Nile Red is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments. It is a fluorescent stain for intracellular lipid droplets. Nile red can be used for the rapid determination of lipids by thin-layer chromatography or for staining whole…

Propidium iodide reagent is used for the fluorescent staining of nucleic acids. Propidium iodide (PI) binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4-5 base pairs of DNA. PI also binds to RNA, necessitating treatment…

Lucifer Yellow CH is the hydrazine (-NH-CO-NH-NH2) form of the dye compared to Lucifer Yellow VS, which is the vinyl sulfone (-Ph-SO2-CH=CH2) form. It is a highly fluorescent dye useful in marking nerve cells. Lucifer yellow CH has been used to demonstrate electrical coupling between hamster…