Imidazole is a chelator for the binding of various divalent cations. Imidazole is a highly polar, planar 5 membered ring. It is amphoteric in nature. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins and can also be used in reverse staining of…

Hydroxyurea is a inhibitor of DNA synthesis in vivo, and an antiviral and antineoplastic agent which is S-phase specific. A ribonucleotide reductase inhibitor - inhibits by forming a free radical nitroxide that binds a tyrosyl free radical in the active site of the enzyme. Induces fetal hemoglobin…