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This following method is suggested as a guideline for the use for Western Blot with nitrocellulose or PVDF membranes. The TMB Substrate in 100 mL bottle TMBM-101 and the TMB Precipitating Agent in 10 mL bottle TMBM-102 are used together. Mix 9 ml of the TMB Substrate and 1.0 ml of the TMB...
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Chemical name: Bromine concentrate Chemical synonyms: Bromine solution; Bromide bromate solution Product Brand: Analytical Molecular weight: 159.81 Molecular formula: Br2 UN Number:
6X Concentrated loading dye for molecular biology applications.
MOPS, Free Acid, ULTROL® Grade | CAS 1132-61-2 | Calbiochem | 100GM
Dilute your primary or secondary alkaline phosphatase conjugated antibody or other protein or compound with ALP Conjugate Stabilizer to the desired 1X working concentration determined for your conjugate. ALP Conjugate Stabilizer may be used to dilute conjugates when determining titer. In one step...
Ethylenediaminetetraacetic acid is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. This buffer consists of 0.5 M Trisodium EDTA at pH 8.0. Meticulously prepared using ultra pure reagents dissolved in...
2 x RIPA Buffer IIII with EDTA and EGTA (pH 7.4) 2x concentrate: RIPA buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear...
A buffer solution of HEPES can be prepared by any of several methods. The free acid can be added to water, then titrated with approximately one-half mole equivalent of sodium hydroxide or potassium hydroxide to the desired pH. A simple mixing table for preparing 0.05 M HEPES/NaOH has been published....
TBE is a common buffer used in gel electrophoresis. Supplied as a 1X concentrated solution containing: 89mM Tris Base 89mM Boric Acid 2mM EDTA disodium salt pH (1X solution @ 25°C): 8.3+0.1
Synonyms: Tris(hydroxymethyl)aminomethane hydrochloride buffer solution Density: 1.05 g/mL at 20 °C Other Notes: Buffer component (SET buffer) in the preparation of RNA 1 Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
AAV Affinity Buffer kit (for 5L batch size)
890mM Tris, 890mM Boric Acid, 20mM Ethylenediaminetetraacetic acid (EDTA)
1M Tris-HCl, 100mM Ethylenediaminetetraacetic acid (EDTA)
Certified pH Buffers SPEX CertiPrep pH Buffers are accurate and certified to ISO/IEC 17025:2005 and ISO 17034:2016 requirements. Buffers pH 2 through pH 12 are conveniently packaged in 500 mL bottles. Certified pH buffers are made using high purity starting materials and ASTM Type I Water and are...
Components: HEPES(0.5 M), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.0 ± 0.15 Storage: 2-8°C
CM Galactose Broth minus Leucine, 1000 mL, Sterile. 2 pack.
Chemical name: Ammonium trifluoroacetate Chemical synonyms: Trifluoroacetic acid ammonium salt Product Brand: Analytical Material form: Solid Molecular weight: 131.05 Molecular formula: C2H4F3NO2 Chemical purity: =99.0% (NT) UN Number:
DNA Gel Loading Solution, 5X is prepared according to Sambrook, Fritsch & Maniatis. The purpose of this reagent is to be a tracking dye during electrophoresis. It ensures that DNA samples remain submerged in the wells of the gel, by making them denser than the electrophoresis buffer....
1.5% Tryptone, 0.5% Soytone, 0.5% Sodium chloride, 1.5% Agar
1.0% Tryptone, 2.0% Dextrose
The Bio Basic Glutaraldehyde, 50% Solution with a composition of 50% Glutaraldehyde, 50% Solution, 50% Water, is suitable for use in laboratory use only. It can be stored at a temperature of 2 to 8 degree C.
Chemical name: Iodine solution Product Brand: Analytical Material form: Solution Density (g/cm3): 1.02 Molecular weight: 253.81 Molecular formula: I2 UN Number:
This product is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration. Product consists of 20% (w/v) Sodium Dodecyl Sulfate (SDS). Meticulously prepared using ultra pure reagents dissolved in highly polished...