Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. It is most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatase is used…

β-Glucosidase is a glucosidase enzyme. It is an exocellulase with specificity for a variety of β-D-glycoside substrates. β-Glucosidase is used in the synthesis of glucosides and fucosides with various potential applications in pharmaceutical, cosmetic and detergent industries,…

Pectinase is a macerating enzyme comprised of pectintranseliminase, polygalacturonase, pectinesterase, xylanase, hemicellulases and cellulases. Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase causes random hydrolysis of…

HRP is a plant glycohemoprotein that belongs to the ferroprotoporphyrin group of peroxidases. HRP is composed of seven isozymes. All isozymes contain photohemin IX as prosthetic group. Neutral and amino sugars account for approximately 18% of the enzyme. The iron-containing hemin group is…

Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6 phosphate and D-fructose 6-phosphate. Responsible for the second step of glycolysis Involved in glucogenesis Highly conserved in bacteria and eukaryotes Phosphoglucose Isomerase fuctions as an…

Alcohol oxidase has been detected in several strains of yeast, such as Candida, Pichia, and Hansenula, that utilize methanol as a sole carbon and energy source. Some of these enzymes have been isolated and partially characterized. Alcohol oxidase plays a major role in the metabolism of methanol…

α-Thrombin is a trypsin-like serine protease involved in a multitude of processes in the human body. Thrombin generation is the result of limited proteolysis of the vitamin K-dependent zymogen prothrombin. Thrombin is the last enzyme in the clotting cascade functioning to cleave fibrinogen to…

Alcohol dehydrogenases are a group of dehydrogenase enzymes that occur in many organisms. It is a metalloenzyme containing four tightly bound zinc atoms per molecule. Yeast alcohol dehydrogenase (yADH) serves as an excellent model system for enzyme-catalyzed H transfer because unlike many other…

Pyruvate kinase from rabbit muscle is a tetramer consisting of four equal subunits of molecular weight 57 kDa. Pyruvate kinase catalyzes the following reaction: ADP + Phosphoenolpyruvate → Pyruvate + ATP . Both Mg2+ and K+ are required metal cofactors for optimal activity. The enzyme is…