NEW! Protein Expression, bioWORLD

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bioWORLD NEW! Protein Expression

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  • Calmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …

  • bioWORLD

    Cu-immobilized metal affinity chromatography (IMAC) columns consist of Cu-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Cu-IMAC Columns separate proteins via their differences in affinity to Cu2+ metal ions. Ideal for purification of recombinant…

  • MagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…

  • Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…

  • Silica-coated uniform superparamagnetic beads, MagneZoom, with an aldehyde surface group. It is widely used to covalently couple ligands containing primary and secondary amino groups. This coupling method will provide an extremely stable linkage between the ligand and the matrix. Size: 2-5µm …

  • Bioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…

  • Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant…

  • Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • Supplied as a 2.5% suspension MagneZoom™-Epoxy are silica-coated super paramagnetic beads with an Epoxy surface group. bioWORLD’s MagneZooms are suitable for high throughput screening. Matrix: Silica coated magnetic beads. Magnetic bead ligand: Epoxy. Particle size:…

  • bioWorld's Carboxylated 96-well Microplates contains a COOH-coated surface to which the amino group present in any molecules, such as peptides or proteins, binds to through formation of amide bonds by the action of carbodiimide. Applications: Coupling molecules, having an amino group, to…

  • Poly-Lysine coated microplates High quality coated microplates. The Poly-Lysine coating of these 96-well microplates offers specific, high affinity binding to a variety of cell types.

  • bioWORLD

    A biotin-binding glycoprotein prepared from chicken egg white, Molecular weight=67kDa, pI = 10 Glycosylation and isoelectric point make avidin more soluble than streptavidin Significantly less expensive than recombinant streptavidin protein Based on strong binding with biotin, avidin is…

  • Functions by binding to hemoglobin. 98.0% (HPCE), powder, color off-white Occurs as 3 major phenotypes: type 1-1, type 2-1, type 2-2

  • Streptavidin Coated Plates are available in 96-well plate format, and pre-blocked with BSA   Blocking Buffer. bio-world Biotin-Binding Plates are available in clear to be used with colorimetricdetection methods. Easy and gentle immobilization of biotin-containing conjugates…

  • Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasses. Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants. Provided as pre-packed,…

  • From Streptomyces avidini Biotechnology Grade Activity: >17U/mg protein Electrophoresis: Pass DNase and RNase Activity: None detected Protease: None detected Product for in vitro and lab research use only, not for human use or consumption

  • The presence of abundant proteins in human serum and plasma often masks the detection of low-abundant proteins of interest that serve as biomarkers for disease when being studied by 2D gel electrophoresis or LC/MS. After depletion of these high-abundant proteins, serum samples consist of an…

  • Recombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants. Particle size: 2-5µm Ligand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beads pH stability: 4-9 …

  • To remove non-specific binding of proteins to magnetic bead surface and to achieve clean co-immunoprecipitation of your desired antibody or any other ligand binding. Use it with any magnetic beads experiment. Size: 2-5µm. Silica-coated magnetic beads v pH stability: 4-9 Supplied as…

  • bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

  • Biotin Coated Plates can be used as the basis for a variety of biomolecular assay techniques requiring the immobilization of probes that are conjugated with NeutrAvidin Protein, streptavidin, avidin or other biotin-binding proteins. Specifications: Biotin group accessible for binding…

  • Protein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus . It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules,…

  • Protein quantification is often required before precessing protein samples for analysis.The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+to Cu1+by proteins in an alkaline…

  • An affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP).  Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times.  Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…

  • Reagent grade, fatty acid free Moisture: < 1.0% Heavy metals: <0.5% Endotoxin: ≤0.1EU/mg pH (10% water): 6.5 - 7.5

  • 1mg/ml BSA solution in 0.85% Sodium Chloride and 0.01% Sodium azide.

  • The bioLifeTM MUG ß-Galactosidase Assay Kit provides a highly sensitive fluorogenic subsatrate for detection of ß-galactosidase activity in tranfected cells. In the presence of ß-galactosidase the colorless 4-methylumbelliferyl B-D-galactopyranoside (MUG) substrate is hydrolyzed…

  • Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…

  • Mixture of six protease inhibitors for use with mammalian cells and tissue extracts. Supplied in l ml DMSO. Will treat proteases extracted from 20g tissue. Contains: AEBSF, E-64, Aprotinin, Leupeptin, Bestatin, Pepstatin A Specificity: Serine Proteases, Cysteine Proteases, Trypsin like…

  • Nano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™  reagent consist of Streptavidin covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron microscopy to provide clear visibility. …

  • Applications: Reduced glutathione is covalently linked Separopore® 4B for use in affinity purification of glutathione-S-transferase (GST) and GST-fused proteins. This product provides a one step purification method and permits rapid, mild and highly selective purifications of proteins…

  • Supplied as a 2.5% suspension MagneZoom™-COOH are silica-coated super paramagnetic beads with an COOH surface group. bioWORLD’s MagneTons are suitable for high throughput screening Matrix: Silica coated magnetic beads. Magnetic bead ligand: COOH. Particle size: 1-2µm. …

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