Tissue Culture Media
The primary purpose of tissue culture media is to provide a synthetic environment within the lab. This synthetic environment then allows cells to thrive and carry out normal functions.
Ideal for primary cell isolation and cell line maintenance, culture media is essential for the isolation and propagation of primary cells obtained from tissues.
PBS cell culture media is recommended for use when investigating the localization and distribution of target proteins in different parts of biological tissue.
Tissue culture media accessories include reagent-grade water, antibiotics, and Corning BioCoat cell culture inserts to assist you with achieving clear and accurate results.
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Hanks' Balanced Salt Solution (HBSS) without Phenol Red
Quality BiologicalHanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport…
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McCoy's 5A Medium
HiMediaMcCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to…
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Phosphate Buffered Saline, pH 7.2 10X. Commonly used for tissue disaggregation and monolayer dispersal.
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Application Phosphate buffered saline has been used as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from endometrium or menstrual blood. [1] General description Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is…
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Corning® BioCoat™ Gelatin Flasks
CorningCorning BioCoat Gelatin cultureware provides an attachment and growth promoting substrate for the culture of a variety of cell types. Gelatin is commonly used in the culture of vascular endothelial cells, muscle, embryonic stem (ES) cells, and F9 teratocarcinoma cells. It is also suitable for…
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Iscove's DMEM
bioWORLDGuilbert and Iscove published their modifications to Dulbecco's Modified Eagle's Medium (DMEM) in 1976. Their modification included the supplements of selenium, additional vitamins and amino acids, sodium pyruvate and HEPES buffer. In addition, ferric nitrate was replaced with potassium…
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The Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and…
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In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor…
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DMEM without Phenol Red & L-Glutamine
Quality BiologicalDMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. With High Glucose Without L-Glutamine Without Phenol red Without HEPES Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,…
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The HiMedia water/sterile/tissue culture grade endotoxin tested is mainly used in research facilities and chemical laboratories to conduct wide range of experiments. This DEPC treated compound is nuclease and protease free. It is available in sturdy bottle that endures heavy and rigorous usage.
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Iscove's Modified Dulbecco's Medium (IMDM)
Quality BiologicalThis modification of Dulbecco's MEM was developed by Dr. Iscove and his coworkers to assist in their studies of hematopoietic stem cells. They modified Dulbecco's MEM by the addition of HEPES buffer, selenium, additional amino acids, vitamins and sodium pyruvate, and potassium nitrate…
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Hanks' Balanced Salt Solution 10X w/ Phenol red w/o Sodium bicarbonate. Used with cells maintained in less CO2 environment or CO2 free environment.
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Poloxamer 188
CorningSpecialized for use in insect cell culture Poloxamer 188 is a surfactant used in cell culture to protect cells in suspension against possible damage during transfer, freezing and thawing, and stirring. It is a non-ionic detergent with an average molecular weight of 8400Da consisting of a central…
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For some applications, the use of a combination of ECM proteins, such as Laminin (LM) and Fibronectin (HFN) or LM and attachment factors such as Poly-D-Lysine (PDL) or Poly-L-Orinthine (PLO) has been shown superior to the use of either alone. Corning BioCoat PDL/LM and PLO/LM Cultureware is…
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Corning® Growth Factor
CorningThe Corning portfolio of cytokines, growth factors, and media supplementation offers a wide array of proteins that result in proliferation or differentiation of the affected cells. Vialed, highly purified growth factors with high biological activity Broad range of highly purified natural and…
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Nutrient Mixture F-12 Ham, Coon’s Modification w/Sodium bicarbonate w/o L-Glutamine.
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McCoy's 5A medium was originally formulated to meet the culture requirements of Novikoff Hepatoma cells, and has been found to support the indefinite proliferation of Walker 256 carcinoma cells along with a wide range of human and rat normal and transformed cells.
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Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells through its central-binding domain RGD sequence. HFN is a product of most mesenchymal and epithelial cells and is present in both the ECM and plasma. The principal function of HFN…
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Poly-D-Lysine (PDL) and Poly-L-Lysine (PLL) are synthetic compounds that enhance cell adhesion and protein absorption by altering surface charges on the culture substrate. In addition to promoting cell adhesion, poly-lysine surface treatments support neurite outgrowth and improve the survival of…
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Interleukin-2 (IL-2), mouse recombinant, plays a central role in cell-mediated immune response. Was first identified by its ability to promote the long-term proliferation of activated T-cells. (25,000 BRMP units) References: 1. Hatakeyama, M. and Taniguchi, T., "Interleukin-2" in Peptide Growth…
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QBSF®-60 Serum Free Medium
Quality BiologicalQBSF-60 is a proprietary serum-free medium that supports the expansion of human hematopoietic stem/progenitor cells. QBSF-60 supports CD34+ cells derived from normal bone marrow, cord blood, peripheral blood stem cells mobilized with cytokines for ex-vivo expansion and differentiation to…
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Technical Advantage: Product manufactured under industry-leading standards for low endotoxin content and superior results Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain…
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Ham's F-12 Nutrient Mixture without Hypoxanthine
Quality BiologicalCompared to other basal media, Ham’s F-12 contains a wider variety of components, and no proteins or growth factors. Without hypoxanthine and L-glutamine Ham’s F-12 uses a sodium bicarbonate buffer system (1.176 g/L); therefore requiring a 5–10% CO2 environment to maintain physiological pH. …
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Trace Elements A, B, and C
CorningTrace Elements A provides copper, zinc, iron, and selenium. Selenium aids in the detoxification of free radicals as a cofactor for GSH synthetase, while iron, copper, and zinc may be bound by serum protein. The combination of Trace Elements A contains the trace elements of MCDB 104 and of MCDB…
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Human Serum - Normal
bioWORLDHuman plasma is collected from healthy male donors at FDA-licensed facilities located entirely within the U.S. These individual units are converted to serum and then pooled, filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. Each individual unit is tested and found…
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The Corning® BioCoat® Poly-D-Lysine coverslip and dish is perfect for enhanced cell attachment and growth of a variety of primary cells as well as transformed cells in serum-free or serum-containing cultures..
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Thomas No. B003K43 A modified forumulation of Minimal Essential Medium, Improved Minimum Essential Medium (IMEM) contains a higher concentration of many components used in MEM along with additional amino acids, vitamins, and metabolites. Complete Certificate of Analysis available…
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The insect cell culture medium was formulated to closely resemble insect hemolymph, a compound crucial to the function of the insect circulatory system. The basal medium, when properly supplemented, has been used to culture cells derived from a variety of insects including several species of…
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EMEM without L-Glutamine
Quality BiologicalEagle’s minimal essential medium (EMEM) is a cell culture medium developed by Harry Eagle that can be used to maintain cells in tissue culture. Basal medium with different variations. Applications MEM was developed by Dr. Harry Eagle to overcome problems encountered when attempting to grow…