Polymeric 100 Ã… columns for general reversed phase separations.
- pH stable from 1 to 13
- Better sample recovery than silica-based columns (there are no silanol groups)
- Excellent durability (stable to any concentration of water or organic solvent)
 - Five particle sizes: 5, 7, 10, 12-20 and 30-50 μm
- Eleven column internal diameters: 1.0 to 101.6 mm
- Two column materials: 316 stainless steel and PEEK
- Analytical and semiprep/preparative guard columns
Pack Your Own Columns - Hamilton offers a range of bulk polymer resins for customers who prefer to pack their own column hardware. Chromatographic capacity and efficiency tests are conducted on both bulk and packed column resins to ensure product integrity. Samples from different lots can be purchased for method and process validation.
Superior Sample Recovery - Sample recovery is vital to sample purification. Problems arise when labile samples become irreversibly bound to the silanol groups present on C8 and C18 HPLC columns. Since Hamilton polymers are made entirely of poly styrene-divinylbenzene, there are no silanol groups to cause sample loss. Recovery and quantitation of labile and reactive samples is enhanced. The purification of protected oligonucleotides demonstrates the enhanced recovery of polymer supports. While approximately 50–80% of an oligonucleotide is recovered on a C18 column, the equivalent PRP-1 column recovers 95% or greater of the same sample. Unlike silica-based C8 or C18 columns, PRP-1 has no stationary phase coating. The integral reversed-phase characteristics of the PRP-1 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns.
Protect Your Investment - Hamilton guard columns protect analytical, semi-prep and preparative HPLC columns. They also remove particulate contaminants and highly adsorptive compounds from samples, prolonging column life. The designs are modular and cartridge replacement is easy and tool-free. Cartridge holders for analytical columns are available in stainless steel and PEEK materials. Semi-prep/preparative holders are available only in stainless steel. The design of the cartridge holders is optimized to reduce dead volume and prevent extra column band broadening. Cartridge holders are reusable indefinitely.
> DOWNLOAD the Determination of Flavonoids Application Note from the "Additional Info" tab!