The most common disease caused by Corynebacterium diphtheriae is diphtheria, an acute communicable disease manifested by both local infection of the upper respiratory tract and the systemic effects of the toxin, which are most notable in the heart and peripheral nerves (1). Hoyle Medium Base,…

Granulated Casein Soyabean Digest Agar (Soyabean Casein Digest Agar) (Tryptone Soya Agar) is a general purpose medium used for cultivation of a wide variety of microorganisms and for Sterility testing in pharmaceutical procedures.

Glucose Yeast Extract Agar is prepared according to the formula described by Evans and Niven (1) and Rogosa et al (2) and is used for enumeration and cultivation of Lactobacilli in pharmaceutical preparations. The medium contains a variety of salts like sulphates, phosphates to support the growth…

Granulated BLE Broth Base (Buffered Listeria Enrichment Broth Base) is recommended by FDA Committe for enrichment procedure for isolation of Listeria monocytogenes.

Klett (1) first demonstrated the selective inhibitory effects of selenite and Guth (2) used it to isolate Salmonella serotype Typhi. Leifson fully investigated selenite and formulated the media. Selenite Cystine Medium is a modification of Leifsons (3) formula with added cystine (4). Modification…

It can be used successfully in following culture media in place of Beef Extract: General purpose media : Beef Extract Agar, HiVeg (MV806) / HiVeg Broth (MV807) and Nutrient HiVeg Agar(MV001) / Broth ( MV002) etc. Diagnostic media : CLED HiVeg Agar (MV352/MV792), DCLS HiVeg Agar (MV160), TSI…

Plate Count Agar originally formulated by Buchbinder et al (1) which is recommended by APHA (2,3,4) FDA (5) and ISO committee (6). Present formulation is recommended by BIS (7) for enumeration of microorganisms in food, water and wastewater. Casein enzymic hydrolysate provides amino acids and other…

A combination of 12 tests for identification of Candida species. (Kit contains sterile media for Urease production and 11 different carbohydrates utilization tests.

Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of…

Granulated Potato Dextrose Broth is for cultivation and enumeration of yeasts and moulds.

HiEncap™ MacConkey Agar w/o CV, NaCl w/ 0.5% Bile Salts is recommended for the cultivation and differentiation of enteric bacteria, restricting swarming of Proteus species from specimen samples. Each capsule contains 11.77 gms of medium. Suspend 1 capsule in 250 ml (4 capsules in 1000 ml) distilled…

An antibiotic supplement recommended for selective isolation of Legionella species. Storage and Shelf Life Store at 2-8°C. Use before the expiry date on the label.

MacConkey Agar is the earliest selective and differential medium for cultivation of enteric microorganisms from a variety of clinical specimens (1, 2). The original medium contains protein, bile salts, sodium chloride and two dyes. MacConkey Agar w/ Bromo thymol blue is a modification of the…

Medium base for selective isolation and enumeration of yeasts and moulds associated with food spoilage.

Plate Count Agar is equivalent to the medium recommended by APHA for the isolation of microorganisms in milk and other dairy products (1). Casein enzymic hydrolysate provides amino acids and other complex nitrogenous substances. Yeast extract supplies Vitamin B complex. APHA recommends pour…

Casein Acid Hydrolysate is used in preparation of microbiological media. It is an acid hydrolysate of casein. It has Sodium chloride less than 3% and is Vitamin free. Appearance Off white to light yellow Homogenous Free flowing powder, having Characteristic odor but not putrescent. …

Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1, 2). Nutrient Agar is ideal for demonstration and teaching purposes…

Microorganisms in an aquatic environment may occur at all depths ranging from the surface region to the very bottom of the ocean trenches. The top layers and the bottom sediments harbour higher concentration of microorganisms (1). Marine microorganisms are vital to ecological cycles because they…

Brain Heart Infusion Medium is useful for cultivating a wide variety of microorganisms since it is a highly nutritive medium. It is also used to prepare the inocula for antimicrobial susceptibility testing. Brain Heart Infusion Broth is a modification of the original formulation of Rosenow, where…