Hnf4a is a nuclear transcription factor which binds DNA as a homodimer. Hnf4a controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver,…

ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1) is a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding.…

TurboRFP is a red (orange) fluorescent protein (ex. 553 and em. 574 nm) derived from sea anemoneEntacmaea quadricolor [Merzlyak et al., 2007]. Possessing high photostability and pH stability, TurboRFP is more than twice brighter than DsRed2. Fast TurboRFP maturation makes it clearly detectable in…

The ViraTrap™ adenovirus mini purification kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 1 x10 12 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the…

The basic motif-leucine zipper transcription factor NRL (neural retina leucine zipper) is essential for rod photoreceptor specification in the mammalian retina. This lentivirus expresses human Nrl under the control of the Tet promoter.

PR domain containing 1 with zinc finger domain (PRDM1)/B lymphocyte–induced maturation protein 1 (BLIMP1) is a transcriptional repressor. PRDM1 plays fundamentally important roles in many cell lineages and in the early development of several cell types, including B and T lymphocytes, germ…

MAD2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2 was showed to be associated with tumor differentation. This lentivirus express human MAD2 under the control of the CMV promoter.

ANG gene encodes a mediator of new blood vessel formation that hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. It interacts with DNA causing a promoter-like increase in the expression of rRNA. Angiogenic activity is regulated by…

Ars2 (arsenite-resistance protein 2; also known as Srrt) is a highly conserved zinc finger protein. Ars2 is necessary and sufficient to promote NSC (neural stem cells) self-renewal. This premade lentivirus expresses Ars2 under the control of Tet promoter.

The ViraTrap™ lentivirus mini purification kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column. The viruses are first applied…

The ViraTrap™ retrovirus maxi purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 1 x10 13 viral particles can be purified from cell culture media of 6 to 8 T75 flasks. Traditionally the…

OTX2 is a homeodomain transcription factor and one of the key regulator genes of photoreceptor formation. This lentivirus expresses human OTX2 under the control of the CMV promoter.

The ViraTrap™ retrovirus mini purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 3 x10 12 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the…

The homeodomain transcription factor Chx10 is one of the earliest markers of the developing retina. It is required for retinal progenitor cell proliferation as well as formation of bipolar cells, a type of retinal interneuron.

It has been observed that microRNA miR-183 plays an important role in tumorigenesis and cancer metastasis. This lentivirus expresses miR-183 under the control of the Tet promoter.

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

OCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS…

RAX (Retina And Anterior Neural Fold Homeobox) is a homeobox-containing transcription factor that functions in eye development. RAX is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in…

Green fluorescent protein (GFP), a 27 kDa protein derived from the jellyfish Aequorea victoria, emits green light (emission peak at a wavelength of 509 nm) when excited byblue light (excitation peak at a wavelength of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell…

This set contains four CMV-driven vectors: SOX2 (PLV-10008), OCT3/4 (PLV-10007), KLF4 (PLV-10006) and C-MYC (PLV-10005).

LHX2 (LIM Homeobox 2) belongs to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. LHX2 is involved in neuronal differentiation. This lentivirus expresses human LHX2 under the control of the CMV promoter.

Microphthalmia-associated transcription factor (MITF) is a member of the basic/helix-loop-helix/leucine zipper (b-HLH-ZIP) transcription factor subfamily. MITF regulates the differentiation of melanocytes, optic cup-derived retinal pigment epithelium (RPE), and some types of bone marrow-derived…

Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

Luciferase is an enzyme that is catalyzes the oxidation of luciferin to generate bioluminescence. Firefly luciferase refers to the enzyme that was isolated from Photinus pyralis.

MYC (C-MYC) is a regulator gene that codes for a transcription factor is believed to regulate expression of 15% of all genes. C-MYC is one of the genes that has been used to generate iPS cells in culture. Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin…

NEUROD1 is a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and…

Calcium Phosphate (CaPO) Transfection Kit is perfect to transfect HEK 293 cells. This transfection method, first described by Graham and Van Der Ebb in 1973, has been optimized in order to reach higher transfection efficiency. The CaPO transfection kit is simple and easy to use. It allows reaching…

ADAM-17 gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. ADAM-17 is an…

Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the…

It has been observed that microRNA miR-182 plays a role in tumorigenesis and cancer metastasis. This lentivirus expresses miR-182 under the control of the Tet promoter.

RAR-related orphan receptor B (RORB) is a member of the NR1 subfamily of nuclear hormone receptors. Rorb has been shown to be involved in the differentiation of rod photoreceptor cells, Amacrine and horizontal interneurons and associated with bipolar disorder. This lentivirus expresses human RORB…

In vivo PolyMag™ Transfection Reagent has been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA, oligonucleotides using magnetic nanoparticles coated with specific polymers. DNA complexes can be easily administrated through various injection routes…