Nonidet P-40

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Nonidet P-40
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  • Nonidet® P-40 Substitute Nonidet® P-40 Substitute is a non-ionic detergent that is provided for research purposes or as a 10% proteomic grade solution. Proteomic Grade:DG001, DG002, DG501, DG514 Research Grade: 786-511, 786-512 Proteomic Grade Detergent Solutions …

  • A nonionic surfactant useful in isolating and purifying membrane proteins A non-ionic nondenaturing detergent used for solubilizing proteins Specifications Can be used as a substitute for octylphenoxypolyethoxyethanol CMC: 0.29 HLB Number 13.1 Reagent Grade Refractive Index:…

  • Many commercial grade detergents contain elevated levels of sulfhydryl oxidizing agents, peroxides, salts and carbonyl compounds (Figure 1). The proteins that are isolated with these detergents are highly susceptible to contaminating peroxides and carbonyls. The peroxides will oxidize proteins and…

  • RPI (Research Products International)

    Laboratory reagent grade. Nonidet P-40 is a nonionic, non-denaturing detergent.

  • Boston BioProducts

    Components: Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Sodium Dodecyl Sulfate (SDS)(0.2%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) …

  • Boston BioProducts

    Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Nonidet P-40 Substitute(1%) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 Storage: 2-8°C

  • Boston BioProducts

    Components: Tris Base / Tris-HCl(50 mM), Sodium Chloride(150 mM), Nonidet P-40 Substitute(1%), Sodium Deoxycholate(0.5%), Sodium Dodecyl Sulfate (SDS)(0.1%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 …

  • Boston BioProducts

    Components: Tris Base / Tris-HCl(250 mM), Sodium Chloride(750 mM), Nonidet P-40 Substitute(5%), Sodium Deoxycholate(2.5%), Sodium Dodecyl Sulfate (SDS)(0.5%), Water(Rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 7.4 ± 0.15 (1X) …

  • Boston BioProducts

    Components: Sodium Dodecyl Sulfate (SDS)(0.2%), Tris Base / Tris-HCl(100 mM), Sodium Chloride(300 mM), Nonidet P-40 Substitute(2%), Sodium Deoxycholate(1%), Water(rest) Method: Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. pH: 8.0 ± 0.15 (1X) …

  • … (C2H4O)nC14H22O CAS No.: 9036-19-5 Quality: Chemically indistinguishable from Nonidet P-40, which is no longer commercially available. Caution: May develop turbidity or sediment during storage. A clear liquid can be obtained on heating…

  • …40°C. Other Notes Nonionic, non-denaturing detergent Quality Chemically indistinguishable from Nonidet P-40, which is no longer commercially available. Legal Information IGEPAL is a registered trademark of Rhodia Operations; Nonidet is a trademark of Shell Chemical Co.

  • …1.06 g/cm 3 at 25 °C(lit.) Application: For solubilizing and isolation of membrane proteins. Quality: Chemically indistinguishable from Nonidet P-40, which is no longer commercially available. Caution: May develop turbidity or sediment during storage. A clear liquid can be obtained on…

  • …mM MgCl 2 , 500 mM KCl and 1% Triton X-100, pH 8.3 (4°C). Enzyme Storage Buffer: 20 mM Tris-HCl, 1 mM DTT,0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol(v/v), pH 8.0 (4°C) Applications The applications of Green Taq DNA Polymerase include the…

  • …one 10ml vial of 10% aqueous solutions of : Triton® X-100 Triton® X-114 Tween® 20 Tween® 80 Nonidet® P-40 Substitute Brij® 35 Brij® 58 and 1gm of: CHAPS NDSB 201 Features Low peroxide contamination…

  • G-Biosciences

    …carries the pol gene for Thermus aquaticus. STORAGEBUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mMDTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol. 10X PCR BUFFER 100mM Tris-HCL pH8.8, 500mM KCl, 16mM MgCl2,1% TritonX-100…

  • G-Biosciences

    …Grade Detergent Solutions offered include: Triton® X-100 , Triton® X-114 , Brij® 58 , Tween® 20 , Tween® 80 and Nonidet® P-40 Substitute . A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic…

  • G-Biosciences

    …Detergent Solutions offered include: Triton® X-100 , Triton® X-114 , Brij® 35 , Brij® 58 , Tween® 20 and Nonidet® P-40 Substitute . A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic…

  • G-Biosciences

    …Proteomic Grade Detergent Solutions offered include: Triton® X-114 , Brij® 35 , Brij® 58 , Tween® 20 , Tween® 80 and Nonidet® P-40 Substitute . A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic…

  • G-Biosciences

    …Proteomic Grade Detergent Solutions offered include: Triton® X-100 , Brij® 35 , Brij® 58 , Tween® 20 , Tween® 80 and Nonidet® P-40 Substitute . A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic…

  • G-Biosciences

    …Detergent Solutions offered include: Triton® X-100 , Triton® X-114 , Brij® 35 , Brij® 58 , Tween® 80 and Nonidet® P-40 Substitute . A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic…

  • G-Biosciences

    …Grade Detergent Solutions offered include: Triton® X-100 , Triton® X-114 , Brij® 35 , Tween® 20 , Tween® 80 and Nonidet® P-40 Substitute . A variety pack is also available, perfect for trial and optimization. The pack contains one 10ml vial of all seven proteomic…

  • …Extraction Buffers are experimentally optimized concentrations of critical, buffering and stabilizing agents, including urea, thiourea, Nonidet® P-40, CHAPS and sulfobetaines. The FOCUS™ Extraction Buffers are designed to produce optimal protein extraction and improved spot resolution…

  • G-Biosciences

    …high-fidelity DNA synthesis. STORAGEBUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol. 10X Pfu BUFFER 200mM Tris-HCl (pH8.8), 100mM KCl, 100mM…

  • …Buffers are experimentally optimized with proprietary technologies and critical buffering and stabilizing agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulfobetaines. Our 2D Sample Extraction Buffers are designed to produce optimal protein extraction and significantly improved…

  • G-Biosciences

    …0.35mg/ml Deoxycholate 0.1% Methanol 10% Tween® 20 0.03% DMSO 10% MES 0.7M Urea 3M DNA 1mg/ml Nonidet® P-40 0.5% Features Sensitivity: Linear responses over the range of 0.5µg-50µg protein Flexible…

  • …thiocyanate, 10mM EDTA 6M urea, 2M thiourea, 4% CHAPS 6M urea, 2M thiourea, 2% CHAPS, 2% ND SB 201 6M urea, 2M thiourea, 4% Nonidet® P-40 6M urea, 2M thiourea, 2% CHAPS, 2% SB 2 10 Features Linear response 0.5µg-50µg protein Small sample…

  • …for the most applications, but—depending on the membrane and on the antibody used—different detergents (like SDS, Triton X-100 and Nonidet P40 and detergent concentrations from 0.01 to 1% may lead to better results. •  Blocking solution (1%) Dilute 10 ml Western Blocking…

  • G-Biosciences

    … Triton X-100, 2% >99 >90 >91 >92 >93 Triton X-114, 2% >96 >99 >98 >97 >91 Nonidet P-40, 1% >96 >93 >95 >91 >91 Brij 35, 1% >99 >98 >99 >97 >91 SDS, 2.5% >99 >96…

  • G-Biosciences

    … CHAPS 2% DTT 1M CHAPSO 2% CHAOTROPES Nonidet ® P-40 2% Guanidine.HCl 6M SDS 2% Urea 6M …

  • G-Biosciences

    …amplification is 7.5x10 -5 per nucleotide per cycle. STORAGE BUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl2, 1mM DTT, 0.1% Nonidet® P-40,0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol. 10X PCR BUFFER 100mM Tris-HCl (pH8.8), 500mM potassium chloride, 1%…

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