Agarose IV is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic produces a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.
For improved resolution, we recommend storing the gel at 4°C for 30 minutes before use.
Specifications:
- Moisture: ≤ 7%
- Ash: ≤ 0.25%
- EEO (Electroendosmosis): ≤ 0.12
- Sulfate: ≤ 0.12%
- Clarity (NTU): ≤ 4
- Gel Strength 1% (g/cm²): ≥ 1800
- Gel Strength 1.5% (g/cm²): ≥ 3200
- Gelling Temperature 3% (°C): ≤ 36±1.5
- Melting Temperature 3% (°C): ≤ 88±1.5
- DNase/ RNase Activity: None Detected
- Gel Background: Very Low
Appropriate Agarose Concentrations:
| Gel Concentration | TAE Buffer [1X] |
1.8% | 200-5,000bp |
| 1% | 400-10,000bp |
| 0.8% | 1,000-15,000bp |
| 0.5% | 2,000-20,000bp |
| 0.3% | 3,000-40,000bp |
Migration in the TBE Buffer is slower, therefore lower concentrations can be used to obtain similar separation ranges.
Applications:
- Conventional Electrophoresis
- Pulsed Field Gel Electrophoresis: Due to its higher exclusion limit, large molecules can be separated
- Blotting
- Agarose Bead Preparation
- Cell and Enzyme Immobilization