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MP Biomedicals

Proteinase K, Recombinant, Reagent Grade, Lyophilized Powder

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Proteinase K Lyophilized Powder, ≥ 30 U/mg, Recombinant (Yeast). Proteinase K, a broad-spectrum serine protease, is first extracted and purified from Engyodontium album. It is named Proteinase K because it can degrade Keratin. The crystallization and molecular structure of Proteinase K indicates it belongs to subtilisin family; whose active site has a characteristic catalytic triple amino acid Asp39-His69-Ser224. Proteinase K has no significant substrate specificity and main cleavage site is the peptide bond at the carboxyl end of the hydrophobic amino acid such as aliphatic or aromatic. Recombinant Proteinase K is derived from yeast based on the optimized gene of Engyodontium album by site-directed mutation. It is purified by chromatography process and with calcium and glycerin as protective agents. Extremely effective on native proteins and can be used to rapidly inactivate endogenous nucleases such as RNases and DNases. This property makes Proteinase K particularly suitable for the isolation of native RNA and DNA from tissues or cell lines.

  • Grade: Molecular Biology
  • Physical Appearance: White lyophilized powder
  • Preparation Method: It is recommended to reconstitute the proteinase K powder with water, add 50% glycerin, or reconstitute with 50% glycerin. Store the reconstituted solution at 2-8°C for long-term storage.
  • Purity: Greater than 95%
  • Molecular Weight: 29 kDa
  • Source: Yeast
  • Specific Activity: Greater than 40 U/mg protein
  • Typical Working concentration: Working concentrations of the enzyme range from 50 to 250 µg/mL.
  • Key Applications: PCR, RNA Purification, DNA Purification, NGS, Sample Preparation

 

  • Grade: Molecular Biology
  • Preparation Method: It is recommended to reconstitute the proteinase K powder with water, add 50% glycerin, or reconstitute with 50% glycerin. Store the reconstituted solution at 2-8°C for long-term storage.
  • Purity: Greater than 95%
  • Molecular Weight: 29 kDa
  • Source: Yeast
  • Specific Activity: Greater than 40 U/mg protein
  • Typical Working concentration: Working concentrations of the enzyme range from 50 to 250 µg/mL.
  • Key Applications: PCR, RNA Purification, DNA Purification, NGS, Sample Preparation

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Product Detail
Thomas No.
CHM00D692
Mfr. No.
0218399001
Pkg. Size
1 g
Description
Proteinase K Lyophilized Powder, >30 U/mg, Recombinant
Grade
Molecular Biology
Pkg. Type
bottle, Plastic Medical Grade
list price/quantitytotal
$0.00
Thomas No.
CHM00D693
Mfr. No.
0218399010
Pkg. Size
10 g
Description
Proteinase K Lyophilized Powder, >30 U/mg, Recombinant
Grade
Molecular Biology
Pkg. Type
bottle, Plastic Medical Grade
list price/quantitytotal
$0.00
$0.00 (0 Items)

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