Viral Gene Delivery
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CAG-RFP lentivirus
Cellomics TechnologyTurboRFP is a red (orange) fluorescent protein (ex. 553 and em. 574 nm) derived from sea anemoneEntacmaea quadricolor [Merzlyak et al., 2007]. Possessing high photostability and pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in…
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BMP4 shRNA lentivirus
Cellomics TechnologyBMP4 gene encodes a protein of the bone morphogenetic protein (BMP) family, which is part of the transforming growth factor-beta (TGF-beta) superfamily. The superfamily includes large families of growth and differentiation factors. Members of the BMP family play an important role in the onset of…
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EF1A-OCT3/4 lentivirus
Cellomics TechnologyOCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…
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CMV-MITF-H lentivirus
Cellomics TechnologyMITF-H is an isoform of MITF (microphthalmia-associated transcription factor), a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible…
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CMV-Cre-GFP lentivirus
Cellomics TechnologyCre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…
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CMV-Pax6 lentivirus
Cellomics TechnologyPax6 (paired box 6) is a transcriptional regulator with paired box domain and homeo box domain. Pax6 is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. This lentivirus expresses…
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Set of lentivirus (Tet-driven SOX2, OCT3/4, KLF4 and C-MYC)
Cellomics TechnologyThis set contains four Tet-driven vectors: KLF4 (PLV-10026), SOX2 (PLV-10027), C-MYC (PLV-10028), and OCT3/4 (PLV-10019).
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Inducible tet-on CRX lentivirus
Cellomics TechnologyCRX is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. CRX play an important role in transdifferentiation of photoreceptor cells. This lentivirus expresses human Crx under the control of the Tet promoter.
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Inducible tet-on Brn2 lentivirus
Cellomics TechnologyBRN2 (also known as POU3F2) encodes a member of the POU-III class of neural transcription factors. The encoded protein is involved in neuronal differentiation and enhances the activation of corticotropin-releasing hormone regulated genes. Overexpression of this protein is associated with an…
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Inducible tet-on Smad6 lentivirus
Cellomics TechnologySmad6 and Smad7 are inhibitory Smads induced by transforming growth factor β-Smad signal transduction pathways in a negative-feedback mechanism. Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube. Smad6 is also involved in multiple tissue differentiation…
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Inducible tet-on OSKM lentivirus
Cellomics TechnologyInduced pluripotent stem (iPS) cells can be generated from differentiated somatic cells by over-expressing a set of specific transcription factors called reprogramming factors. Typically four reprogramming factors are chosen, Oct4, Sox2, Klf4 and c-Myc (OSKM), which are introduced into the target…
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Inducible tet-on miR-204 lentivirus
Cellomics TechnologyIt has been observed that microRNA miR-204 plays a role in the regulation of cancer metastasis. This lentivirus expresses miR-204 under the control of the Tet promoter.
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HPSE shRNA lentivirus
Cellomics TechnologyHeparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix.Heparanase (HPSE) is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. HPSE is critical regulator of angiogenesis and tumor…
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Inducible tet-on C-MYC lentivirus
Cellomics TechnologyMYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture. This lentivirus expresses C-Myc under the control of the Tet promoter.
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CHI3L1 shRNA lentivirus
Cellomics TechnologyIn humans, CHI3L1 encodes Chitinase-3-like protein 1 (CHI3L1) that a glycoprotein member of the glycosyl hydrolase 18 family. It is a secreted glycoprotein and catalyzes the hydrolysis of chitin glycopolymer found in insect exoskeletons and fungal cell walls. The protein lacks chitinase activity…
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ANGPTL4 shRNA lentivirus
Cellomics TechnologyANGPTL4 gene encodes a glycosylated, secreted Angiopoietin-like 4 protein containing a C-terminal fibrinogen domain. The encoded protein plays an critical role in numerous cancers and it is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and…
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CMV-SIX3 lentivirus
Cellomics TechnologySIX3 is a member of the sine oculis homeobox transcription factor family and plays a role in eye development. Mutations in SIX3 have been associated with holoprosencephaly type 2. This lentivirus expresses human SIX3 under the control of the CMV promoter.
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UBC-Cre-RFP lentivirus
Cellomics TechnologyCre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…
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EF1A-HLF lentivirus
Cellomics TechnologyHepatic Leukemia Factor (HLF) is a member of the proline and acidic-rich (PAR) family, a subset of the bZIP transcription factors. Chromosomal translocation of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemia. This lentivirus expresses human HLF under the…
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HPSE shRNA lentivirus
Cellomics TechnologyHeparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. Heparanase (HPSE) is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. HPSE is critical regulator of angiogenesis and…
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CMV-MITF-A lentivirus
Cellomics TechnologyMITF-A is an isoform of MITF (microphthalmia-associated transcription factor), a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible…
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UBC-Firefly luciferase lentivirus (Neo)
Cellomics TechnologyLuciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis. This lentivirus expresses Firefly luciferase under the control of UBC promoter.
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CMV-Renilla luciferase lentivirus
Cellomics TechnologyLuciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Renilla luciferase refers to the enzyme that was isolated from the sea pansy, Renilla reniformis.
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CMV-KLF4 lentivirus
Cellomics TechnologyKrueppel-like factor 4 (KLF4) is a zinc finger transcriptional regulator that has been identified as a good indicator of stem cell-like capacity and is subsequently used in iPS cell generation. KFL4 plays an important role in maintaining stem cells, and in preventing their differentiation.
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CMV-GFP lentivirus
Cellomics TechnologyGreen fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…
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The ViraTrap™ lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Up to 3x10 10 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. The viruses are first…
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Oct4-GFP lentivirus
Cellomics TechnologyIn this lentivirus, the GFP repoter gene is expressed from the Oct4 promoter that is active when cells are in an undifferentiated state. Upon differentiation, the Oct4 promoter is gradually inactivated and therefore the GFP reporter is down-regulated. Oct4-GFP expression is used as a reprogramming marker.
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HIF1A shRNA lentivirus
Cellomics TechnologyHIF1A gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes,…
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Inducible tet-on miR-211 lentivirus
Cellomics TechnologyMicroRNAs (miRNAs) are non-coding RNAs that play roles in gene silencing. miR-211 was mapped to chromosome 15q13, a locus frequently altered in cancers. Studies showed miR-211 is involved in cancer cell growth and cancer metastasis.
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EF1A-KLF4 lentivirus
Cellomics TechnologyKrueppel-like factor 4 (KLF4) is a zinc finger transcriptional regulator that has been identified as a good indicator of stem cell-like capacity and is subsequently used in iPS cell generation. KFL4 plays an important role in maintaining stem cells, and in preventing their differentiation. Human…
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PGK-Cre, CMV-Firefly luciferase lentivirus
Cellomics TechnologyCre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…
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STAT3 Firefly luciferase reporter lentivirus (Puro)
Cellomics TechnologyThis STAT3 reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the STAT3 transcriptional response element (TRE). It can be used to monitor the transcriptional activity of STAT3.