Biotium

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3' end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number '16' is the...

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits are optimized for labeling 50 ug of antibody with a superior CF® Dye for STORM in 30 minutes with no purification. The kits produce a low degree of labeling that is optimal for super-resolution imaging by STORM. • CF®505 has excitation/emission at...

EvaRuby™ is the first visible red fluorogenic DNA dye for qPCR and HRM, with excitation/emission at 480/610 nm upon binding to dsDNA. It can be combined with fluorescent probes for probe-based qPCR with high resolution melt analysis in the same reaction. 20X in water, 2x1 mL (2000 x 20 uL...

This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently...

Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green...

This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF®660C dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA or PMAxx™ dye and real-time PCR. The kits contain PMA dye, Forget-Me-Not™ qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food...

This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF®430 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green...

Goat anti-mouse IgG1 isotype-specific secondary antibody labeled with CF®405S dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405S dye has...

This product is recombinant Human Annexin V with C-terminal polyhistidine tag expressed from E.coli cells. It is supplied lyophilized and is compatible with dye conjugation to stain phosphatidylserine on the surface of apoptotic cells.

This kit provides calibration buffers and a protocol for producing a range of accurate calcium concentrations for the calibration of fluorescent Ca 2 indicators (1,2). This product contains 50 mL each of components A and B:    Component A (zero free Ca 2 ): Zero mM CaEGTA (10 mM...

TMRM (Tetramethylrhodamine methyl ester, perchlorate) and TMRE are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation. The dyes do not form aggregates in cell membranes and have minimal interaction with membrane proteins. Thus, the transmembrane...

ExoBrite™ CD9 Flow Antibodies are optimized for detection of the exosome marker CD9 in isolated exosomes by flow cytometry. Recommended for instruments optimized for small particle flow. • The 410/450 conjugate has excitation/emission at 408/452 nm for detection in the Pacific Blue™ channel. • The...

Rose-Beta-D-GlcU (6-Chloro-3-indolyl-beta-D-glucuronide,cyclohexylammonium salt), also known as Salmon-beta-D-GlcUA, is similar to X-GlcU, but the product of the substrate is a rose-colored (λ_max ~ 540 nm) precipitate, which is easier to detect against the background of plant...

ExoBrite™ STORM CTB EV Staining Kits are validated for dSTORM imaging of EVs. This optimized formulation of Cholera Toxin B (CTB) binds to components of the EV membrane with bright signal and little to no background. Excitation/emission 681/698 nm.

This product is prepared by labeling high quality goat anti-rabbit IgG (H+L) with CF®532 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange fluorescent CF®532 dye has...

GelRed® Agarose LE is our ultra-pure molecular biology grade LE agarose pre-coated with GelRed® Nucleic Acid Gel Stain. There is no need to handle concentrated fluorescent dye while preparing your gel, for greater convenience and safety. For agarose gels from 0.8% to 2% in TAE or TBE. 50g.

LysoView™ 680 is a unique near-IR live cell lysosome stain for the Cy®5.5 channel. It has excitation/emission at 674/711 nm under acidic conditions. Supplied at 1000X in DMSO, 50 uL.

This product is prepared by labeling monoclonal mouse anti-biotin with CF®750 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF®750 dye has...

This product is prepared by labeling high quality rabbit anti-guinea pig IgG (H+L) with CF®770 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent...

CF®640R-cAMP is a fluorescently labeled cAMP analog. Single molecules of fluorescently labeled cAMP have been imaged bound to the cAMP receptor on the surface of Dictyostelium cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and...

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF®680 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR...

This product is prepared by labeling an affinity-purified chicken anti-mouse IgG (H+L) with CF®633 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

Mix-n-Stain™ antibody labeling kits are a revolutionary antibody labeling technology that allows you to label your antibody with biotin or one of our superior CF® dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody...