Reagents, P, Reagents, LGC, Biosearch Technologies
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QuickExtract DNA Extraction Solution
LGC, Biosearch TechnologiesThe QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
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Episcript Rnase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity
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CircLigase ssDNA Ligase
LGC, Biosearch TechnologiesCircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group
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Exonuclease III 25,000 U @ 200 U/µl
LGC, Biosearch TechnologiesExonuclease III digests duplex DNA in a 3´′→5´ direction from a nick, a blunt end, or 3´-recessed end, producing stretches of ssDNA on the opposite strand
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RIBONUCL-5'-TRIPHOSPHATE (NTP) SOL 25 umole ea
LGC, Biosearch TechnologiesOne tube each of ATP, CTP, GTP and UTP at 100 mM. Each is provided as a sterile, neutral solution.
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E. cloni 10G BAC-Optimized Electrocomp Cell 25 rxns
LGC, Biosearch TechnologiesE. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 10^7 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large…
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QuickExtract RNA Extraction Kit 50 ml
LGC, Biosearch TechnologiesThe QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.
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Ampliscribe T7 High Yield Transcription Kit 50 Rxns
LGC, Biosearch TechnologiesAmpliScribe™ T7 High Yield Transcription Kits are specially formulated to produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions.
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ClearColi BL21(DE3) Electrocompetent Cells
LGC, Biosearch TechnologiesClearColi® BL21(DE3) cells are the first commercially available competent cells with a modified LPS that does not trigger the endotoxic response in mammalian cells. ClearColi™ cells lack outer membrane agonists for hTLR4/MD-2 activation; therefore, activation of hTLR4/MD-2 signalling by…
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NxSeq Adaptors
LGC, Biosearch TechnologiesThe NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers. Each box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.
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Copycutter Epi400
LGC, Biosearch TechnologiesCopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins
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TG1 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…
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Riboguard Rnase Inhibitor
LGC, Biosearch TechnologiesRiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.
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Ampliscribe T7-Flash Transcription Kits
LGC, Biosearch TechnologiesThe AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.
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E. cloni 5-alpha Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…
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EpiScript RNase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity
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Red Cell Lysis Solution 1200 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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Ready-Lyse Lysozyme Solution
LGC, Biosearch TechnologiesReady-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.
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Durascribe T7 Transcription Kits
LGC, Biosearch TechnologiesThe DuraScribe® T7 Transcription Kit produces 2'-Fluorine-modified RNA transcripts—called DuraScript® RNA—that are completely resistant to RNase A. Ideal for SELEX RNA-aptamer selection procedures.
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NxGen phi29 DNA Polymerase
LGC, Biosearch Technologiesphi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
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RapiDxFire Thermostable Reverse Transcriptase
LGC, Biosearch TechnologiesRapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…
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E. cloni 10G CLASSIC Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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NxSeq AmpFREE Low DNA Library Kits
LGC, Biosearch TechnologiesThe NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours…
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LavaLAMP™ RNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which…
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RapiDxFire qPCR 5X Master Mix GF
LGC, Biosearch TechnologiesRapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X…
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NxGen M-MuLV Reverse Transcriptase
LGC, Biosearch TechnologiesM-MuLV Reverse Transcriptase is an RNA-dependent DNA polymerase which shows no measurable 3'′→5' proofreading function. This enzyme can copy a single-stranded DNA template or perform cDNA synthesis by extending a DNA primer annealed to an RNA template.
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E. cloni 10GF’ Electrocompetent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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EconoTaq DNA Polymerase
LGC, Biosearch TechnologiesEconoTaq’s low price is coupled with high quality and performance. QC specifications for EconoTaq are rigorous: Greater than 99% pure by SDS gel electrophoresis. No detectable DNA contamination as determined by PCR using generic primers. No detectable endonuclease (nicking)…
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LavaLAMP™ RNA Component Kits
LGC, Biosearch TechnologiesThe LavaLAMP™ RNA Component Kit is a deconstruction of the LavaLAMP™ RNA Master Mix, and as such, when reactions are setup using the initial Experimental reaction conditions outlined in the Component Kit User Manual, the composition of that reaction matches the composition of the…
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MC1061 F- Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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TRANSFORMAX EC100 CHEMICALLY COMP E. COLI 10 rxns
LGC, Biosearch TechnologiesChemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.