HEPES 1M Solution. Used in cell culture media to maintain the pH levels of basal media.

Pseudomonas aeruginosa grows well on all normal laboratory media but specific isolation of the organism, from environmental sites or from human, animal or plant sources, is best carried out on a medium, which contains a selective agent and also constituents to enhance pigment production. Most…

The heterotrophic plate count (HPC), formerly known as the standard plate count is a procedure for estimating the numbers of live heterotrophic bacteria in water and measuring the changes during water treatment and distribution or in swimming pools. Different methods namely pour plate method,…

Microorganisms in an aquatic environment may occur at all depths ranging from the surface region to the very bottom of the ocean trenches. The top layers and the bottom sediments harbour higher concentration of microorganisms (1). Marine microorganisms are vital to ecological cycles because they…

Minimum Essential Medium (MEM) is a modification of Basal Medium Eagle (BME). It was developed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of Hela cells and normal mammalian fibroblasts. MEM includes higher concentration of amino acids so as to closely…

Tryptone Glucose Yeast Extract Agar was originally developed by Bowers and Hucker (1) which they called as Tryptone Glucose Skim Milk Agar. Later on it was modified to the present composition for the cultivation and enumeration of bacteria in air, water (2), milk and dairy products (3). Various…

Candida are yeast-like fungus forming normal flora inhabiting the mouth and throat, the intestinal tract and the genital tract. Under certain conditions, they cause life-threatening diseases particularly in immunocompromised patients. Candida albicans is the species most commonly isolated…

Being rich in essential amino acids it is used to supplement Tissue Culture Media and fermentation media. It is rich in tryptophan content, therefore, used in Indole test. It is included in preparation of baculovirus serum free medium for production of recombinant protein. It is also used in…

HiCrome E.coli Agar is recommended for the detection and enumeration of confirmation on membrane filter or by indole reagent.

Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham (DMEM/F12, 1:1 mixture) was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells. …

Triple Sugar Iron Agar was originally proposed by Sulkin and Willett (1) and modified by Hajna (2) for identifying Enterobacteriaceae . This medium complies with the recommendation of APHA, for the examination of meat and food products (3), for the examination of milk and dairy products (4) and…

Enterococci are indicators of the sanitary quality of recreational waters, since they occur in faeces of humans and warmblooded animals (1). Detection and quantitation of Enterococci is necessary because gastroenteritis is associated with swimming in recreational water, which is dependant of…

Luria Agar is prepared as described by Lennox (1) for cultivation and maintenance of recombinant strains of Escherichia coli . The media is generally used for molecular and genetic studies, because of its nutritive capacity and simple composition, which can be easily altered as per specific…

Agar used for the cultivation of yeasts, moulds and aciduric microorganisms.

A combination of 12 tests for differentiation of Enterobacteriaceae species. (Kit contains sterile media for Indole, Methyl red, Voges Proskauer?s, Citrate utilization tests and 8 different carbohydrates- Glucose, Adonitol, Arabinose, Lactose, Sorbi.