACK Specificity: Chymotrypsin, Kallikrein, Plasmin Thrombin, Trypsin, Elastase, Urokinase and Factor Xa Usually ships within 24 hours. This…
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Mixture of six protease inhibitors for use with plant cell extracts. Supplied in lml DMSO. Will inhibit proteases extracted from 30g plant tissue. Contains: AEBSF, Bestatin, E-64, Leupeptin, Pepstatin A, Phenanthroline Specificity: Serine Proteases, Aminopeptidases, Cysteine Proteases,…
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Mixture of four protease inhibitors for use with fungal and yeast extracts. Supplied in l ml DMSO. Will inhibit proteases extracted from 20g yeast. Contains: AEBSF ,Pepstatin A, E-64, Phenanthroline Specificity: Serine Protease, Cysteine Proteases, Aspartic Proteases and Metalloproteases …