The material is manufactured from Bovine Plasma collected from USDA licensed abattoirs located entirely within the USA. Animals are inspected pre and post-mortem by a licensed veterinarian and found free of disease.
Plasma undergoes salt fractionation using caprylic acid (sodium salt), heating at 65°C for three hours; alcohol precipitation followed by an acetone wash resulting in a Fraction V powder.
Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Albumin is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Concentration: Protein: 99.0%
Suitability: Suitable for cell culture
Key Applications: Immunoassay, Mammalian Cell Culture
Application Areas: Immunoassays
Product Type: Proteins, Enzymes & Peptides
Protein or Enzyme Type: Albumins
Optical Rotation: [a]259: -61°; [a]264: -63° (Lit.)
Isoelectric point (pI): At 25°C: Fatty Acid Depleted- 5.3, Endogenous Material- 4.7; 4.9 (Lit.)
pH: 7.0 (10% aqueous solution); 6.9 (saline)
Foreign activity: Virus Analysis Bluetongue: Negative Vesicular Stomatitis: Negative
Moisture content: 1.2% By Loss on Drying
NOTES: Sodium: 14 mEq/L 10% aqueous solution
Solubility: Soluble in 40 mg/mL, H2O.
Storage & Handling: Store at 2-8°C.
Certifications: Protease, Peroxidase, Alkaline Phosphatase Free