NEW! Protein Expression, bioWORLD

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bioWORLD NEW! Protein Expression

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  • Streptavidin Coated Plates are available in 96-well plate format, and pre-blocked with BSA   Blocking Buffer. bio-world Biotin-Binding Plates are available in clear to be used with colorimetricdetection methods. Easy and gentle immobilization of biotin-containing conjugates…

  • Bioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…

  • Protein A coated microplates provide alternative methods for immobilizing antibodies for ELISA and other plate-based assay techniques. These plates are uniformly and stably coated with Protein A. They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of…

  • Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…

  • Bioalginate beads Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent…

  • Macro-spin columns have a packing volume of 500 μl suitable for centrifugation of 50-150 μl sample volume. Prepacked with Sephadex G-50 Collect DNA with small MW (10-20 bases) Suitable for use with Swing-out Rotor Centrifuge No High Speed needed avg, speed needed is 1000-2000…

  • Jacalin, belonging to the lectins family, is a Hemagglutinin from the seeds of the jack fruit Artrocarpus integrifolia . It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures. Jacalin shows specific…

  • Zerobind™ affinity columns are designed to remove non-specific backgrounds from various polyclonal antibodies generally used for western blotting. Passing the diluted Antibody solution through Zerobind™ column can reduce non-specific binding while retaining activity against your…

  • MagneZoom-PNA are silica-coated super paramagnetic beads covalently linked to Peanut Agglutinin (PNA). Peanut agglutinin is a 110 kDa homotetrameric, non-glycosylated lectin with a high binding affinity for the tumor associated Thomsen-Friedenreich antigen (“T’-antigen) and binds preferentially to…

  • Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10…

  • Wheat germ lectin (Isolated from Triticum vulgaris) conjugated to BlackPearl® Magnetic Beads. Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor…

  • Nano-activeGold™ is a newly developed gold immunoprobe. Nano-ActiveGold™  reagents consist of affinity-purified IgG antibodies, goat anti-human IgG covalently conjugated to the 1.5 nm Nano-activeGold™ cluster label. Nano-activeGold™ can be used in immunoblotting, light microscopy, and electron…

  • bioWold's Streptalon (Streptavidin magnetic beads) Kit is designed for convenient and quick purification of biotinylated proteins and nucleic acids in immunoprecipitation, protein interaction studies, immunoassays and cell isolation, etc. Streptalon (Streptavidin Magnetic Beads) Kit…

  • Buffered fish gelatin 0.05% blocks, diluents and coating solutions are supplied as 10X concentrates. Sold as 2 x 100mL or 500mL. pH range: 8.0 (+/- 0.1) formulated in borate buffered physiologic saline buffer containing glycerol as a preservative

  • Protein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus . It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules,…

  • bio-World's Carboxy Coupling Kit consists of amino-terminated Separopore® 4B-CL (4% crosslinked agarose beads). This kit is suitable for covalent immobilization of peptides or other carboxyl-containing (–COOH) molecules for use in affinity purification procedures. It includes carboxyl Coupling…

  • The bioLifeTM MUG ß-Galactosidase Assay Kit provides a highly sensitive fluorogenic subsatrate for detection of ß-galactosidase activity in tranfected cells. In the presence of ß-galactosidase the colorless 4-methylumbelliferyl B-D-galactopyranoside (MUG) substrate is hydrolyzed…

  • AEBSF E-64 Aprotinin EDTA Leupeptine Store -20°C Lyophillzed blend of five protease inhibitors for applications that require animal free reagents. Reconstitute with 1 ml water to produce a 100X concentrated solution. * Contains: AEBSF Aprotinin (recombinant) E-64 EDTA Leupeptin …

  • Calmodulin covalently bound to MagneZoom magnetic beads. For purification of calmodulin-binding proteins and calmodulin-regulated proteins. Proteins containing a calmodulin binding peptide fusion tag can also be purified using MagneZoom - CAM. Particle Size: 2-5µm Ligand: Bovine Calmodulin …

  • Recombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants. Particle size: 2-5µm Ligand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beads pH stability: 4-9 …

  • Erythrina cristagalli (coral tree) lectin is a 54,000 dalton glycoprotein consisting of two different subunits of approximately 28,000 and 26,000 daltons. It has a specificity toward galactose residues and appears to have the highest binding activity toward galactosyl ( b -1,4)…

  • Applications: Affinity matrix with immobilized Streptavidin by CNBr activation coupling method. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated…

  • Poly-Lysine coated microplates High quality coated microplates. The Poly-Lysine coating of these 96-well microplates offers specific, high affinity binding to a variety of cell types.

  • bioWORLD

    A biotin-binding glycoprotein prepared from chicken egg white, Molecular weight=67kDa, pI = 10 Glycosylation and isoelectric point make avidin more soluble than streptavidin Significantly less expensive than recombinant streptavidin protein Based on strong binding with biotin, avidin is…

  • bioSilica Beads Non-porous silica beads are useful in molecular biology and immunoassays. Diameters are 0.3-5.0 µm, a density of 1.96g/cm 3 , a refractive index (589nm) of about 1.37. Silica beads have high temperature stability. Also, because hydrophilic silica does not absorb proteins,…

  • Bioalginate Alginate, commonly called sodium alginate, is a linear polysaccharide. The copolymer consists of two uronic acids: D-mannuronic acid (M) and L-guluronic acid (G ). Sodium alginate as well as other alkali metal alginates are water soluble, whereas the salts of polyvalent cations,…

  • Source: E.Coli Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ~3…

  • Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions. Properties Affinity matrix for proteins. Imminodiacetate immobilized matrix charged with Co metal…

  • Functions by binding to hemoglobin. 98.0% (HPCE), powder, color off-white Occurs as 3 major phenotypes: type 1-1, type 2-1, type 2-2

  • Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant…

  • Quick Concentrators™ for proteins. Prepacked columns of Q-Cons™, just add diluted proetein and spin, then collect concentrated sample. Concentrate proteins 5 to 20 fold in 5 minutes. 5ml column Concentrates 4 ml volume

  • An affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP).  Amylose is linked to Separopore® (Agarose) 4B-CL. The resin may be generated up to five times.  Store used resin in column buffer containing 0.02% sodium azide. Quantitative Analysis: Protein…

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