Vectors for adenoviral gene delivery and tandem affinity purification Purify interacting proteins from a broader range of mammalian cells;Adenoviral gene delivery for high titers and improved yields Recombinant adenoviruses can infect either dividing or non-dividing cells Tandem…

Get easy detection of expression with humanized hrGFP, qualitatively assess transfection efficiency Qualitatively assess the transfection efficiency of the producer cell line Determine the viral titer using fluorescence microscopy or by fluorescence activated cell sorting (FACS) Use…

Easily induce and express phosphorylated proteins, which can be used to screen expression libraries, to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target. Competent cell lines for inducible phosphoprotein expression TKB1 Competent Cells are derived…

InterPlay Mammalian TAP System provides proven purification of interacting proteins Recover intact interacting protein partners without protease digestion, as SBP and CBP elute easily from their affinity resins Exceptionally pure protein for downstream analysis;Includes all vectors,…

Recommended for tittering adeno-associated recombinant virus stocks for the AAV Helper-Free system Recommended for tittering adeno-associated recombinant virus stocks for the AAV Helper-Free system AAV-HT1080 cells are free of microbial contamination, as determined by sterility culture…

The AD-293 cell line simplifies the process of producing recombinant adenovirus particles. For use with Agilent InterPlay Adenoviral TAP System and AdEasy vectors Improved adherence to tissue culture dishes over standard HEK293 cells Simplifies the process of producing recombinant…

Recombination proficient bacterial strain for production of adenovirus plasmid DNA 300% more recombinants with specialty competent cells Recombination proficient bacterial cell strain Infects dividing and non-dividing cells BJ5183 electroporation competent cells are a…

Tackle two common bacterial gene expression hurdles: protein insolubility and codon bias Low-growth temperatures enhance protein folding and solubility Dramatically improves expression for AT-rich or GC-rich genomes when codon bias is a problem Compatible lac, tac , and trc …

Address bacterial gene expression hurdle of protein insolubility Low-growth temperatures (or cold-adapted technology) enhance protein folding and solubility Cells are derived from the high-performance Agilent BL21-Gold competent cells;Feature chaperonins Cpn60 and Cpn10 to facilitate proper…

BL21(DE3) competent cells provide nontoxic protein expression and high transformation efficiency. Cloning directly in the strain can save you two days of work on subcloning procedures BL21-gold competent cells feature the high transformation efficiency phenotype (Hte) and have the gene…

BL21 competent cells are an all-purpose strain for high-level protein expression and easy induction. High-level protein expression and easy induction Can be used with non-T7 RNA polymerase protein expression systems Allows the tightest control of protein expression for extremely…

Dramatically improve protein expression in E. coli by overcoming codon bias Suitable for expressing proteins from all species, including mammals;Ideal for difficult protein expression, especially when codon bias is a problem;Increases transformation efficiency with Hte phenotype;Derived from…

AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in helper-free systems. AAV-293 cells are derived from the commonly used HEK293 cell line but produce higher viral titers. HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5…

Reduce the time is takes to generate a recombinant adenovirus for gene delivery and expression by several weeks 300% more recombinants with specialty competent cells with the AdEasy system;Infects both dividing and non-dividing cells Human proteins expressed using this system are abundant…