RapiDxFire™ Hot Start Taq DNA Polymerase is recombinant Taq DNA Polymerase from Thermus aquaticus bound to select blockers, which prevents unwanted, nonspecific amplification during reaction set up at temperatures less than 60 °C. Enzyme activity is restored after a short 15 second burst at 94 °C, enabling fast detection of low abundance target DNA. The Taq polymerase possesses 5'′→3’ polymerase activity (amplicons up to 5 Kb) and dsDNA specific 5′→3’ exonuclease activity, ideal for use with hydrolysis probes in qPCR reactions. The glycerol-free (GF), Triton®-free, and high- concentration formulation- is compatible with downstream lyophilisation methods and provides robust enzyme stability in liquid form (>28 days at 37 °C). These features provide flexible preparation and storage methods for qPCR master mixes (and detection assays) in addition to enabling benchtop reaction setups in warmer environments. Whether you are developing automated diagnostic tests or producing amplicons for downstream applications, RapiDxFire Hot Start Taq DNA Polymerase is your perfect choice for both demanding and routine PCR applications.