LGC, Biosearch Technologies Chemicals Reagents P
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Endura ElectroCompetent Cells
LGC, Biosearch TechnologiesLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…
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Episcript Rnase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity
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MasterPure Complete DNA & RNA Purification Kits
LGC, Biosearch TechnologiesThe MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.
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QuickExtract RNA Extraction Kit 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.
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QuickExtract Plant DNA Extraction Solution 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes.
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Terminator 5'-Phosphate-Dependent Exonuclease 40 U
LGC, Biosearch TechnologiesTerminator™ 5´-Phosphate-Dependent Exonuclease is a processive 5´′→3´ exonuclease that digests RNA that has a 5´-monophosphate end. It does not digest RNA that has a 5´-triphosphate, 5´-cap or 5´-hydroxyl group.
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E. cloni 10GF’ Electrocompetent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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NxGen T4 DNA Ligase
LGC, Biosearch TechnologiesT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
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Ampligase 10X Reaction Buffer 5 ml
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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QuickExtract FFPE DNA Extraction Kit 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ FFPE DNA Extraction Kit can be used for the isolation of DNA from FFPE samples for PCR-based analysis, e.g., microsatellite detection, SNP detection, tumor heterogeneity studies, copy number detection, methylation analysis, and Short Tandem Repeat (STR) analysis.
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Ampligase DNA Ligase
LGC, Biosearch TechnologiesAmpligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures
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TE Buffer 70 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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E. cloni 10G CLASSIC Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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E. cloni 10G Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of…
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Sbeadex viral RNA Purification Kit
LGC, Biosearch Technologiessbeadex viral RNA purification kits are magnetic-bead based kits are validated for research use only using AccuPlex™ reference material (SeraCare), replicating a real-life clinical sample as far as practically possible, and are compatible with Biosearch Technologies’ COVID-19 detection kit.…
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OverExpress Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s OverExpress Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins…
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Proteinase K 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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RNase A 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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RapiDxFire qPCR 5X Master Mix GF
LGC, Biosearch TechnologiesRapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…
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Endura Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be…
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RapiDxFire Hot Start Taq DNA Polymerase
LGC, Biosearch TechnologiesRapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst…
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TRANSFORMAX EC100 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.
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E. cloni EXPRESS BL21(DE3) Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…
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MasterAmp 10X PCR Enhancer 10 ml
LGC, Biosearch TechnologiesFor use with the MasterAmp™ PCR Optimization Kits.
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E. cloni 10G SUPREME Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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T7 R&DNA Polymerase 5,000 U
LGC, Biosearch TechnologiesT7 R&DNA™ Polymerase is a recombinant mutant form of T7 RNA polymerase (Y639F mutant), in which Tyr-639 has been replaced by Phe.
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E. cloni 10G ELITE Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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Exonuclease VII 250 U @ 10 U/µl
LGC, Biosearch TechnologiesExonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.
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SS320 (MC1061 F) Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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HI-Control 10G Chemically Competent Cells 12 rxns (SOLOs)
LGC, Biosearch TechnologiesThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.
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ER2738 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. ER2738 Electrocompetent Cells are an amber suppressor strain (glnV) prepared as highly efficient (=2 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New…