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bioWORLD Enzymes & Inhibitors

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  • Alkaline phosphatase

    bioWORLD

    1,000 U Conjugation Grade Ammonium Sulfate Suspension Source: Calf Intestine Adenosine Deaminase: <=0.001% Concentration (37 C): >7500U/ml Phosphodiesterase: <=0.001% DNase, RNase, and Protease: None Specific activity (37 C, pH 10.5, DEA): >1500U/mg

  • AEBSF (4-(2-Aminoethyl)benzenesulfon

    bioWORLD

    Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin and other related thrombolytic enzymes. Less toxic substitute for PMSF. Specific and irreversible inhibitor of serine proteases. Synonym: 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride pKb: 9.19 (Predicted) …

  • X-Glu

    bioWORLD

    Also known as 5-Bromo-4-Chloro-3-indolyl-β-D-Glucopyranoside. A substrate for Beta-glucosidase that renders an intense indigo-blue chromophore (615 nm) upon enzymatic action. Used as an indicatior-probe in histochemistry and in culture media to detect beta-Glucosidase positive organisms.

  • DNase I (bovine pancreas)

    bioWORLD

    DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I…

  • Peroxidase (horseradish)(HRP)

    bioWORLD

  • Proteinase K

    bioWORLD

    A serine proteinase used to inactivate RNAse and DNAse in RNA and DNA isolation protocols. Useful in the study of membrane and protein structure. Proteinase K is a highly active protease (MW 28,500 Da) isolated from the fungus Tritirachium album . The enzyme exhibits broad specificity on…

  • Cellulase (Onozuka R-10)

    bioWORLD

    From Trichadema Viride. Enzyme mixture often used in combination with Macerozyme R-10 (M22010) for isolation of plant protoplasts. One unit of Cellulase will liberate 1.0 µmole of glucose from carboxymethyl cellulose. Lyophilized powder Optimum pH range: 4-5

  • X-Gal-IPTG, Ready- to-use, Spray

    bioWORLD

    X-Gal-IPTG, Ready- to-use, Spray Stable X-gal/IPTG non-toxic solution For use in blue/white colony screening in expression studies Does not contain toxic DMF and DMSO Contains 40 mg/ml of IPTG (Isopropyl-β-D-Thiogalactopyranoside) and 40 mg/ml of X-gal…

  • Trypsin, TPCK-treated

    bioWORLD

    Protease which cleaves specifically at the -COOH side of arginie and lysine. TPCK-treated Trypsin is a serine endopeptidate Trypsin is treated with TPCK to inactivate any remaining chymotryptic activity This type of trypsin is modified by acetyltion of the sigma-amino groups of lysine…

  • Glucose oxidase

    bioWORLD

    Molecular Biology Grade Specific activity (25° C): ≥95U/mg Solubility (1%, water): Clear and haze-free Glucose Oxidase: Catalase ratio >100:1 Unit Definition: The amount of enzyme which causes the oxidation of 1 micromole of glucose per minute at 25 C, pH 7.0 50 ku

  • D-Luciferin (free acid)

    bioWORLD

    Also known as 4,-5-Dihydro-2-(6-hydroxyl-2-benzo-thiazoyl)-4-thiazole-carboxylic acid. Used with firefly luciterase in bioluminiscence for the determination of ATP.

  • Trypsin (bovine)

    bioWORLD

    A serine peptidase that cleaves at internal peptide bonds on the carboxyl-side of arginine and lysine residues. USP Grade Activity (min.) at 25 C >2,500U/mg Chymotrypsin 5.0% Unit Definition: The amount of enzyme which causes an increase in absorbance of 0.003 per minute at 25 C…

  • Trypsin 1:250

    bioWORLD

  • T4 DNA ligase

    bioWORLD

    T4 DNA Ligase catalyzes the forming of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with blunt or cohesive-end termini. This ligase repairs single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids but has no activity on…

  • Malate dehydrogenase

    bioWORLD

    Malate Dehydrogenase, Ammonium Sulfate Suspension

  • Zymolyase

    bioWORLD

    Yeast lytic enzyme

  • Beta-galactosidase

    bioWORLD

    Enzyme found in lac+ bacterial cells that catalyzes the cleavage of -linked terminal galactose-containing substrates. Lyophilized powder Source: E. coli Conjugation grade Activity (37°C):800-1000U/mg protein Unit definition: One unit will hydrolyze 1.0 μmole of…

  • Pepsin 1:3000 (porcine source)

    bioWORLD

  • BCIP

    bioWORLD

    Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue,…

  • Lysozyme

    bioWORLD

    Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.

  • Cicer aretinum (Chickpea) - lectin

    bioWORLD

    Isolated from Chick Pea Supplied as lyophilized powder

  • Pectinase

    bioWORLD

    Also known as Poly-(1,4-a-D-galacturonide)glycanohydrolase. Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

  • WGA (Wheat Germ Agglutinin) Lectin

    bioWORLD

    Lectin isolated from Triticum vulgaris (wheat germ). Wheat germ agglutinin (WGA) is used for binding to variety of receptors, agglutination reaction and used as ligand for purification of specific membrane proteins.

  • AP-blue, AP-yellow

    bioWORLD

    2 component microwell phosphatase substrate with excellent performance characteristics and a wide working range. Develops blue reaction product measurable at 490, 550, or 620 nm.

  • S1 Nuclease

    bioWORLD

    The S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides. It is more active on DNA than on RNA. The S1 Nuclease exhibits 3'-phosphomonoesterase activity. The enzyme is a glycoprotein with MW of 29 kD monomer. Source Aspergillus oryzae

  • Chymotrypsin A

    bioWORLD

    A serine endopeptidase that cleaves peptides on the carboxyl-side of aromatic residues.

  • FireTag AP

    bioWORLD

    Enables antibody or protein labeling with alkaline phosphatase (AP) in less than 3 hours, saving you several hours over standard maleimide/thiol methods. AP is supplied ready-to-use, preactivated and requires no activation or purifi cation. Suffi cient reagents are provided to conjugate 3 samples,…

  • ABTS (2,2'-Azino-bis(3-ethylben

    bioWORLD

    One Component Microwell Peroxidase Substrate with excellent performance characteristics, good stability and wide working range. Develops blue-green color measurable between 405 nm and 410 nm.

  • Trypsin 1:300

    bioWORLD

  • Erythrina (coral tree) - Lectin

    bioWORLD

    Lectin isolated from Erythrina cristagalli (coral tree) seeds. Agglutination activity is expressed in mg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 µg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of…

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