EconoTaq® PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR. More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify…

The NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

This reagent is designed for use with the MasterPure™ kit protocols only.

TransforMax™ EC100™ Electrocompetent E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency.

Perfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…

Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´′→3´ and 3´′→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).

Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3´′→5´ exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures.

The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.

sbeadex viral RNA purification kits are magnetic-bead based kits are validated for research use only using AccuPlex™ reference material (SeraCare), replicating a real-life clinical sample as far as practically possible, and are compatible with Biosearch Technologies’ COVID-19 detection kit.…

CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins

The MasterPure™ Yeast RNA Purification Kit provides all of the reagents needed to purify RNA from yeast cell types (including Candida, Saccharomyces, Schizosaccharomyces) and filamentous fungi.

This reagent is designed for use with the MasterPure™ kit protocols only.

CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group

EconoTaq’s low price is coupled with high quality and performance. QC specifications for EconoTaq are rigorous: Greater than 99% pure by SDS gel electrophoresis. No detectable DNA contamination as determined by PCR using generic primers. No detectable endonuclease (nicking)…

Perfect for antibody phage display or peptide phage display library creation. ER2738 Electrocompetent Cells are an amber suppressor strain (glnV) prepared as highly efficient (=2 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New…

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…

The NxSeq® AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. We optimized each step of the protocol to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours…

Ampligase® Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures

The DuraScribe® T7 Transcription Kit produces 2'-Fluorine-modified RNA transcripts—called DuraScript® RNA—that are completely resistant to RNase A. Ideal for SELEX RNA-aptamer selection procedures.

The MasterPure™ Gram-Positive DNA Purification Kit provides all of the reagents needed to purify DNA from Gram-positive bacteria.

RapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…

Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis.

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time….We promise!

E. cloni 10G BAC-Optimized Electrocompetent Cells (> 1 × 10^7 cfu/µg 150 kb BAC DNA; > 1 × 1010 cfu/µg pUC DNA). The first competent cells developed by Lucigen specifically for large DNA cloning. Manufactured using a novel procedure that maximizes transformation efficiencies with very large…

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.

Ready-Lyse™ Lysozyme Solution is a recombinant lysozyme preparation, derived from a nonmammalian, nonavian source, that is useful for the lysis of Gram-negative (such as E. coli) and Gram-positive (such as Bacillus sp.) bacteria.

Electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.

The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.