It is rich in vitamins especially those belonging to B complex and is often used to supply these factors in culture media at a concentration of 0.3% to 0.5%. It is particularly used in media for cultivation of microorganisms encountered in milk or other dairy products. Yeast Extract Powder is…

Beef Extract Paste is prepared from specially selected fresh lean beef for use in bacteriological culture media. It is highly nutritious and supports good growth of a wide variety of microorganisms. Owing to its nutritive value, it serves as a crude source of carbon and nitrogen, therefore…

HiCrome Vibrio Agar is recommended for the isolation, and selective chromogenic differentiation of seafood.

Nutrient Broth Medium is a general purpose medium used for the examination of water and dairy products according to Standard Methods for the Examination of Water and Wastewater (1) and Dairy Products (2) in accordance with IP (4). It can also be used for cultivating several less fastidious…

Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of…

SS Agar medium is recommended as differential and selective medium for the isolation of Salmonella and Shigella species from pathological specimens (1) and suspected foodstuffs (2, 3, 4, 5) and for microbial limit test (6). SS Agar is a moderately selective medium in which gram-positive…

Glucose Yeast Extract Agar is prepared according to the formula described by Evans and Niven (1) and Rogosa et al (2) and is used for enumeration and cultivation of Lactobacilli in pharmaceutical preparations. The medium contains a variety of salts like sulphates, phosphates to support the growth…

Microorganisms in an aquatic environment may occur at all depths ranging from the surface region to the very bottom of the ocean trenches. The top layers and the bottom sediments harbour higher concentration of microorganisms (1). Marine microorganisms are vital to ecological cycles because they…

Microorganisms in an aquatic environment may occur at all depths ranging from the surface region to the very bottom of the ocean trenches. The top layers and the bottom sediments harbour higher concentration of microorganisms (1). Marine microorganisms are vital to ecological cycles because they…

Recommended for detection of urease activity.

Clostridium perfringens food poisoning is one of the most common types of human food borne illness (1). The foods usually involved are cooked meat or poultry products containing large numbers of viable cells. A heat- labile enterotoxin produced only by sporulating cells (2) induces the major…

MacConkey Broth Purple w/ BCP is a modification of MacConkey Medium (1). Childs and Allen (2) demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. MacConkey Broth…

Recommended for isolation and cultivation of Alicyclobacillus in fruit juices in accordance with Official Method of IFU.

Casein Peptone is enzymic digest of casein. It is manufactured under controlled conditions. Casein being a rich source of amino nitrogen, the hydrolysed product casein peptone obtained also contains peptides, aminoacids etc. which acts as nitrogen source for microorganisms when used in media…

A combination of 25 tests for identification of Enterobacteriaceae species. (Kit contains discs for oxidase test and sterile media for ONPG, Lysine utilization, Ornithine utilization, Urease detection, Phenylalanine deamination (TDA), Nitrate reduct.

Plate Count Agar is equivalent to the medium recommended by APHA for the isolation of microorganisms in milk and other dairy products (1). Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends pour…

Examination of water, foods, ingredients and raw materials, for the presence of marker groups such as coliforms is one of the most common tests in a microbiology laboratory, partly because of the relative ease and speed with which these tests can be accomplished. Where it is claimed that drinking…

Methyl Red and Voges-Proskauer test are among the two various tests used in the biochemical identification of bacterial species. These tests were originally studied by Voges, Proskauer (1) and subsequently by Clark and Lubs (2) to differentiate between members of the coli- aerogens group. Both the…

Tryptone, Certified is manufactured by a controlled enzymatic hydrolysis of Casein. It is rich in tryptophan and used by a wide variety of microorganisms, recommended in a number of media such as Sterility Testing media, Diagnostics Media, media for biochemical characterization etc. Appearance …

Eosin Methylene Blue (EMB) Agar was originally devised by Holt-Harris and Teague (1) and further modified by Levine (2). The above medium is a combination of the Levine and Holt-Harris and Teague formulae which contains peptic digest of animal tissue and phosphate as recommended by Levine and two…

Burman (1) substituted bile salts with teepol in Membrane Enriched Teepol Broth, the membrane filtration test medium used to detect coliform organisms in water. Membrane or M-Lauryl Sulphate Broth recommended (2, 3) is similar to M-Enriched Teepol Broth except teepol has been replaced by sodium…

Cetrimide Agar was described by King et al (1). This media is based on the formulation described in BP(3) and is in accordance with the harmonized method of USP/EP/IP/JP (2,4,5,7). It is used as a selective medium for the isolation of Pseudomonas aeruginosa from pharmaceutical products. This…

Dubos and Middlebrook (1) developed various formulations containing oleic acid and albumin, which protect Mycobacterium from toxic agents, helping for the growth of tubercle bacilli. Middlebrook 7H10 Agar Base was formulated as per Middlebrook, Cohn et al (2) reformed the original oleic…

Fungi were among the first microorganisms recognized because some of the fruiting structures, such as the mushrooms, are large enough to be seen without a microscope. Fungi can be grouped simply on the basis of morphology as either yeasts or moulds (1). Fungal diseases that occur on the skin, hair…