Added to fluorescence-based qPCR assays to compensate for non-PCR related fluorescence variations. 1 mM, for controlling the final dye concentration Comes in 10 vials of 100 µL Added to fluorescence-based qPCR assays The passive reference dye can be added to fluorescence-based…

This special formulation of cloned Pfu DNA polymerase increases PCR product yields up to 19 kb. Robust performer with very low error rate (six times more accurate than Taq) Contains ArchaeMaxx factor for high-performance up to 19 kb for gDNA and 15 kb for vector DNA Available as a…

Superior yields with extension times as short as 15 sec/kb, even with genomic DNA targets up to 23kb. Superior yield for routine PCR applications and difficult/GC-rich targets Fast cycling times with 15 sec/kb extension rate;Accurately amplifies up to 23 kb genomic DNA targets …

The industry standard for high-fidelity PCR, achieve extreme accuracy with downstream apps. Highest fidelity (1 error/2.5 million bp) for the most reliable, error-free PCR Fast extension for 70-80% quicker time-to-results Accurately amplifies up to 19 Kb genomic DNA targets …

High-quality polymerases for difficult to amplify DNA targets. Proofreading DNA polymerase isolated from Pyrococcus furiosus. Ideal choice for many techniques requiring high-fidelity DNA synthesis by PCR;Blunt ends Agilent’s range of Pfu DNA Polymerases includes Pfu DNA…

Increases specificity and yield of primary PCR amplification products. Increases yield and specificity of primary PCR amplification products Minimizes formation of poorly matched primer-template complexes Add to genomic PCR reactions to destabilize many mismatched primer-template…

SURE 2 competent cells are ideal for routine cloning of DNA with secondary structures. Lack components of the pathways that catalyze the rearrangement and deletion of nonstandard secondary and tertiary structures Allow for blue-white color screening Replicating eukaryotic DNA in…

qPCR Brilliant II SYBR MM These fast qPCR kits come in master mix format, including hotstart enzyme Low-abundance RNA;Amplification and detection from total RNA, poly(A+), and synthetic RNA samples The Brilliant II qPCR SYBR master mix includes the components necessary to carry out PCR…

A 50-kDa protein that specifically inhibits common eukaryotic RNases Only 25 to 40 U is required for a standard transcription reaction. Monitored for endogenous exonuclease and endonuclease activity. Determined to be free of contaminating RNases by incubating RNA transcripts. …

Brilliant III Ultra-Fast SYBR qRT-PCR Master Mixes utilize a novel faster-activating hot start method Provides specificity by utilizing a novel faster-activating hot start method that minimizes formation of primer-dimers and other off-target reactions Pre-blended formulations are compatible…

Get easy detection of expression with humanized hrGFP, qualitatively assess transfection efficiency Qualitatively assess the transfection efficiency of the producer cell line Determine the viral titer using fluorescence microscopy or by fluorescence activated cell sorting (FACS) Use…

Vectors for adenoviral gene delivery and tandem affinity purification Purify interacting proteins from a broader range of mammalian cells;Adenoviral gene delivery for high titers and improved yields Recombinant adenoviruses can infect either dividing or non-dividing cells Tandem…

Achieve consistent high specificity during qRT-PCR detection of miRNA in real time Achieve accurate real-time PCR quantification for miRNA. A high-specificity reagent system that delivers consistent, sensitive and reproducible results. The kit elongates miRNAs in a polyadenylation…

Application-ready RNA available in economical and convenient 25 µg packs. Extensive quality control ensures high-quality RNA Eliminates tedious, time consuming RNA isolation procedures Application ready for real-time RT-PCR, miRNA detection, and Northern blot analysis Matched…

Phage display libraries from electroporation-competent cells with high transformation efficiency. Just thaw, combine with DNA, and electroporate to get started quickly Efficiency of > 1 x 10^10 transformations/µg of pUC18 DNA Kit size 5 x 0.1 mL …

SCS110 competent cells for generating unmethylated DNA improve quality and yield of miniprep DNA. Transformation efficiency of = 5 x 106 transformants/µg DNA Propagated DNA digestible by methylation-sensitive enzymes such as Xba I, Cla I, and EcoR II;Endonuclease-deficient cells…

Polymerase chain reaction (PCR) enzymes and reagents provide a wide variety of routine, high-fidelity, and Hotstart transcription enzymes for all your PCR needs. These products support features such as reverse transcriptase cloning, cDNA from an RNA template, and high-fidelity cloning. …

High quality human reference RNA from multiple cell lines; pooled and treated to ensure consistency. Enables greater consistency than RNA references derived from tissue sources. Provides the maximum representation of low, medium, and high abundant gene transcripts. Manufactured at…

AAV-293 cells are an HEK293 cell line optimized for packaging of AAV virions in helper-free systems. AAV-293 cells are derived from the commonly used HEK293 cell line but produce higher viral titers. HEK293 cells are human embryonic kidney cells transformed by sheared adenovirus type 5…

Achieve the highest transformation efficiencies available when cloning large plasmids and ligated DNA. Separate DNA fragments and libraries up to 5000 bp in 1-2 minutes/sample Achieve objective quantitative and purity measurements from as little as 2 µL of DNA, sensitive down to 5…

Easily induce and express phosphorylated proteins, which can be used to screen expression libraries, to affinity purify or blot proteins that interact with the tyrosine-phosphorylated target. Competent cell lines for inducible phosphoprotein expression TKB1 Competent Cells are derived…

qPCR Brilliant II SYBR MM with ROX Fast operation Available in a master mix format, including hotstart enzyme and reference dye;Low-abundance RNA Amplification and detection from total RNA, poly(A+), and synthetic RNA samples The Brilliant II qPCR SYBR master mix with ROX includes the…

Ultracompetent cells for high efficiency cloning of methylated DNA Ultracompetent cells for the highest efficiency cloning of a variety of plasmids Deficient in all known E. coli K12 restriction systems to eliminate cleavage of eukaryotic DNA with methylation patterns that are different…

Deploy IPTG in blue/white selection assays to detect ß-galactosidase activity Used in combination with X-gal to detect ß-galactosidase activity in blue/white selection assays. Can induce protein expression when used with a number of derivatives including DE3-derivatives and…

The AD-293 cell line simplifies the process of producing recombinant adenovirus particles. For use with Agilent InterPlay Adenoviral TAP System and AdEasy vectors Improved adherence to tissue culture dishes over standard HEK293 cells Simplifies the process of producing recombinant…

Cloned Pfu DNA Polymerase for High Fidelity and Difficult, GC-Rich PCR. Highly thermostable Pfu DNA polymerase possessing 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors (1 error per 770,000 bp). Reformulated…

Recombination proficient bacterial strain for production of adenovirus plasmid DNA 300% more recombinants with specialty competent cells Recombination proficient bacterial cell strain Infects dividing and non-dividing cells BJ5183 electroporation competent cells are a…

A high quality mouse reference RNA product, treated to ensure undetectable DNA levels. Offers greater consistency than RNA references derived from tissue sources alone Provides the maximum representation of low, medium, and high abundant gene transcripts. Manufactured at industrial scale…

Agilent PfuTurbo Cx HotStart DNA Polymerase combines high fidelity with improved reliability to amplify problematic systems with greater success. Amplifies problematic systems with greater success Combines high fidelity with improved reliability Tolerates varying PCR conditions with…

Provides the highest efficiency of conversion of RNA to cDNA, fully optimized for qPCR applications. Optimized for real-time quantitative PCR (qPCR) application;Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA;Creates up to 12 kb cDNA in 25 minutes or…

Highest efficiency, simplified packaging tool for libraries constructed from highly methylated DNA. Highest efficiency available (2 x 109 pfu/µg) Absence of restriction activity prevents degradation of methylated DNA Increases library representation Multiple product…

InterPlay Mammalian TAP System provides proven purification of interacting proteins Recover intact interacting protein partners without protease digestion, as SBP and CBP elute easily from their affinity resins Exceptionally pure protein for downstream analysis;Includes all vectors,…