Enrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing
Inactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA/RNA Shield
Remove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS
The Zymo Environ Water RNA Kit simplifies COVID-19 wastewater surveillance by increasing viral enrichment, inactivating pathogens, and improving limits of detection. The use of Water Concentrating Buffer (WCB) promotes the pelleting of viral capsids and protein-associated RNA, increasing SARS-CoV-2 RNA yields by more than 8-fold. DNA/RNA Shield allows for safe handling as it has been validated for inactivation of pathogens including SARS-CoV-2. Finally, the workflow yields highly concentrated, PCR inhibitor-free RNA ready for downstream applications including RT-qPCR, RT-ddPCR, and NGS.
Equipment Needed - Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter/pulverizer (optional)
Format - Bead beating, spin column
RNA Purity - High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations
RNA Storage - RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage
Sample Inactivation - DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures
Sample Input - Water, wastewater, sludge, sewage, biofilms, etc.
Sample Size - Up to 5 mL
Yield - Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample