Potato Dextrose Agar is recommended by APHA (1) and F.D.A. (2) for plate counts of yeasts and moulds in the examination of foods and dairy products (3). Potato Dextrose Agar is also used for stimulating sporulation, for maintaining stock cultures of certain dermatophytes and for differentiation of…

It is a purified component of Ox bile(free from bile acid) is recommended as a selective agent in microbiological culture media. Appearance Light yellow to light brown,Homogeneous Free flowing, hygroscopic powder having characteristic sharp odor, but not putrescent. Solubility Freely…

Glucose Yeast Extract Agar is prepared according to the formula described by Evans and Niven (1) and Rogosa et al (2) and is used for enumeration and cultivation of Lactobacilli in pharmaceutical preparations. The medium contains a variety of salts like sulphates, phosphates to support the growth…

Coliforms are considered to be members of Enterobacteriaceae , which grow in the presence of bile salts and produce acid and gas from lactose within 48 hours at 37°C (1). These bacteria can also be defined as, members of Enterobacteriaceae capable of growing at 37°C, that normally…

Casein Peptone is enzymic digest of casein. It is manufactured under controlled conditions. Casein being a rich source of amino nitrogen, the hydrolysed product casein peptone obtained also contains peptides, aminoacids etc. which acts as nitrogen source for microorganisms when used in media…

It is rich in vitamins especially those belonging to B complex and is often used to supply these factors in culture media at a concentration of 0.3% to 0.5%. It is particularly used in media for cultivation of microorganisms encountered in milk or other dairy products. Yeast Extract Powder is…

Microorganisms in an aquatic environment may occur at all depths ranging from the surface region to the very bottom of the ocean trenches. The top layers and the bottom sediments harbour higher concentration of microorganisms (1). Marine microorganisms are vital to ecological cycles because they…

Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (1, 2). Nutrient Agar is ideal for demonstration and teaching purposes…

It is used in culture media used for bulk production of antibiotics, enzymes, veterinary preparations and bacterial toxins. It is used in synthetic media in acclimatization of microorganisms in bioreactor studies. Also, it finds application as lyoprotectant in preparation of freeze-dried cultures…

Casein Acid Hydrolysate is used in preparation of microbiological media. It is an acid hydrolysate of casein. It has Sodium chloride less than 3% and is Vitamin free. Appearance Off white to light yellow Homogenous Free flowing powder, having Characteristic odor but not putrescent. …

Tributyrin HiVeg Agar Base is specially developed from HiVeg peptone to avoid BSE/ TSE risks associated with animal origin Peptic digest of animal tissue. This medium is the modification of Tributyrin Agar Base, which was originally formulated by Anderson (1) for the detection and enumeration of…

It is used to prepare culture media for cultivation of Neisseria, Yersinia, Staphylococci & Streptococci. It can also be used for cultivation of other fastidious bacteria on a large scale. Peptone Special is an enzymatic digest of animal tissues specifically designed to maximize the growth of…

Peptonized Milk is an enzymic digest of high grade skimmed milk powder. It has high tryptophan content and is therefore used in media for testing the indole reaction. It serves as a source of nitrogen and also has high level of carbohydrate. Suitable for cultivation of Lactobacilli, yeasts and…

Brain Heart Infusion Powder is a mixture of calf brain infusion and beef heart infusion. Each 17.5 grams of the powder contains infusions from 200 grams calf brain and 250 grams beef heart. It is a yellowish brown coloured, free flowing powder having characteristic but not putrescent odour. It…

Examination of water, foods, ingredients and raw materials, for the presence of marker groups such as coliforms is one of the most common tests in a microbiology laboratory, partly because of the relative ease and speed with which these tests can be accomplished. Where it is claimed that drinking…

It is rich in carbohydrates and is ideally suitable for use in media employed for cultivation of fungi such as, Malt Agar, Malt Extract Agar, Yeast Malt Agar, etc. Malt Extract Powder is prepared from aqueous extract of sprouted malt grains and dried at low temperature to preserve nutrients…

The most common disease caused by Corynebacterium diphtheriae is diphtheria, an acute communicable disease manifested by both local infection of the upper respiratory tract and the systemic effects of the toxin, which are most notable in the heart and peripheral nerves (1). Hoyle Medium Base,…

The Mueller Hinton formulation was originally developed as a simple, transparent agar medium for the cultivation of pathogenic Neisseria species (2). Other media were subsequently developed that replaced the use of Mueller Hinton Agar for the cultivation of pathogenic Neisseria species, but it…

Soyabean Casein Digest Medium is recommended by various pharmacopeias as a sterility testing and as a microbial limit testing medium (1, 2, 3). This medium is a highly nutritious medium used for cultivation of a wide variety of organisms (4). The combination of pancreatic digest of casein and…

Sabouraud Chloramphenicol Agar is cited as Medium C and recommended for cultivation of yeasts and moulds. This medium was described originally by Sabouraud (1) for the cultivation of fungi, particularly useful for the fungi associated with skin infections. The medium is often used with antibiotics…

Beef Extract Paste is prepared from specially selected fresh lean beef for use in bacteriological culture media. It is highly nutritious and supports good growth of a wide variety of microorganisms. Owing to its nutritive value, it serves as a crude source of carbon and nitrogen, therefore…