ATP serves as a marker for cell viability as it is present in all metabolically active cells.Lumino™ Cell Viability Glow Assay is a bioluminescent assay to detect and/or quantify ATP and thus measures the viable cells.The bioluminescent assay is based on requirement of ATP by luciferase enzyme for production of light . Viable cells are measured by quantifying ATP which in turn is measured by measuring luminescence.
The luminescent ATP based method for detection of viable cells is highly sensitive when compared to other cell viability assays that use colorimeter or fluorimeter. In addition the ATP based assay is rapid when compared to other commonly used methods for cell viability such as MTT, Alamar Blue or Calcein-AM as these assays require extended incubation steps to let the cellular metabolic machinery process the indicator molecules into a detectable signal
Features:
Homogenous assay: does not require removal of cell medium and cell debris. Simply add the Cell Viability Glow Reagent (formed after mixing substrate and buffer) and measure luminescence
Suitable for HTS assays: Stable signal: Glow type bioluminescent signal with signal half-life of greater than 3 hrs.
High sensitivity: Can detect as low as 10-12M (10-16 moles) ATP. Sensitivity also depends upon the type of luminometer and its settings.
Broad detection range and excellent linearity: Broad detection range of 10-12M to 10-4M ATP. Linear over high-log order (5 or more) of ATP concentration. The detection and linear range is also dependent on the sensitivity of the luminometer.
Available in 100, 500 and 1000 assays format with use of 96-well opaque microtiter plate.
Rapid, simple and user friendly
Application(s):
Lumino™ Cell Viability Glow Assay is designed measure viable eukaryotic cells
The assay can be used to measure cell proliferation, apoptosis or cytotoxicity.