Imidazole is a chelator for the binding of various divalent cations. Imidazole is a highly polar, planar 5 membered ring. It is amphoteric in nature.
Imidazole can be used for the elution of histidine containing proteins from divalent cation resins and can also be used in reverse staining of SDS-PAGE gels for detection of proteins . Imidazole is used to elute tagged proteins bound to Ni ions attached to the surface of beads in the chromatography column. Synthetic imidazoles are present in many fungicides and antifungal, antiprotozoal, and antihypertensive medications.
Excellent for buffer use in pH range 6.2-7.8.
Key Applications: Preparation of Buffers
Product Type: Biochemicals
Chemical Class: Biochemicals
Density: 500 - 600 kg/m3 (bulk density) (Lit.)
Auto Ignition: 480°C (Lit.)
Boiling Point: 268°C (Lit.)
Melting Point: 86 - 94°C
Vapor Pressure: .003 hPa at 20°C (Lit.)
Presentation: White Crystaling Powder
pH: ≥7 (1% aq soln)
Solubility: Freely soluble in water, alcohol, ether, chloroform, pyridine; slightly soluble in benzene; very sparingly soluble in petroleum ether.
Storage & Handling: Store at Room Temperature.