Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal serum. The resulting whole antiserum is heat treated to inactivate complement and is adsorbed with normal pooled canine erythrocytes to remove nonspecific agglutinins. Canine…

The total protein is determined using the Biuret procedure, with bovine albumin as the standard. The product is tested for purity at a minimum of 20 mg/mL using immunoelectrophoresis. The IgG concentration is determined by radial immunodiffusion and is reported in mg/mL, and percentage of total…