Buffer Solutions

A buffer is a solution created to resist pH changes when introducing internal and external influences. Buffers also maintain intercellular and extracellular pH within the physiological range, providing minimal interference in biological processes. At Thomas Scientific, we have an extensive range of buffers available, including sodium phosphate buffers, blue buffers and LDS sample buffers. We offer a selection of buffers and native gels that carry both positive and negative charges. Buffers are suitable for biochemical research applications such as cell cultivation, isolation of cells, and enzyme assays.

Buffer Solutions

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  • Color-Coded pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General Traceable to NIST standards

  • Clear pH Buffer Solutions

    chemForce® (powered by Thomas Scientific)

    Harmonized #: 2806.10.0000|0000 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Colorless Physical State: Liquid Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • Color-Coded pH Buffers

    Standardized and certified traceable to NIST Standard Color-coded for quick reference Accuracy ±0.01 pH at 25°C pH temperature chart, lot numbers and expiration dates on each label pH 4.0 buffer is colored red, 7.0 buffer is yellow or green and 10.0 buffer is blue. Thomas numbers 4120R54,...

  • pH Electrode Solutions

    Thermo Scientific Orion

    Electrode Storage Solution 475 mL plastic bottle pH of solution is 6.2 to 7.4 For short term storage of all electrodes for one day to a week. Electrode Filling Solution Internal filling solution for Ross-type electrodes (3M KCl). In packages of five 60 mL bottles.

  • 1X PBS, pH 7.4

    Quality Biological

    PBS (phosphate buffered saline) is a balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Endotoxin and Mycoplasma tested Applications PBS is...

  • Color-Coded pH Buffer Solution Set

    chemForce® (powered by Thomas Scientific)

    Size: 3 x 500 mL Harmonized #: 3822.00.0002|5090 Shelf Life (months): 24 Specific Gravity: 1.00 DOT: Not regulated Appearance: Color coded Physical State: Liquid pH: 4, 7, 10 Flash Point: Not applicable Solubility: Miscible Storage Temperature: Ambient Storage Code: General

  • MOPS, Free Acid

    RPI (Research Products International)

    Molecular Biology Grade Heavy Metals:<5 ppm pka: (20°c) 7.20 ± 0.2 ph range: 6.5-7.9<>

  • Buffer Calibration Solutions

    Any pH measurement is only as accurate as the buffer solution used for calibration. These buffer solutions are traceable to primary standards set by NIST. Buffers 5001A40 through 5001A45 come with a quality assurance certificate per DKD and are particularly useful in quality assurance systems....

  • InLab Storage Solution

    The Mettler Toledo InLab storage solution finds its usage in chemical laboratories and healthcare facilities to ensure the safety of the user. User-friendly design and the strong and efficient construction makes it a must to have at your disposal. Mettler products are intended for sale/shipment...

  • NIST/DIN pH Buffer Solutions

    Single use calibration standards - use one sachet per calibration. Mettler products are intended for sale/shipment within the US only. International customers should contact their local Mettler distributor to place orders for Mettler products.

  • Rainbow Buffer

    Mettler Toledo

    Traceable to NIST Standards Rainbow II Buffer package consists of 30 20 mL sachets of 10 each of 4.01, 7.00 and 10.01 pH solutions. Buffer solutions are traceable to NIST standards and come with a quality inspection certificate which guarantees the stated values and traceability. Rainbow Buffers are...

  • Buffer, Reference Standard, pH 1.00 ± 0.01 at 25°C

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Temperature conversion charts for all Buffers up to pH 11. Multiple sizes to fit your usage and budget...

  • Phosphate Buffer, Standardized

    Composition: Water 95.27%, Potassium Phosphate, Dibasic 2.07%, Sodium Phosphate Dibasic 1.69%, Potassium Dihydrogen Phosphate 0.81%, Ammonium Chloride 0.16% Boiling Point: Approximately 100°C Density: 1.05 Melting Point: Approximately 0°C Color: Colorless liquid Physical State: Liquid pH Range: 7.2...

  • Buffer, pH 7.00, Yellow, Standardized

    Composition: Water 98.73%, Sodium Phosphate Dibasic 0.79%, Potassium Dihydrogen Phosphate 0.47%, Sodium Azide 0.01%, Brilliant Yellow 0% Boiling Point: Approximately 100°C Density: 1 Melting Point: Approximately 0°C Color: Yellow liquid Physical State: Liquid pH Range: 7 Solubility Information:...

  • ACK Lysing Buffer

    Quality Biological

    Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow. Applications ACK Lysing Buffer is used to lyse red blood cells in samples, such as EDTA-treated whole...

  • Buffer, Reference Standard, pH 11.00 ± 0.01 at 25°C

    pH made Easy. Everything you need and want in a pH Buffer. NIST Traceability. Consistent, reliable performance. The tightest specification (+/- 0.01 pH unit) on all buffers. Long Expiration dates. Temperature conversion charts for all Buffers up to pH 11. Multiple sizes to fit your usage and budget...

  • Calibration, Maintenance & Cleaning Solutions

    Milwaukee offers a wide range of calibration, maintenance & cleaning solutions. The use of calibration and cleaning solutions is fundamental for the correct use of electrodes and for obtaining the most accurate and reproducible readings. Often readings are not correct because the sensors have not...

  • Phosphate buffered saline pH 7.4, contains TWEEN® 20, dry powder

    Synonyms: PBS MDL Number: MFCD00131855 Reconstitution: Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); TWEEN ® 20 - 0.05%, pH 7.4, at 25-á°C. Legal Information: TWEEN is a...

  • Traceable® pH Buffer Standards (CRM)

    The most accurate pH Standards available anywhere Certified Reference Material Traceable® Certificate supplied Highest accuracy available Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffers at...

  • Traceable® ph Buffer Standards (CRM), Color Coded

    labForce® (powered by Thomas Scientific)

    The MOST accurate pH Standards available anywhere now color-coded for convenience Traceable® pH Standards, Certified Reference Materials, are 100% compatible with all instruments and probes. Accuracy for the Traceable® Buffer at 25°C is ±0.010 pH—the most precise available....

  • DNAse 1 (RNase-Free).  =2000U/mL

    =2000U/ mL DNase I

  • Optimum Results Filling Solutions

    Thermo Scientific Orion

    For use with ionplus® Sure-Flow® ISE electrodes Little or none of the ion of interest present helps prevent sample contamination Application-specific solution formulation improves response time Small temperature coefficients reduce electrode potential shifts from changing sample...

  • MES Monohydrate

    RPI (Research Products International)

    Buffer for use in plant tissue culture. pKa (20°C): 6.15 pH range: 5.5 - 6.7

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What is nuclease free water?

Nuclease-free water is highly purified water removed of nucleases. This type of water is essential in molecular biology experiments, such as PCR, RNA sequencing, or DNA extraction, where the integrity of nucleic acids is crucial. By using nuclease-free water, scientists ensure that their samples won't be degraded by unwanted enzymes, preserving the accuracy and reliability of their experiments.

Why is nuclease free water used in PCR?

Nuclease-free water is essential in PCR (Polymerase Chain Reaction) to prevent degradation of the DNA template and primers. Nucleases are enzymes that can degrade DNA or RNA, which would compromise the accuracy and success of the PCR process.