Viral Gene Delivery

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Viral Gene Delivery

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  • Calcium is a universal second messenger regulating essential cellular signaling events in a broad range of tissues and organisms. GCaMP3 is a fusion calcium indicator of green fluorescent protein (GFP), calmodulin and the M13 domain of a myosin light chain kinase designed to show the calcium ion…

  • CMV-LacZ lentivirus

    Cellomics Technology

    LacZ catalyzes the hydrolysis of the disaccharide lactose, into gluclose and galactose. The use of LacZ as a reporter gene is due to its ability to cleave the bond between galactose and the indole, 5-bromo-4-chloro-3-hydroxyindole. The cleaved indole, is an easily detectable and insoluble blue compound.

  • Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis. This lentivirus expresses Firefly luciferase under the control of CMV promoter.

  • CMV-GFP-IRES-Puro lentivirus

    Cellomics Technology

    Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

  • EF1A-RFP lentivirus

    Cellomics Technology

    TurboRFP is a red (orange) fluorescent protein (ex. 553 and em. 574 nm) derived from sea anemoneEntacmaea quadricolor [Merzlyak et al., 2007]. Possessing high photostability and pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in…

  • This STAT3 reporter lentivirus expresses Firefly luciferase under the control of a minimal (m)CMV promoter and tandem repeats of the STAT3 transcriptional response element (TRE). It can be used to monitor the transcriptional activity of STAT3.

  • CMV-GFP lentivirus

    Cellomics Technology

    Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

  • CXCL1 shRNA lentivirus

    Cellomics Technology

    In humans, CXCL1 gene encodes a small chemokine (C-X-C motif) ligand 1 (CXCL1) cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-α). CXCL1 plays…

  • The ViraTrap™ Adenovirus maxi purification kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 4 x10 12 viral particles can be purified from cell culture media of 6 to 8 T75 flasks. Traditionally the…

  • The ViraTrap™ lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column. The viruses are first applied…

  • UBC-Cre-RFP lentivirus

    Cellomics Technology

    Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

  • EF1A-GFP lentivirus

    Cellomics Technology

    Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

  • The ViraTrap™ retrovirus maxi purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 1 x10 13 viral particles can be purified from cell culture media of 6 to 8 T75 flasks. Traditionally the…

  • The ViraTrap™ lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Up to 3x10 10 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. The viruses are first…

  • The ViraTrap™ retrovirus mini purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 3 x10 12 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the…

  • CMV-C-MYC lentivirus

    Cellomics Technology

    MYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture.

  • EF1A-SOX2 lentivirus

    Cellomics Technology

    SRY (sex determining region Y)-box 2 (SOX2) is a transcription factor critical for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of SOX2 has been used to produce iPS cells in culture. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive…

  • CMV-Cre-GFP lentivirus

    Cellomics Technology

    Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

  • TGFß/SMAD reporter lentivirus

    Cellomics Technology

    The TGFβ/SMAD reporter lentivirus is designed for monitoring the activity of the TGFβ/SMAD signaling pathway. The transforming growth factor beta (TGFβ) signaling pathway is involved in many cellular processes in both the adult organism and the developing embryo including cell…

  • ANGPTL4 shRNA lentivirus

    Cellomics Technology

    ANGPTL4 gene encodes a glycosylated, secreted Angiopoietin-like 4 protein containing a C-terminal fibrinogen domain. The encoded protein plays an critical role in numerous cancers and it is implicated in the metastatic process by modulating vascular permeability, cancer cell motility and…

  • ANG shRNA lentivirus

    Cellomics Technology

    ANG gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is regulated by…

  • Angpt1 gene encodes a secreted glycoprotein called Angiopoietin 1 that activates the receptor by inducing its tyrosine phosphorylation. It appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. It may also mediate blood…

  • CMV-MAD2 lentivirus

    Cellomics Technology

    MAD2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2 was showed to be associated with tumor differentation. This lentivirus express human MAD2 under the control of the CMV promoter.

  • Inducible tet-on OCT3/4 lentivirus

    Cellomics Technology

    OCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…

  • ADAM-17 shRNA lentivirus

    Cellomics Technology

    ADAM-17 gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM-17 is an…

  • BMP4 shRNA lentivirus

    Cellomics Technology

    BMP4 gene encodes a protein of the bone morphogenetic protein (BMP) family, which is part of the transforming growth factor-beta (TGF-beta) superfamily. The superfamily includes large families of growth and differentiation factors. Members of the BMP family play an important role in the onset of…

  • Angiogenin (Ang) shRNA lentivirus

    Cellomics Technology

    Angiogenin (Ang) gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is…

  • CHI3L1 shRNA lentivirus

    Cellomics Technology

    In humans, CHI3L1 encodes Chitinase-3-like protein 1 (CHI3L1) that a glycoprotein member of the glycosyl hydrolase 18 family. It is a secreted glycoprotein and catalyzes the hydrolysis of chitin glycopolymer found in insect exoskeletons and fungal cell walls. The protein lacks chitinase activity…

  • CIAPIN1 shRNA lentivirus

    Cellomics Technology

    In humans, CIAPIN1 (Cytokine Induced Apoptosis Inhibitor 1) encodes a protein called Anamorsin. Expression of CIAPIN1 is dependent on growth factor stimulation. It has no relationship to apoptosis regulatory molecules of the BCL2 or CASP families. Its related pathways include Metabolism and…

  • OTX2 is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. This lentivirus expresses human OTX2 under the control of the Tet promoter.

  • Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

  • CMV-MITF-H lentivirus

    Cellomics Technology

    MITF-H is an isoform of MITF (microphthalmia-associated transcription factor), a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible…

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