Why are 96-well plates used in cell culture?
96-well plates are used in cell culture because they allow for the simultaneous culture and testing of multiple cell samples under identical conditions, facilitating high-throughput screening and efficient use of reagents.
What surface treatments are available for cell culture in 96-well plates?
Surface treatments include coatings with extracellular matrix proteins like collagen, fibronectin, and laminin to promote cell attachment and growth. Other treatments may involve using poly-D-lysine or tissue culture-treated polystyrene for general cell adhesion.
How do I select the appropriate 96-well plate for my cell culture needs?
Selection depends on factors like cell type, assay requirements, and whether you need a treated surface for adherence. Consider the material (e.g., polystyrene), well shape (e.g., flat or round bottom), and color (e.g., clear for microscopy).
What are some common applications of 96-well plates in cell culture?
Applications include drug testing and screening, cytotoxicity assays, proliferation studies, cloning, genetic manipulation studies, and tissue engineering experiments.
How can I prevent edge effects in 96-well plates during cell culture?
Edge effects, caused by temperature and humidity differences, can be minimized by filling perimeter wells with media or buffer, using a humidified incubator, and employing specialized plate designs.
Can 96-well plates be used for 3D cell culture?
Yes, certain 96-well plates are designed for 3D cell culture, often featuring specialized coatings or structures like hydrogels to support spheroid or organoid formation.
What is the optimal volume of media to use in each well for cell culture?
The optimal volume typically ranges from 100 to 200 microliters per well. It's important to use a volume that ensures cells are covered and can access nutrients but not so much that it increases evaporation risks.
How do I ensure sterility in 96-well plates during cell culture experiments?
Use sterile plates and work in a laminar flow hood. Keep plates covered with lids when not in use and handle them with aseptic techniques to prevent contamination.
Are there automated systems compatible with 96-well plates for cell culture?
Yes, many automated systems are designed for use with 96-well plates, including liquid handlers, robotic incubators, and automated microscopes, which streamline cell culture workflows and increase reproducibility.
How do I measure cell viability in 96-well plates?
Cell viability can be measured using assays like MTT, XTT, or alamarBlue. These assays typically involve adding a reagent that changes color or fluorescence in response to metabolic activity, which can be read using a plate reader.
What considerations should be made for imaging cells in 96-well plates?
For imaging, choose clear-bottom plates compatible with your imaging system. Ensure the plate’s material does not interfere with the wavelengths used for fluorescence or luminescence.