Miscellaneous Protein Sample Prep

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Miscellaneous Protein Sample Prep

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  • CTAB Extraction Solution

    G-Biosciences

    Cetyltrimethylammonium bromide extraction solution for genomic DNA extraction. Applications Genomic DNA extraction buffer solution.

  • Yeast Protein Extraction Buffer Kit

    GE Healthcare

    Yeast Protein Extraction Buffer Kit is a mild detergent-based cell lysis method developed for total extraction of soluble proteins from yeast cells. This kit eliminates the need for glass beads, a common method of mechanical lysis for yeast cells, and is a proprietary improvement on the Zymolyase…

  • Perfect-FOCUS™

    G-Biosciences

    Protein samples loaded on iso-electric focusing (IEF) gels should ideally have low conductivity and be free from agents known to interfere with net protein charge. Protein samples containing interfering agents, including ionic detergents, metal ions, salts, lipids, charged polysaccharides,…

  • Mammalian Protein Extraction Buffer

    GE Healthcare

    Mammalian Protein Extraction Buffer is a 500 ml solution designed for efficient and gentle extraction of biologically active, total soluble proteins from mammalian cultured cells. This buffer is based on organic buffering agents, mild nondenaturing buffer, compositions and can be used for both cell…

  • Sample Grinding Kit

    GE Healthcare

    Sample grinding kit is ready to use for disrupting small tissue and cell samples for protein extraction Disrupts small tissue and cell samples for protein extraction. Uses 1.5 ml microcentrifuge tubes, grinding resin and disposable pestles. Process up to 100 mg of sample per tube in about 10…

  • HOOK™ Cell Surface Protein Isolation

    G-Biosciences

    HOOK ™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK ™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB ™ lysis buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western…

  • Bacterial PE LB™

    G-Biosciences

    Bacterial PE LB™ has been developed for the extraction of soluble proteins and inclusion bodies from bacterial cells. It is a proprietary improvement on the lysozyme based lysis, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released…

  • Column-PROTEIN-Concentrate™

    G-Biosciences

    The Column-PROTEIN-Concentrate™ kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilizes any protein in a low salt buffer…

  • UPPA-PROTEIN-Concentrate™ Kit

    G-Biosciences

    The UPPA-PROTEIN Concentrate™ kit uses our proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1ng/ml into a small volume. Protein precipitation and concentration are not affected by the presence of detergents,…

  • Concentrator Solution

    G-Biosciences

    Concentrator Solution is a novel liquid polymer for the rapid concentration of dilute protein solutions with zero loss, using dialysis. Simply transfer your dilute protein solution to a dialysis bag or dialysis device*, such as our patented Tube-O-DIALYZER ™ and dialyze against the…

  • OrgoSol-PROTEIN-Concentrate™

    G-Biosciences

    The OrgoSol-PROTEIN-Concentration™ kit precipitates protein with a proprietary solvent buffer, OrgoSol™. The OrgoSol™ buffer has been specifically developed for efficient precipitation of protein solutions with minimal disruption to the protein structure and therefore maintains…

  • FOCUS™ Signal Protein

    G-Biosciences

    Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including…

  • Bis-Tris Gel Buffer, 4X

    Amresco

    Bis-Tris Gel Buffer, 4X is used to prepare polyacrylamide gels for optimal separation of small- to medium-sized proteins under denaturing conditions. The neutral pH environment of the gel prepared with Bis-Tris Gel Buffer minimizes protein modifications. Bis-Tris Gel Buffer, 4X can be used to…

  • HOOK™ Sulfo-NHS-Biotin (Micro)

    G-Biosciences

    HOOK™-Sulfo-NHS-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of which up to 35…

  • PAGE-Perfect™

    G-Biosciences

    Remove Electrophoretic Contaminants for Improved Protein Resolution and Publication Quality Gels; Ideal for Difficult or Dilute Samples Many lysis buffers and reagents used in sample preparation are incompatible with routinely used electrophoretic analysis. The presence of contaminants, or…

  • LDS Sample Loading Buffer, Non-Reducing (4X)

    Amresco

    LDS Sample Loading Buffer, Non-reducing (4X) is used to prepare protein samples for denaturing polyacrylamide gel electrophoresis using Bis-Tris and Laemmli gels. Mixing LDS Sample Loading Buffer with protein samples only denatures the proteins without reducing them. Reducing agents such as DTT or…

  • PE LB™ for Yeast

    G-Biosciences

    Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make…

  • PAGE-Optimizer

    G-Biosciences

    PAGE-Optimizer™ is a unique, spin column for the preparation of samples for SDS-PAGE.The columns contain a proprietary separation matrix that removes contaminants such as salts, detergents, cellular agents and other common buffering agents. PAGE-Optimizer™ helps eliminate smeared…

  • PE LB™ for Tissue

    G-Biosciences

    Tissue-PE LB™ has been developed for extraction of total soluble protein from animal tissues.Tissue- PE LB™ is based on an organic buffer, which utilizes a mild non-ionic detergent, and a proprietary combination of various salts and agents to enhance extraction and stability of…

  • FOCUS™ Global Fractionation

    G-Biosciences

    FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent…

  • FOCUS™ Insect Proteome

    G-Biosciences

    Extracts and solubilizes nearly all of the proteins from insect cell cultures (i.e. Sf9 and Sf21), including membrane as well as soluble proteins, using a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Features Single step extraction protocol. …

  • Fraction-FOCUS™

    G-Biosciences

    2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins, however the greatest challenge in protein identification is achieving suitable resolution of proteins. The high dynamic range of a species' proteome means that the more abundant proteins mask the…

  • FOCUS™ Mammalian Proteome

    G-Biosciences

    Extracts and solubilizes nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilize even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.…

  • Concentrator for Tube-O-Dialyzer™

    G-Biosciences

    Concentrator Powder is a high molecular weight polymer which will not migrate across the dialysis membrane. Simply transfer your dilute protein solution to a dialysis bag or dialysis device, such as our patented Tube-O-DIALYZER ™ and then cover the membrane with Concentrator Powder.…

  • HOOK™ IgG Biotinylation (Amine)

    G-Biosciences

    Simple Antibody Biotinylation The HOOK™ IgG Biotinylation (Amine) kit is designed for the efficient biotinylation of IgG molecules by immobilizing the IgG molecules on a solid support (see figure 1). IgG molecules contain a histidine rich region in their Fc region and this feature…

  • FOCUS™ Plant Proteome

    G-Biosciences

    Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilizes nearly all of the proteins from plants, including membrane as well as…

  • UPPA™-I & II Pack

    G-Biosciences

    Our Universal Protein Precipitation Agent, commonly known as UPPA™, has been specifically designed for the quantitative concentration of protein solutions as dilute as 1ng/ml into a small volume. Protein concentration and precipitation is not affected by the presence of detergents,…

  • HOOK™ NHS-dPEG4 -Biotin (Micro) Kit

    G-Biosciences

    HOOK™-NHS-dPEG™4-Biotin reacts with protein primary amines. Amines, lysine ε-amines and N-terminal α-amines, are the most abundant group in protein molecules and represent the most common target for biotinylation. For example, BSA contains 59 primary amines, of…

  • Bacterial PE LB™ in Phosphate Buffer

    G-Biosciences

    Bacterial PE LB™ in Phosphate buffer is a variation of Bacterial PE LB™ buffers only in phosphate buffer. It is used preferably for extraction of soluble proteins and soluble recombinant proteins from bacterial cells. Bacterial PE LB™ in Phosphate buffer contains a mild…

  • FOCUS™ Membrane Proteins

    G-Biosciences

    FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90%…

  • FOCUS™ Bacterial Proteome

    G-Biosciences

    Specifically designed for bacterial research and supplied with bacteria specific reagents. Extracts and solubilizes nearly all of the proteins from E.coli , including membrane as well as soluble proteins. Extraction is based on the gentle lysis of bacterial cells with LongLife ™ Lysozyme…

  • FOCUS™ Glycoprotein

    G-Biosciences

    FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins. FOCUS™ Glycoprotein kit isolates glycoproteins from complex biological solutions using spin columns that contain lectin (Concanavalin A)…

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