EC Number 231-791-2 Other Notes 0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2 Substrates Substrate for visualizing alkaline phosphatase conjugates in Western blotting. Legal Information BCIP is a registered trademark of Sigma-Aldrich Co. LLC