Viral Gene Delivery
-
CMV-MAD2 lentivirus
Cellomics TechnologyMAD2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2 was showed to be associated with tumor differentation. This lentivirus express human MAD2 under the control of the CMV promoter.
-
ANG shRNA lentivirus
Cellomics TechnologyANG gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is regulated by…
-
Inducible tet-on Ars2 lentivirus
Cellomics TechnologyArs2 (arsenite-resistance protein 2; also known as Srrt) is a highly conserved zinc finger protein. Ars2 is necessary and sufficient to promote NSC (neural stem cells) self-renewal. This premade lentivirus expresses Ars2 under the control of Tet promoter.
-
CMV-OTX2 lentivirus
Cellomics TechnologyOTX2 is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. This lentivirus expresses human OTX2 under the control of the CMV promoter.
-
The ViraTrap™ retrovirus mini purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 3 x10 12 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the…
-
Inducible tet-on Chx10 lentivirus
Cellomics TechnologyThe homeodomain transcription factor Chx10 is one of the earliest markers of the developing retina. It is required for retinal progenitor cell proliferation as well as formation of bipolar cells, a type of retinal interneuron.
-
Inducible tet-on miR-183 lentivirus
Cellomics TechnologyIt has been observed that microRNA miR-183 plays an important role in tumorigenesis and cancer metastasis. This lentivirus expresses miR-183 under the control of the Tet promoter.
-
Inducible tet-on OCT3/4 lentivirus
Cellomics TechnologyOCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…
-
CMV-RAX lentivirus
Cellomics TechnologyRAX (Retina And Anterior Neural Fold Homeobox) is a homeobox-containing transcription factor that functions in eye development. RAX is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in…
-
heat inducible GFP lentivirus
Cellomics TechnologyGreen fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…
-
Set of lentivirus (CMV-driven SOX2, OCT3/4, KLF4 and C-MYC)
Cellomics TechnologyThis set contains four CMV-driven vectors: SOX2 (PLV-10008), OCT3/4 (PLV-10007), KLF4 (PLV-10006) and C-MYC (PLV-10005).
-
CMV-LHX2 lentivirus
Cellomics TechnologyLHX2 (LIM Homeobox 2) belongs to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. LHX2 is involved in neuronal differentiation. This lentivirus expresses human LHX2 under the control of the CMV promoter.
-
Inducible tet-on MITF-D lentivirus (Bsd)
Cellomics TechnologyMicrophthalmia-associated transcription factor (MITF) is a member of the basic/helix-loop-helix/leucine zipper (b-HLH-ZIP) transcription factor subfamily. MITF regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived…
-
EF1A-C-MYC lentivirus
Cellomics TechnologyMYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin…
-
Inducible tet-on NEUROD1 lentivirus
Cellomics TechnologyNEUROD1 is a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and…
-
ADAM-17 shRNA lentivirus
Cellomics TechnologyADAM-17 gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM-17 is an…
-
PGK-Cre, CMV-Renilla luciferase lentivirus
Cellomics TechnologyCre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…
-
Inducible tet-on NR2E3 lentivirus
Cellomics TechnologyNR2E3, a photoreceptor-specific orphan nuclear receptor, is believed to play a pivotal role in the differentiation of photoreceptors. Mutations in NR2E3 result in syndromes characterized by excess blue cones and loss of rods: enhanced S-cone syndrome (ESCS). This lentivirus expresses human NR2E3…
-
Set of lentivirus (EF1A-driven LIN28, NANOG, SOX2, OCT3/4, KLF4 and C-MYC)
Cellomics TechnologyThis set contains six EF1A-driven vectors: LIN28 (PLV-10015), NANOG (PLV-10014), SOX2 (PLV-10013), OCT3/4 (PLV-10012), KLF4 (PLV-10011) and C-MYC (PLV-10010). Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in…
-
Calcium indicator GCaMP3 lentivirus (CMV)
Cellomics TechnologyCalcium is a universal second messenger regulating essential cellular signaling events in a broad range of tissues and organisms. GCaMP3 is a fusion calcium indicator of green fluorescent protein (GFP), calmodulin and the M13 domain of a myosin light chain kinase designed to show the calcium ion…
-
Inducible tet-on PAX6 lentivirus
Cellomics TechnologyPax6 gene contains a paired domain and a paired-type homeodomain and is expressed during early neuroretina development. Pax6 is required for multipotency of retinal progenitors. Tansfection of Pax6 into ES or iPS cells induces retinal progenitors. Pax6 alone is sufficient to induce…
-
Inducible tet-on MITF-D lentivirus (Puro)
Cellomics TechnologyMicrophthalmia-associated transcription factor (MITF) is a member of the basic/helix-loop-helix/leucine zipper (b-HLH-ZIP) transcription factor subfamily. MITF regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived…
-
PGK-GFP lentivirus
Cellomics TechnologyGreen fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…
-
Angiogenin (Ang) shRNA lentivirus
Cellomics TechnologyAngiogenin (Ang) gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is…
-
CMV-SOX2 lentivirus
Cellomics TechnologySRY (sex determining region Y)-box 2 (SOX2) is a transcription factor critical for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of SOX2 has been used to produce iPS cells in culture.
-
CMV-TNFSF12 lentivirus
Cellomics TechnologyTNFSF12 is a member of the tumor necrosis factor (TNF) ligand family. TNFSF12 is a ligand for the FN14/TWEAKER receptor involved in apoptosis and angiogenesis. This lentivirus expresses human TNFSF12 under the control of the CMV promoter.
-
CMV-LacZ lentivirus
Cellomics TechnologyLacZ catalyzes the hydrolysis of the disaccharide lactose, into gluclose and galactose. The use of LacZ as a reporter gene is due to its ability to cleave the bond between galactose and the indole, 5-bromo-4-chloro-3-hydroxyindole. The cleaved indole, is an easily detectable and insoluble blue compound.
-
Inducible tet-on SOX2 lentivirus
Cellomics TechnologySRY (sex determining region Y)-box 2 (SOX2) is a transcription factor critical for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of SOX2 has been used to produce iPS cells in culture. This lentivirus expresses Sox2 under the control of the Tet promoter.
-
CMV-OSKM lentivirus
Cellomics TechnologyInduced pluripotent stem (iPS) cells can be generated from differentiated somatic cells by over-expressing a set of specific transcription factors called reprogramming factors. Typically four reprogramming factors are chosen, Oct4, Sox2, Klf4 and c-Myc (OSKM), which are introduced into the target…
-
NF-kB Firefly luciferase reporter lentivirus (Puro)
Cellomics TechnologyThis NF-kB reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the NF-kB transcriptional response element (TRE). It can be used to monitor the transcriptional activity of NF-kB.
-
CMV-GFP-IRES-Puro lentivirus
Cellomics TechnologyGreen fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…
-
EF1A-LIN28 lentivirus
Cellomics TechnologyLIN28 is a microRNA-binding protein that has been shown to bind the let-7 pre-microRNA and block production of mature let-7 in embryonic stem cells. This activity appears to preserve undifferentiated state of stem cells. Subsequently, LIN28 expression has been used to generate iPS cells. Human…