RapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90°C) and maintains its core activity after >3 months storage at 20-25°C. This enzyme lacks RNAse H and 3’ 5’ exonuclease activity and efficiently synthesizes short cDNA fragments (= 1 Kb). Like other reverse transcriptases, RapiDxFire RT possesses DNA polymerase activity but lacks 5' 3' exonuclease activity. Supplied in a glycerol-free, Triton™ X-100-free storage buffer, the enzyme formulation can be further optimized for downstream lyophilization.