Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation. The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second most…

G-Biosciences’ ML (Modified Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris(see tables 1 and 2).The…

A 1µl Assay For Rapid Estimation of DNA, RNA and Oligo Concentration NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. The system uses a unique combination of proprietary reagents and test strips and is an…

An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color…

Lumino™ ATP Detection Assay is designed for rapid, convenient and quantitative detection of adenosine-5’-triphosphate (ATP). It’s a bioluminescent assay which is based on requirement of ATP by luciferase enzyme for production of light. ATP Detection Assay has widespread…

Protn-Latex ™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minimum of skill or instrumentation. The test detects as low as 25-40µg protein /gm latex (or…

The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA…

The Bicinchoninic Acid (BCA) Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. The Bicinchoninic Acid (BCA) Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the…

The Fluorescent β-Galactosidase Assay (MUG) is a highly sensitive, fluorescent assay for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct The study of the lac operon has played an important role in…

The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an alternative to 51 Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple evaluation, characteristic of radioisotope release assays, with the…

TMRE Mitochondrial Membrane Potential Assay quantifies changes in mitochondrial membrane potential of live cells via microplate fluorometry, fluorescence microscopy and flow cytometry. Tetramethylrhodamine, ethyl ester (TMRE) is a cell permeable, positively charged red-orange dye that…

A highly sensitive, colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents, such as reducing agents, chelating agents,…

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers…

ATP serves as a marker for cell viability as it is present in all metabolically active cells.Lumino™ Cell Viability Glow Assay is a bioluminescent assay to detect and/or quantify ATP and thus measures the viable cells.The bioluminescent assay is based on requirement of ATP by luciferase…

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change color in response to the chemical reduction of growth medium due to cell…

β-Galactosidase Assay (CPRG) provides an easy, rapid, and highly sensitive method for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The β-Galactosidase Assay (CPRG) assay uses chlorophenol…

The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline…

A novel protein assay that is suitable for single sample or high throughput protein estimation. The SPN™ and SPN™- htp protein assays are rapid assays that are suitable for as little as 0.5µg protein and are resistant to interference from common laboratory agents. The assays are…

Firefly Luciferase Assays are one of the widely used reporter assays to detect and measure gene expression, function and regulation. G-Biosciences Lumino™ Firefly Luciferase Assay is designed for rapid and high sensitive detection or quantification of firefly luciferase reporter enzyme…

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP. The Bicinchoninic Acid…

CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells. Adherent or…

Lumino™ Firefly Luciferase Glow Assay is designed for high throughput screening or quantification of luciferase expression in mammalian cells which is mainly performed by batch processing of 96- and 384- well plates. Lumino™ Firefly Luciferase Glow Assay is homogenous,…

Calcein AM or Calcein acetoxymethyl ester is a hydrophobic compound which passes easily through cell membrane into live cells and is used for cell viability assays. The non-fluorescent calcein AM dye is hydrolyzed by cellular esterases to give calcein which is fluorescent and is retained in the…

NAD+/NADH Assay [Colorimetric] is designed for sensitive detection of intracellular nucleotides, NADH, NAD+ and their ratio. The kit specifically detects NAD+, NADH and not NADP. This colorimetric assay is based on NAD cycling enzyme mix which increases the sensitivity of detection. Features:…

Methylation of key biological molecules and proteins plays important roles in numerous biological systems, including signal transduction, biosynthesis, protein repair, gene silencing and chromatin regulation. The S-adenosylmethionine (SAM) dependent methyltransferases use SAM, the second…

Sensitive protease screening is needed to study protease activity present in the sample of interest. The Protease Screening Kit provides you with a simple and quick method for testing your samples for proteolysis. Simply incubate your sample in the reagent provided and obtain results. The…

PEROX say ™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe 2+ ) ions to ferric (Fe 3+ ) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH…

G-Biosciences’ CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing…

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the…

PhosphoQuant ™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrolysis of phosphates from seryl and threonyl residues in phosphoproteins and the subsequent…

JC-1 Mitochondrial Membrane Potential Assay comprise of lipophilic cationic fluorescent dye; 5,5,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimi-dazoylcarbocyanine iodide (JC-1). In healthy cells, JC-1 enters the energized mitochondria and form aggregates which change the fluorescent…

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community. ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening…