It is rich in vitamins especially those belonging to B complex and is often used to supply these factors in culture media at a concentration of 0.3% to 0.5%. It is particularly used in media for cultivation of microorganisms encountered in milk or other dairy products. Yeast Extract Powder is…

This medium is prepared by using HiVeg hydrolysate which is free from BSE/TSE risks associated with animal based peptone. Terrific HiVeg Broth is the modification of Terrific Broth which is a very nutritious medium designed by Tartoff and Hobbs (1) to improve the yield of plasmid in Escherichia…

HiCrome Candida Differential Agar is recommended for rapid isolation and identification of cultures.

Beef Extract Paste is prepared from specially selected fresh lean beef for use in bacteriological culture media. It is highly nutritious and supports good growth of a wide variety of microorganisms. Owing to its nutritive value, it serves as a crude source of carbon and nitrogen, therefore…

Beef Extract powder Type I is prepared from lean beef, for use in bacteriological preparation and general purpose media. Appearance Light yellow to yellow Homogenous Free flowing powder,having Characteristic odor but not putrescent. Solubility Freely soluble in distilled/purified water,…

It is a purified component of Ox bile(free from bile acid) is recommended as a selective agent in microbiological culture media. Appearance Light yellow to light brown,Homogeneous Free flowing, hygroscopic powder having characteristic sharp odor, but not putrescent. Solubility Freely…

The coliform group consists of several genera of bacteria belonging to the family Enterobacteriaceae . The historical definition of this group has been based on the method used for detection i.e. lactose fermentation. This group is defined as all aerobic and facultative anaerobic, gram-negative,…

Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of…

Beef extract, certified is prepared from specially selected fresh, lean beef for use in bacteriological culture media. It is a mixture of peptides and amino acids, nucleotide fractions, organic acids, minerals and some vitamins, providing an undefined source of nutrients which supports good growth…

It is rich in carbohydrates and is ideally suitable for use in media employed for cultivation of fungi such as, Malt Agar, Malt Extract Agar, Yeast Malt Agar, etc. Malt Extract Powder is prepared from aqueous extract of sprouted malt grains and dried at low temperature to preserve nutrients…

Glucose Yeast Extract Agar is prepared according to the formula described by Evans and Niven (1) and Rogosa et al (2) and is used for enumeration and cultivation of Lactobacilli in pharmaceutical preparations. The medium contains a variety of salts like sulphates, phosphates to support the growth…

Lactic Acid Bacteria Selective Agar Base is based on the formula of Saha, Sondag and Middlekauff for the detection of lactic acid bacteria in beer and brewing processes (1). It is recommended by European Brewing convention (EBC) and the American Society of Brewing Chemists for isolation of…

Folic Acid Assay Medium is recommended for microbiological assay of Folic Acid using Enterococcus hirae ATCC 8043 (formerly Streptococcus faecalis ATCC 8043) as the test organism. Folic Acid Assay Medium contains all the necessary nutrients for the growth of the test organism except folic acid. The…

Broth used for sterility testing of turbid or viscous biological products.

Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1, 2). Nutrient Agar is ideal for demonstration and teaching purposes…

Peptone Water is particularly suitable as a substrate in the study of indole production. Peptic digest of animal tissue used in Peptone Water is rich in tryptophan content. Presence of indole can be demonstrated using either Kovacs or Ehlrich reagent. Peptone Water is also utilized as a base for…

Nutrient Broth Medium is a general purpose medium used for the examination of water and dairy products according to Standard Methods for the Examination of Water and Wastewater (1) and Dairy Products (2) in accordance with IP (4). It can also be used for cultivating several less fastidious…

The agents of brucellosis, Brucella species are normal flora of the genital and urinary tracts of many animals including goats, pigs, cows and dogs. Most humans acquire the disease through ingestion of contaminating milk or through occupational exposure; the disease is particularly common among…

An antibiotic supplement recommended for the selective isolation of Brucella species from milk. Storage and Shelf Life Store at 2 - 8°C. Use before expiry date on the label.

The membrane filter technique is extremely useful in monitoring water samples. As related to the membrane filter technique, the coliform group is defined as those facultative anaerobic, gram-negative, non-spore-forming, rod-shaped bacteria that develop red colonies with a metallic sheen within 24…

Triple Sugar Iron Agar was originally proposed by Sulkin and Willett (1) and modified by Hajna (2) for identifying Enterobacteriaceae . This medium complies with the recommendation of APHA, for the examination of meat and food products (3), for the examination of milk and dairy products (4) and…

Brucella are intracellular parasites that cause epizootic abortions in animals and septicemic febrile illness or localized infections of bone, tissue or organ systems in humans (1, 2). Brucella species are highly fastidious and therefore require a nutrient rich medium to be able to grow. Also, …

Plate Count Agar is formulated as described by Buchbinder et al (1) which is recommended by APHA (2,3,4) and FDA (5). Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends the use of pour plate…

Acetamide Agar is formulated as per the recommendation of Standard Methods for the Examination of Water and Wastewater (1). Gilardi and others showed that a wide variety of non-fermenting organisms were capable of utilizing acetamide by using basal mineral media (2, 3). However very few organisms…