Enzymes & Inhibitors

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G-Biosciences Enzymes & Inhibitors

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  • Immobilized Aprotinin

    G-Biosciences

    Aprotinin is a monomeric globular protein which inhibits several serine proteases including trypsin, chymotrypsin, plasmin and kallikrein. Furthermore, its action on kallikrein leads to inhibition of formation of factorXIIa. The binding of aprotinin to proteases is reversible with dissociation of…

  • Taq DNA Polymerase

    G-Biosciences

    Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus. The molecular weight of the recombinant protein is 94kD.. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The…

  • A Chemically Modified, TPCK treated, Affinity Purified Trypsin Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues, and typically, there is little to no cleavage at arginyl-proline and lysyl-proline…

  • Recombinant human caspases 1-3 and 6-10 are supplied lyophilized in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves 1nmol of a specific caspase substrate per hour at 37ºC in a reaction solution containing 50mM HEPES (pH7.2), 50mM…

  • 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride Specificity: Specific irreversible inhibitor of serine proteases, including chymotrypsin, kallikrein, plasmin, thrombin and trypsin. A stable non-toxic alternative to PMSF. Solubility: Soluble in water Features …

  • Taq Plus Mix [2X] is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase , dNTPs , Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to…

  • Trypsin

    G-Biosciences

    Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. Typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. This trypsin is purified from bovine pancreas, 1X…

  • Camptothecin [2mM]

    G-Biosciences

    4-ethyl-4-hydroxy-1H-pyrano[3’,4’:6,7]indolizino[1,2-b] quinoline-3,14-(4H,12H)-dione A cytotoxic quinoline alkaloid that inhibits the DNA enzyme topoisomerase I (Topo I). Supplied as 1ml 2mM Features Synonym: CPT CAS Number: 7689-03-4 Linear Formula: …

  • Firefly luciferase is a unique photoprotein used in bioluminescence for high sensitive detection and quantification of ATP and as a reporter for studying gene function and regulation.Luciferase (Firefly, Recombinant) is recombinant 62 kDa protein expressed in E. Coli. Click here to request…

  • Dexamethasone [10mM]

    G-Biosciences

    (9-fluoro-11β,17,21-trihydroxy-16a-methylpregna-1,4-diene-3,20-dione) A potent and stable glucocorticoid steroid hormone that is often used as an anti-inflammatory and anti-rheumatic drug. The mechanism of action is not fully understood, however it is thought that it may inhibit…

  • Enzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. A wide variety of enzyme preparations in a ready-to-use format are offered. LongLife™ enzyme…

  • Trypsin is a serine protease that cleaves peptides on C-terminal end of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7.0-8.0. Trypsin is inhibited by serine protease inhibitors including TLCK (N-p-tosyl-L-lysine chloromethyl ketone), PMSF (phenylmethanesulfonyl…

  • Staurosporine

    G-Biosciences

    Staurosporine is a strong inhibitor of phosphalipid/Ca2+ dependent protein kinase and platelet aggregation. It is most commonly used as an inducer of apoptosis in several mammalian cell types. It is also used to study role of protein kinases in signal transduction as it inhibits other protein…

  • Proteinase K

    G-Biosciences

    Proteinase K (also protease K, endopeptidase K, peptidase K or Tritirachiumalkaline phosphatase) (EC 3.4.21.64) is a non-specifc, broad spectrum serine protease that is isolated from the saprophytic fungus Tritirachium album. Proteinase K is routinely used for the purification of target…

  • A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases. An optional EDTA solution is provided for…

  • Pepstatin

    G-Biosciences

    Isovaleryl-Val-Val-AHMHA-Ala-AHMHA where AHMHA= (3S, 4S)-4-amino-3-hydroxy-6-methyl-heptanoic acid Specificity: A potent inhibitor of various aspartic proteases, including cathepsin D, renin, pepsin, bacterial aspartic proteases and HIV proteases. Solubility: Soluble in DMSO or methanol …

  • LongLife™ DNase

    G-Biosciences

    Enzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. A wide variety of enzyme preparations in a ready-to-use format are offered. LongLife™ enzyme…

  • PhosphataseArrest™

    G-Biosciences

    The PhosphataseArrest™ phosphatase inhibitor cocktails are 100X concentrated, ready-to-use solutions that are simply added to your extraction buffers or samples. PhosphataseArrest™ is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most…

  • Apoptotic Inducer Set

    G-Biosciences

    Ready-to-use high quality apoptosis inducers allow users an easy tool for induction of apoptosis in cultured cells. These stabilized reagent solutions have a long storage life and are easy to use. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone,…

  • LongLife™ Zymolyase®

    G-Biosciences

    Enzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. G-Biosciences offersa wide variety of fresh, ready-to-use enzyme preparations. Our high performance…

  • Cycloheximide [100mM]

    G-Biosciences

    (4-{(2R)-2-[(1S,3S,5S)-3,5-dimethyl-2-oxocyclohexyl]-2-hydroxyethyl}piperidine-2,6-dione) An inhibitor of protein synthesis by interfering with the translocation step (movement of two tRNA molecules and mRNA on ribosome) in protein synthesis, thus blocking translational elongation. …

  • Recom ProteaseArrest™

    G-Biosciences

    A broad range, bacterial, 100X concentrated, ready-to-use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Inhibits bacterial serine, cysteine, metallo-and other bacterial specific proteases including…

  • SG-Carboxypeptidase B (Recombinant)™is the rat carboxypeptidase B expressed in E. coli. Carboxypeptidase B specifically hydrolyses basic amino acids including lysine, arginine and histidine from the C-terminal end of polypeptides. It is used in sequencing protein and peptides. Other…

  • Pfu Mastermix [2X]

    G-Biosciences

    Pfu DNA Polymerase Mastermix [2X] is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix…

  • Features Synonyms: α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, Phenylmethylsulfonyl fluoride CAS Number: 329-98-6 Molecular Formula: C₇H₇FOâ‚‚S Molecular Weight: 174.19 g/mol Activity: >99.9% …

  • SG-Chymotrypsin (Human, Recombinant)™ is recombinant human chymotrypsin expressed in E. coli and purified by HPLC method. Chymotrypsin hydrolysis at the carboxyl side of aromatic amino acid residues including Tyrosine, phenylalanine and Tryptophan. Cleavage occurs at lower rate at…

  • A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases. ProteaseArrest Family Our ProteaseArrest™ is a highly efficient, liquid format general protease…

  • Protease Inhibitor Set

    G-Biosciences

    Contains 12 ready-to-use individual protease inhibitors for characterization of protease activity. Each set contains the following protease inhibitors. See individual inhibitors for their specificities and other information.: AEBSF ALLN Antipain, dihydrochloride Aprotinin …

  • A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. Bacterial ProteaseArrest ™ inhibits bacterial serine, cysteine and other bacterial specific proteases including aminopeptidases and aspartic proteases. An optional EDTA solution is provided for enhanced…

  • ProteaseArrest™

    G-Biosciences

    ProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution. The ProteaseArrest™ 100X solution format is suitable for small, analytical sample applications, as >95% inhibition is achieved by adding 10µl…

  • Pfu Polymerase

    G-Biosciences

    Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min…

  • Immobilized Trypsin

    G-Biosciences

    Immobilized Trypsin is TPCK Treated Trypsin immobilized on 4% agarose that eliminates the contamination of protein digests by the trypsin. The immobilized trypsin is readily removed by separating the agarose from the digestion solution. Trypsin is a serine endopeptidase that specifically…

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