Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilizes even the most difficult proteins.
Features
- Simple extraction protocol.
- Supplied with yeast specific lytic enzyme preparation.
- Supplied with a strong proprietary chaotropic extraction buffer.
- Suitable for 50 x 60μl yeast cell pellet preparations.
Applications
- Extraction of total proteins from yeast cells.
- Suitable for sample preparation for 2D gel electrophoresis and other applications.